FastQCFastQC Report
Mon 25 Sept 2023
SRR22197708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22197708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39550432
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATCTCGTAT6144511.553588592913473TruSeq Adapter, Index 27 (97% over 37bp)
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1016970.25713246318017463No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT912660.23075854139848587No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT561820.14205154573280007No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATCTCGTATG403400.10199635746077312TruSeq Adapter, Index 27 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)