FastQCFastQC Report
Mon 25 Sept 2023
SRR22767488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22767488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11609874
Total Bases414.2 Mbp
Sequences flagged as poor quality0
Sequence length20-75
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG3207242.7625106008902423No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1985011.7097601576037778No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1710421.473245962876083No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1041270.8968831186281608No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGA834300.7186124500575976No Hit
ATGTACACGGAGTCGACCCAACGCGA694490.5981890931805117No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC685580.5905145912866927No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC671460.5783525299240974No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG570100.4910475341937389No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA567770.48904062180175256No Hit
TCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGCGGG432100.3721831950975523No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC407360.35087374763929396No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG407110.35065841369165596No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGC389280.3353007965461124No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC385050.3316573461520771No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC303910.2617685601066816No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC300300.2586591379027886No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG232170.19997633049247562No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG196990.16967453738085359No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG188410.16228427629791675Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC183620.15815847786117232No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCCGC180120.15514380259424004No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC172660.14871823759672156No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG164130.14137104330331232No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC154160.13278352547150812No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGC149610.1288644476244962No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC148300.12773609773887296No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAAC138550.1193380737809902No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC135320.11655595917750702No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGTGTCCC130490.11239570730914047No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)