FastQCFastQC Report
Mon 25 Sept 2023
SRR22767489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22767489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13875033
Total Bases478.9 Mbp
Sequences flagged as poor quality0
Sequence length20-75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2662491.918907147824441No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1397271.0070390463215475No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1314240.947197747205358No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA822170.592553545638414No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG574360.4139521686182656No Hit
ATGTACACGGAGTCGACCCAACGCGA547330.39447113387045635No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC466780.3364172178905809No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGA448140.3229830156079629No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC417630.3009938787172614No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG330380.23811114539331185No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC329930.23778682184035166No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC301260.21712380792175412No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG288500.20792743339781605No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC264210.19042116872803114No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA253730.18286803353909142Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAATCCCGCCCAGGCGGAACGATAC250230.18034551701606763No Hit
TCAGGTCCCTGTTCGGGCGCCA231830.16708428729502842No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC214520.15460864129115945No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG198120.14278884958327667No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG182660.13164653374157742No Hit
TCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGCGGG178680.1287780720953961No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATAC174330.12564294441678087No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGC174210.12555645813599148No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG170010.12252943830836295No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC167780.12092223492369351No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAAC166700.12014385839658903No Hit
ACCTCTAGCATCACCAGTATTAGAGGC165970.11961773352178694No Hit
AGAATCCCGCCCAGGCGGAACGAT160330.11555287832468579No Hit
TCAGGTCCCTGTTCGGGCGCC158590.11429882725323967No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGTGTCCC152570.1099600988336388No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC142320.10257272901621206No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGC141260.10180876686923916No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)