FastQCFastQC Report
Mon 25 Sept 2023
SRR22197709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22197709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39808264
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATCTCGTAT6354741.5963368812063745TruSeq Adapter, Index 27 (97% over 37bp)
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1001010.25145783800067245No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT907760.2280330536393147No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT566600.14233225543319347No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATCTCGTATG414970.1042421744389557TruSeq Adapter, Index 27 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)