FastQCFastQC Report
Mon 25 Sept 2023
SRR22969808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22969808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38336224
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG7446001.9422883171801166No Hit
GGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC4771281.2445878863812982No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA4710471.2287256042744326No Hit
GGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC3886791.0138687628703338No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG2739040.7144782960366676No Hit
CCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC2651700.6916956662189787No Hit
CCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC2323050.6059673482709199No Hit
GGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC2106440.5494646525437664No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG2100770.5479856336398702No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG1930360.5035342030555748No Hit
GCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1810010.4721409182083244No Hit
CGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1745790.45538913795996183No Hit
GGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1742730.4545909372816686No Hit
GGGGGCCTCGAGCTTTACAGCGCCCCCCCGCCCCGACTTCGCCGCTTGCA1736260.4529032384618788No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1686770.439993777164908No Hit
CGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1681800.43869735318741876No Hit
CCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1643400.4286807172245237No Hit
GGGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG1523770.3974752443015776No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1518540.39611099935142285No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1458660.3804913076467834No Hit
CGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1419300.37022425578481594No Hit
GCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1413540.3687217603903817No Hit
GGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1361300.35509496188252654No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1357060.35398895832829025No Hit
GCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1305610.3405682312373801No Hit
GGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT1301490.3394935296705278No Hit
CCGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA1293020.3372841310610038No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1280950.3341356728299584No Hit
GCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1225110.3195698147005819No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1161050.30285977043539813No Hit
CGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1153510.30089296222810047No Hit
CGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA1121590.29256663358394397No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1120660.2923240431817176No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA1091870.284814174708495No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1081430.2820909018060829No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1042730.27199601087472774No Hit
GGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG1036850.27046221349290944No Hit
GGGGGCCTCGAGCTTTACAGCGCCCCCCCGCCCCGACTTCGCCGCTTGAA1030090.26869886820360817No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG1017230.2653443385556178No Hit
CCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG983690.2565954330817766No Hit
GCGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA982080.2561754647510407No Hit
GGGCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG965410.25182709700360684No Hit
GGGGGCCTCGAGCTTTACAGCGCCCCCTCGCCCCGACTTCGCCGCTTGCA944810.24645358916934543No Hit
GGGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG939060.24495370227385982No Hit
CGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC917550.2393428210352694No Hit
GGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA895020.23346587290391457No Hit
GGGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG890500.2322868313791155No Hit
CCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC861720.22477957140484153No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG851740.22217628945406828No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA849650.22163111317379616No Hit
GGGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT815080.21261353230824193No Hit
GCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG802780.2094050786013771No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG780640.20362986192902044No Hit
CGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG763710.19921367320892117No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG759810.19819635861893964No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG735010.19172728122623656No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG732790.1911481944596317No Hit
GGCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG731820.19089517006161066No Hit
CGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA724930.1890979142859766No Hit
GGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG724780.18905878680174656No Hit
CCCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT717850.18725109703031784No Hit
GGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGAT704750.1838339634075594No Hit
CCCCCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAACCCGGAG687830.17942038318640877No Hit
GCGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG673970.17580500364355134No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA666120.1737573319688449No Hit
CGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG647760.16896812789908572No Hit
GGCTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG646880.16873857999160274No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG635050.1656527257353254No Hit
CCGCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG632770.16505798797502852No Hit
CCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG628120.1638450359638967No Hit
GGCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG616430.16079570069290078No Hit
CCGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG610600.15927494580582585No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA606260.1581428572621028No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT606150.15811416377366744No Hit
GGGGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAA604440.15766811045344475No Hit
CCGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG596520.15560217928609765No Hit
CCGGGGGCGGGGGGTGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT595780.15540915036389605No Hit
GGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC594410.15505178600792816No Hit
CGCCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG584790.15254241001930707No Hit
GGGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA584030.1523441640992081No Hit
GGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA572210.14926091834187946No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA551930.14397088247397552No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG548320.14302921435350543No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG545450.14228057515523698No Hit
CCGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG544590.14205624424565133No Hit
GGGGGCCTCGAGCTTTACAGCGCCCCCTCGCCCCGACTTCGCCGCTTGAA531820.13872519108819897No Hit
CCGGGCCTCGAGCTTTACAGCGCCCCCCCGCCCCGACTTCGCCGCTTGCA531600.13866780411132823No Hit
GGGGGCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG527470.13759049404552728No Hit
CGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG524330.1367714253756447No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG519040.13539152943179797No Hit
CCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA514910.13431421936599702No Hit
CCCCCCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAACCCGGA507310.1323317601650074No Hit
CGCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG484340.12634003807991107No Hit
GCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG473590.12353590171009017No Hit
GCGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG469460.12245859164428924No Hit
GCGCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG464290.12110999768782653No Hit
CGCTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG462780.12071611434657727No Hit
GCCCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCGGA461430.12036396698850674No Hit
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA460040.12000138563464155Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA458570.11961793628918696No Hit
CGCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG451290.11771894905455477No Hit
GGGGGGCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAA445080.11609907120743033No Hit
GGGGGAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGGGGGCG443990.11581474482202525No Hit
GGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT442520.11543129547657069No Hit
GCCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG441940.11528000253754778No Hit
CCCCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCGGA435890.11370186067360206No Hit
GGGGGGGGCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATC433880.11317755238491928No Hit
CCGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT431650.11259585711936575No Hit
GGCCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG427150.11142203259246398No Hit
CCCCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG426740.11131508413556848No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA425620.11102293225331739No Hit
GGGGGCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA424600.11075686536055299No Hit
GGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGA423140.11037602451404707No Hit
CGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT421950.11006561313915528No Hit
GCGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG419320.10937957791565492No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAAA411680.10738668471887058No Hit
GCGGGGGCGGGGGGTGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT406230.10596505279184512No Hit
CGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT401150.10463993532592046No Hit
GGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC398110.10384695164552461No Hit
GGGGGGGGCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGG393800.10272268859864758No Hit
GCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA393080.1025348766743433No Hit
GGGGGGGCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGA390950.10197926639827647No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG387620.10111063624836916No Hit
GCGGGCCTCGAGCTTTACAGCGCCCCCCCGCCCCGACTTCGCCGCTTGCA385680.10060458745232707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)