FastQCFastQC Report
Mon 25 Sept 2023
SRR22767490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22767490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15087981
Total Bases546.2 Mbp
Sequences flagged as poor quality0
Sequence length20-75
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG5527623.663591570005291No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC3204872.1241211796329806No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC2875771.906000544406836No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1712061.13471775978509No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1607341.0653115218000342No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGA1454860.9642509491495251No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1347060.8928033512237323No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1242570.8235495524550304Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCG1215620.805687652973582No Hit
ATGTACACGGAGTCGACCCAACGCGA1213460.80425604989826No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1133390.7511873192311151No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1132770.7507763961261616No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG814810.54003912120515No Hit
GTCTAGCGGTTAGGATTCCTGGT762210.505176935204253No Hit
GTCTAGCGGTTAGGATTCCTGG559470.3708050798844458No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC530350.35150494953566025No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC456590.3026183556302198No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC445350.2951687174049331No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCC439060.2909998362272593No Hit
GTCTAGGGGTATGATTCTCGCTT438200.29042984611393663No Hit
TCAGGTCCCTGTTCGGGCGCCA409440.27136831627770475No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCC397560.2634944993634337No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCC366890.2431670612522643No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT357340.23683751987757673No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC356160.23605544041976192No Hit
GCGCAGCGGAAGCGTGCTGGG331930.21999630036649703No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG293960.19483057408410046No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG289610.1919474845574103No Hit
TCGTTTCCCGGTCAGGGAACCA287910.19082075991479575No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG282570.18728151897858303No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC281360.1864795561447221No Hit
GCGCAGCGGAAGCGTGCTGG263300.17450976376494642No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG252200.167152914627875No Hit
GCGCAGCGGAAGCGTGCTGGGC250560.16606595673735275No Hit
TCGGGCCCCACGTTGGGCGCCA236270.1565948419473752No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC229890.15236631064156297No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG200190.13268176835588538No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA183950.12191823412290882No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGG181970.12060593130386364No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC171600.11373291098391494No Hit
TCGTGCCCCACGTTGGGCGCCA169230.11216212427626997No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC164990.109351940461749No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGTGTCCC161140.10680024053582782No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGA157370.10430156294602969No Hit
TCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGCGGG154810.10260484819009251No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG153380.10165707393189322No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC151260.10025198202463272No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)