FastQCFastQC Report
Mon 25 Sept 2023
SRR22969809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22969809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27108464
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG6479112.390069020509609No Hit
GGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC4045481.492330956117617No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA3808891.4050556313334464No Hit
GGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC3215001.1859764537009547No Hit
CCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC2252740.8310098277792501No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG2168010.7997539071191934No Hit
CCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1981840.7310779393476516No Hit
GGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1800790.6642906805785824No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1625780.5997315082108673No Hit
GCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1569950.5791364645374227No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG1513650.5583680432797669No Hit
CGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1422620.5247881252143243No Hit
GGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1414100.5216451953898974No Hit
CCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1374040.5068675229994588No Hit
CGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1364760.5034442379324775No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1341170.49474215875897654No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1234240.4552969139085121No Hit
GGGGGCCTCGAGCTTTACAGCGCCCCCCCGCCCCGACTTCGCCGCTTGCA1223360.45128340727825816No Hit
GGGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG1197920.44189888442222325No Hit
GCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1197750.4418361733811256No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1186240.437590267010333No Hit
CGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1168560.43106831873617035No Hit
GGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1154840.42600716883110756No Hit
GCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1092070.4028520391269679No Hit
GCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1053050.38845801075265646No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1050000.387332900897668No Hit
CCGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA1022910.3773397120545081No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1001350.36938647648940937No Hit
CGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC959350.35389316045350266No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA891860.3289968771377087No Hit
GGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT886510.32702332378551585No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG877300.32362586091192774No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG872130.321718707485603No Hit
CGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA867580.32004026491504645No Hit
CCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG825310.3044473489903375No Hit
GGGCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG824490.3041448604391603No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG822670.30347348341093766No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG801990.29584486970563884No Hit
GCGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA795620.2934950501068596No Hit
GGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG791540.2919899851205144No Hit
CGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC747630.27579209209345096No Hit
CCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC706280.26053855356762373No Hit
GGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA706040.26045002033313286No Hit
GGGGGCCTCGAGCTTTACAGCGCCCCCCCGCCCCGACTTCGCCGCTTGAA693590.2558573587939177No Hit
GCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG680250.25093638651013206No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA673990.24862714464382787No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG673180.24832834497742107No Hit
GGGGGCCTCGAGCTTTACAGCGCCCCCTCGCCCCGACTTCGCCGCTTGCA636940.23495982656929587No Hit
GGGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG633970.23386422779247104No Hit
GGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG629120.23207511867880085No Hit
CGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG594310.21923411079285052No Hit
CGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA589190.2173454017903781No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG567820.20946225503591792No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG567340.20928518856693615No Hit
GGGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT562040.2073300796385955No Hit
GGGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG556430.20526061528237083No Hit
GGCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG553580.20420928312279146No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA542560.2001441321057512No Hit
CGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG540900.19953177723385582No Hit
GCGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG540670.1994469328841354No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG538850.19877555585591278No Hit
CCGCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG532140.19630031417493815No Hit
CCCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT520300.19193267460672062No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG497800.18363268387319914No Hit
GGCTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG497430.18349619513669235No Hit
CCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG493550.1820649078457562No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA493160.18192104133970852No Hit
GGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGAT488230.1801024211478747No Hit
CGCCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG485680.17916175553140892No Hit
CCGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG465510.17172127494940326No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG461400.17020514330874667No Hit
CCCCCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAACCCGGAG458230.16903576683651275No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA445990.1645205718774771No Hit
CGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG436500.16101982023031627No Hit
GGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA436250.1609275981110549No Hit
GGGGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAA432850.15967337728910055No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG428270.15798386806423265No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT417660.15406996132278097No Hit
CCGGGCCTCGAGCTTTACAGCGCCCCCCCGCCCCGACTTCGCCGCTTGCA412780.15226978555479942No Hit
GGCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG412620.15221076339847214No Hit
CCGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG411930.15195623034931083No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG408650.15074627614460193No Hit
GGGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA407980.15049912086498152No Hit
GGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC404550.1492338333887158No Hit
GCGCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG398890.14714592460863882No Hit
CCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA383860.14160153079864649No Hit
CGCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG373090.13762860190086756No Hit
GCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG370760.13676909174935178No Hit
GGCCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG363090.13393971713041358No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA362390.13368149519648181No Hit
GGGGGCCTCGAGCTTTACAGCGCCCCCTCGCCCCGACTTCGCCGCTTGAA362160.13359665084676137No Hit
CCCCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG360380.13294002935762056No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG356470.13149767541237306No Hit
CGCTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG353550.13042052105940047No Hit
CCCCCCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAACCCGGA350200.1291847446612984No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA349950.12909252254203707No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG343870.12684968060160104No Hit
CCGGGGGCGGGGGGTGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT339000.12505319371838997No Hit
GCGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG335110.12361821754268335No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAAA333660.12308332925096753No Hit
GGGGGCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG330440.12189550835488133No Hit
CCGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG329240.12145284218242686No Hit
GCGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG322480.11895915607759996No Hit
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA322450.1189480894232886Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT322190.1188521784192568No Hit
CGCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG320470.11821769023873871No Hit
GGGGGGGGCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATC319970.11803324600021602No Hit
GCCCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCGGA317100.11697453607109572No Hit
CCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG312510.11528133796145734No Hit
GGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGA304150.11219743029335781No Hit
CGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT303000.11177320854475561No Hit
CCCCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCGGA299920.1106370320354558No Hit
GGGGGCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA299500.11048209887509672No Hit
GCCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG296270.1092905890942401No Hit
CGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT295850.10913565593388103No Hit
GCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA294430.10861183429647656No Hit
GGGGGAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGGGGGCG292340.10784085737945168No Hit
GGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGAA291200.1074203245156199No Hit
GCCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG288800.10653499217071097No Hit
GCGGGCCTCGAGCTTTACAGCGCCCCCCCGCCCCGACTTCGCCGCTTGCA288460.10640957008851552No Hit
CCGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT282000.10402655052680226No Hit
GGCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA278660.10279446301347062No Hit
GGGGGGCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAA272590.10055530995780507No Hit
GGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC272380.10047784337762554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)