FastQCFastQC Report
Mon 25 Sept 2023
SRR22767485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22767485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8785072
Total Bases351.3 Mbp
Sequences flagged as poor quality0
Sequence length20-75
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG4648355.291191694274104No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1882382.1427029852458808No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1397101.590311382763852No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1273361.4494588092163616No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1067171.2147538460697875No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG962861.0960183365600191No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGC742250.8448991653113371No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC697010.7934027177011185No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC636590.7246269580943674No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC605790.6895674844782149No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC586000.667040634385239No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG474030.5395857882553495No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC445060.5066093937534034No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG426870.4859038150171109No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA418560.4764445869083372Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAGGTCCCTGTTCGGGCGCCA400230.4555796469283348No Hit
TCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGCGGG369610.42072506633980916No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGC346320.3942141851540887No Hit
TCGGGCCCCACGTTGGGCGCCA281040.3199063138014122No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC266110.30291157545436165No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC223650.25457958682638004No Hit
TCAGGTCCCTGTTCGGGCGCC214210.24383408582194888No Hit
ACGCCGGCCTCGCGGGCCGCCGGT204480.23275847938411887No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC188970.21510353017027065No Hit
GCGCAGCGGAAGCGTGCTGGGCCC179840.2047109004911969No Hit
TCGTGCCCCACGTTGGGCGCCA173210.19716400730694067No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC165990.18894552030990752No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCCGC160480.18267351707532958No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC160120.18226373102007587No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC158600.1805335232312268No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGCGGGA156570.17822278519743492No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA154700.17609417429931137No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCC150430.17123365636616295No Hit
AACCCGGCGGCGCGCGTCCGGCCGTGCCGGTGGTC144670.16467707948210328No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGTGTCCC135300.15401125909952704No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGCGGG131860.15009552568265805No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG131420.14959467605957014No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCGC118740.1351611005578554No Hit
GTGGCGGCGGCGGCGGCGGCGGCGGCGGGA118070.1343984431772443No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCC116090.1321446198733488No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC113420.12910537329688362No Hit
TCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGG105330.11989656999965397No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCC99520.11328307838569791No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGA99050.11274807992467221No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCG92380.10515565495649894No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG91500.10415395571032315No Hit
ATGTACACGGAGTCGACCCAACGCGA90570.10309534173425101No Hit
CAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG89880.10230991846168136No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)