FastQCFastQC Report
Mon 25 Sept 2023
SRR22767486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22767486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10632411
Total Bases438.7 Mbp
Sequences flagged as poor quality0
Sequence length20-75
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG7674737.218240528888509No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC3954043.7188554881860756No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC3121332.935674702567461No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG2631962.475412208952419No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC2515952.3663024313112047No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC2284992.149079827707939No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2208222.0768760726047932No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1974951.8574808667573142No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1132041.0647067725278867No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1028410.9672406380829335No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC902760.8490642432840491No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC794520.7472623095551894No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC697070.6556085915038461No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC627690.5903552825412787No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC588140.5531576986630784No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC544080.5117183675461756Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGCTTCTGGCGCCAAGCGCCCGGCCGC395730.37219215848597276No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG385780.36283397998817013No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCC382520.3597678833145182No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCC371140.3490647605702977No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCC333610.31376702800521916No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGG283450.2665905221308695No Hit
TCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGCGGG280300.263627882706942No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC272270.2560755034770571No Hit
TCGGGCCCCACGTTGGGCGCCA264860.24910624692743724No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG262080.24649160007076476No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC254420.2392872134081348No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGCGGGA209820.1973400012471301No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG189570.17829446209331073No Hit
TTGGCGGCGGCGGCGGCGGCGGCGGCGGG182600.1717390345425887No Hit
ACGCCGGCCTCGCGGGCCGCCGGT180530.16979215720686494No Hit
GTGGCGGCGGCGGCGGCGGCGGCGGCGGGA171570.16136509395658236No Hit
TCAGGTCCCTGTTCGGGCGCCA170460.16032111625481746No Hit
TCGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGT168280.15827078166936925No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGC164340.1545651310883298No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA164200.15443345822504415No Hit
GCGCAGCGGAAGCGTGCTGGGCCC154190.14501884850011912No Hit
AACCCGGCGGCGCGCGTCCGGCCGTGCCGGTGGTC153050.143946655184793No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG151860.14282743584686483No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG149220.14034446185347801No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG148690.13984598601389656No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCGC129100.12142119035842389No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCC123260.11592855091850758No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG116730.10978695236668334No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCC109540.1030246103165124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)