FastQCFastQC Report
Mon 25 Sept 2023
SRR22969806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22969806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21205830
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC4322332.0382743802058205No Hit
GGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC4311932.0333700685141776No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG2648111.2487650801689911No Hit
CCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC2603441.2277001183165195No Hit
CCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC2442611.1518577674158474No Hit
GGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1970360.9291595754563722No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1933070.9115747886312395No Hit
GGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1838330.8668983953940967No Hit
GCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1725540.8137101919613615No Hit
GCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1685270.7947201312092004No Hit
CGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1574770.7426118194854905No Hit
CGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1541950.7271349435509009No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1540510.7264558850089811No Hit
GCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1507780.7110214502332614No Hit
CCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1456590.6868818622048748No Hit
CGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1415470.6674909682856082No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1415110.6673212036501284No Hit
CGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1266360.5971753994066726No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1224980.577661897695115No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1166050.5498723700039093No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1120950.5286046337257254No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA1119850.5280859084506478No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1114340.5254875663909406No Hit
GGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1093730.5157685410097128No Hit
GCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1072900.5059457705734696No Hit
CCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1003790.4733556762456362No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG962320.4537997333752086No Hit
GGGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG924600.43601217212436394No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG904140.42636388200791947No Hit
GGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA896590.4228035403471593No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG837080.3947405029654581No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG818010.3857476929693391No Hit
CCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG808930.381465851607789No Hit
CGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC794710.37476014850633055No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG775810.3658475051436327No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG694280.32740053089174065No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG615820.29040127172574715No Hit
GGGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT576260.27174602456022706No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG575760.27151024034428267No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG573910.27063783874528846No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA573150.27027944673705295No Hit
GGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT557420.2628616753034425No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT554010.26125362695070176No Hit
GCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG553700.26110744073681624No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG533870.2517562387324618No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG498140.23490709866107576No Hit
CCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA489080.23063468866816342No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA464390.2189916640848295No Hit
GGGTTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAG458360.2161481064405402No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG440290.20762686487630996No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG424760.2003034071290772No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT423440.19968093679898405No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG414780.19559715417882725No Hit
GGGGGCCTCGAGCTTTACAGCGCCCCCCCGCCCCGACTTCGCCGCTTGCA401910.18952806846041867No Hit
GGCTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG381940.18011084687559978No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG377740.1781302594616669No Hit
GGGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAA375560.17710224028014937No Hit
GGGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA371120.1750084764425632No Hit
CCGTTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAG370560.1747443981207055No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA363470.17140097793861406No Hit
CGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA363130.17124064467177186No Hit
GGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG357140.1684159497647581No Hit
CCGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG354480.16716157773593393No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG345430.1628938834273405No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA334250.15762174835882398No Hit
CCCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT328370.15484892597931796No Hit
GGCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA322510.1520855349684497No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC321740.15172242727589536No Hit
GGGGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAA321040.1513923293735732No Hit
CCCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA319890.15085002567690112No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT317410.14968053596581696No Hit
GGGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG312450.1473415565436486No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC300360.1416402942021133No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG298630.1408244808149457No Hit
CCGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA295230.13922114814652387No Hit
GGGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG290750.13710852157166215No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA289990.13675012956342666No Hit
CGCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA284050.13394901307800733No Hit
CGCTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG276900.13057729879000257No Hit
GCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA273660.12904941707068293No Hit
CGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA266470.12565884004540262No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC263750.12437617391066512No Hit
GGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA262890.12397062505924078No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG260710.12294260587772325No Hit
GCGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG260060.12263608639699554No Hit
GGGTGCTGCGATCCATTGAAAGTCAGCCCTCGATCCAAGTTTTGTAAAAA254530.1200283129686506No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG248500.11718475532436128No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA246600.1162887753037726No Hit
GGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC246280.11613787340556819No Hit
GGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGAT245290.1156710206579983No Hit
CGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG241350.11381304103635652No Hit
CGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGG240890.11359611955768767No Hit
GCGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA233830.1102668464285529No Hit
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA232940.10984715052417189Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG230800.10883799407992992No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA229360.10815893553801008No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAAAAAAAAAAAA227800.10742328878426358No Hit
CCGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA226150.1066452008716471No Hit
GGGCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG226130.10663576950300932No Hit
CCGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC225370.10627737749477385No Hit
CGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG221770.10457973113997424No Hit
GGGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAG220110.10379692754303887No Hit
CGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG219810.10365545701347223No Hit
CCGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT217050.10235392814145922No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG215140.10145323243655165No Hit
GGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG213350.10060912494347073No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)