FastQCFastQC Report
Mon 25 Sept 2023
SRR22969810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22969810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35540295
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC8927242.5118643500286084No Hit
GGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC6000251.688294933961578No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG5380951.5140420190659645No Hit
CCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC4997571.406170095099098No Hit
GGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA4367691.2289402775075446No Hit
GGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC4247591.1951476486056178No Hit
GCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC3448440.9702902015866779No Hit
CCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC3325030.9355662354519005No Hit
CGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC3298140.9280001755753575No Hit
CCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC3191690.8980482576185707No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG2925810.8232373985640806No Hit
CGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG2832010.7968448207872219No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2666590.7503004688059004No Hit
GGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC2429950.6837168909262008No Hit
GCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC2364050.665174557498749No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG2306080.648863494239426No Hit
CCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA2214590.6231208829302064No Hit
GGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG2175690.6121755601634707No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2108420.5932477487876788No Hit
GGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG2054210.577994639605552No Hit
CGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC2002740.5635124863201052No Hit
GCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1929460.5428936366453908No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1764820.49656875386093446No Hit
GGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT1764030.4963464709564172No Hit
CGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1761170.49554175056791167No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1696790.47742710070358163No Hit
GCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1689450.4753618392869277No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA1648870.4639438136346364No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1630660.4588200520001311No Hit
CGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1610590.45317294074233205No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1575970.44343188485070256No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1509100.42461662178099535No Hit
CCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1493410.4202019144748236No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1348840.3795241429481663No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG1308210.3680920487576144No Hit
CGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA1305000.3671888486012848No Hit
GCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA1285240.3616289622807014No Hit
GGGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG1250240.35178098549829145No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1243950.3500111633851098No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT1223840.34435279729670226No Hit
CCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG1209800.3404023517531298No Hit
GGGGGAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGGGGGCG1177040.3311846454847941No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1165790.3280192243761623No Hit
GCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1129260.3177407503229785No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1127700.31730181192924817No Hit
CCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1126190.3168769420737785No Hit
CCCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT1087010.30585283549278364No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1056680.29731886018391235No Hit
GGGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT1043070.29348940406938095No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG996830.2804788198859914No Hit
GCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG902790.2540187131254819No Hit
GGGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG847420.2384392138557094No Hit
GGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGA810020.22791594723679137No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG808010.22735039199871582No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG802690.22585349952778952No Hit
GGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGAT784250.22066502261728552No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG767990.21608993397494308No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA763580.21484908890035945No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG703310.1978908728810495No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG697930.1963770981642105No Hit
GGGGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAA697570.19627580468873426No Hit
CGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA683480.19231129060690128No Hit
GGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT675920.1901841276219007No Hit
GGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC675520.19007157931581603No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC648630.18250551943927307No Hit
CGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT626460.1762675295745294No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA618760.17410097468239924No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG618060.17390401514675102No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG611010.17192035125200847No Hit
CGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT597870.16822313939712655No Hit
GGCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG577290.16243252904906952No Hit
CCGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT557780.156942985419789No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG555450.1562873915368457No Hit
CCGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG546170.15367627083568103No Hit
GGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA543690.15297847133795597No Hit
GGGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA532520.14983555989054115No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC513500.1444838879362144No Hit
GGCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG510140.14353848216510304No Hit
CGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG507650.14283786895972586No Hit
CCGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC503520.1416758076994015No Hit
GGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAAA496530.13970902605057162No Hit
GGCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA496040.13957115437561787No Hit
GGCTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG494860.13923913687266803No Hit
CCCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA482690.1358148546600415No Hit
CGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG482190.13567416927743567No Hit
GGGCGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG465490.13097527749840004No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG463550.13042941821388931No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA463000.1302746642930229No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG458310.12895503540417996No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA453190.127514417086296No Hit
GGGGGCCTCGAGCTTTACAGCGCCCCCCCGCCCCGACTTCGCCGCTTGCA452430.1273005753047351No Hit
GGGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG451950.12716551733743348No Hit
GCGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG449670.12652399199275077No Hit
GGGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAG448180.12610474955258533No Hit
CGCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG447770.1259893875388485No Hit
CCGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG447110.12580368283380877No Hit
CGCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA434660.12230061680692296No Hit
CCGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA429670.12089657668851651No Hit
GCGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG429080.1207305679370416No Hit
GGGTTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAG420490.118313593063873No Hit
GGGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC403420.11351059410170906No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG402070.11313074356867325No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA399090.11229225868834235No Hit
GCCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG392340.11039300602316328No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGAAAAAAAAAAAAAAAGATCGG389270.10952919777396333No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC388620.10934630677657572No Hit
CGCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG383770.10798165856529891No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC383550.10791975699695232No Hit
CGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAAA366040.10299295489809525No Hit
CGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGG363780.10235705696871678No Hit
CCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC360540.10144541568943083No Hit
GGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG355930.10014829646180484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)