FastQCFastQC Report
Mon 25 Sept 2023
SRR22767487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22767487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7310600
Total Bases271.9 Mbp
Sequences flagged as poor quality0
Sequence length20-75
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1783612.439758706535715No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC830581.1361310973107541No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC624980.8548956310015593No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA586430.8021639810685854No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG583680.7984023199190217No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG536890.7343993653051734No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGC347550.475405575465762No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGC335690.4591825568352803No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATAC320250.43806253932645745No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG299750.4100210653024376No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG298900.408858370038027No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT264220.36142040325007524Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG229520.3139550789264903No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG217610.29766366645692555No Hit
AGAATCCCGCCCAGGCGGAACGATAC194350.2658468525155254No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAAC181130.2477635214619867No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC180100.24635460837687742No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC178350.24396082400897326No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC173430.2372308702432085No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGTGTCCC164450.2249473367439061No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC162230.22191065028862197No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC156330.21384017727683088No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGC144110.1971247230049517No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGAT143840.19675539627390365No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC143230.19592099143709135No Hit
AGAATCCCGCCCAGGCGGAACGAT141960.1941837879243838No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGA131740.1802040872158236No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGAT128230.17540283971219872No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGAT126990.17370667250294095No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC124830.1707520586545564No Hit
ATGTACACGGAGTCGACCCAACGCGA122600.1677016934314557No Hit
TCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGCGGG116430.15926189368861654No Hit
ACCTCTAGCATCACCAGTATTAGAGGC108350.14820944929280772No Hit
GCGCAGCGGAAGCGTGCTGGGCCC92860.1270210379449019No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG88660.121275955461932No Hit
ACGCCGGCCTCGCGGGCCGCCGGT80240.10975843296035893No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC79330.10851366508904878No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC78340.10715946707520586No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)