FastQCFastQC Report
Mon 25 Sept 2023
SRR22969811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22969811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27977708
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC3804891.3599720177221093No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG3419391.2221837471461208No Hit
GGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC3279691.1722511365119688No Hit
GGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA3042311.0874050154501576No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2777410.9927224917780971No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG2454130.8771733553012991No Hit
CCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC2144090.7663565578710021No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2083880.7448358528868769No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2013150.719555011439822No Hit
GGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1877990.6712451212944248No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1790230.6398772908774372No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1783360.6374217644990791No Hit
CCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1723610.6160654761283518No Hit
GCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1545780.5525041579531819No Hit
CCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA1512790.540712627353177No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1460020.5218511823770553No Hit
CCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1401720.5010131637659525No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1398750.4999516043272737No Hit
CGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1385260.49512990842566523No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1335140.47721564611368456No Hit
GGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1296240.46331171945893496No Hit
CGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1192610.42627151587971396No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1100990.3935240156198642No Hit
CGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1066820.3813107206637513No Hit
GCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1056240.3775291385555957No Hit
GCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1052170.3760744089544433No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG990130.3538996117909301No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA969070.3463721903166621No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG967270.34572882095988705No Hit
GGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG946320.33824071650186643No Hit
CGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC923580.33011281696127504No Hit
GGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT921010.3291942284907684No Hit
GGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG916220.327482151146906No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG909640.32513027872047273No Hit
GCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC908330.32466204879970867No Hit
CGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA896170.3203157313672728No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG891540.3186608424106792No Hit
GGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGAT885610.31654129780752593No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG870080.310990449968239No Hit
CCGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC868990.3106008540799697No Hit
GCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA863220.3085384978640852No Hit
GGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGA860290.3074912355222236No Hit
GGGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC742150.2652647600725549No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG726970.2598390118304187No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG723160.2584772133585782No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG720180.25741207964569507No Hit
GGGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG714840.2555034172205958No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC706200.25241524430807555No Hit
CGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC681480.24357963847503164No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG672550.24038781161058653No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT647110.23129485803483257No Hit
GGGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG633620.22647316213322408No Hit
GGGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA628190.22453233124028601No Hit
CCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG626940.2240855469647478No Hit
CCCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT621000.22196242808739014No Hit
GGGGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAA595790.21295168281833524No Hit
CCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC592860.21190442047647365No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC573090.20483808037456108No Hit
CCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG535230.1913058782370593No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG532780.19043018105700438No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG528970.18906838258516387No Hit
GGGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT527130.18841071613157162No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGAAAAAAAAAAAAAAAGATCGG521230.1863018943510312No Hit
CGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGG502710.17968233852465684No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG502390.17956796175011905No Hit
GCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG493810.17650123448282468No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA484310.17310567398873417No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG479530.171397170919076No Hit
GGCCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC458310.16381256105753908No Hit
GCGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC457300.1634515593629042No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGAAAAAAAAAAAAAAAGATCGG445950.1593947581410171No Hit
GGGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAG442800.1582688617666608No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG430410.15384033602752592No Hit
GGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGG424250.15163858311767353No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC423440.15134906690712477No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC420490.15029465601685457No Hit
GGGGGAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGGGGGCG419020.1497692377088216No Hit
GGGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG410340.14666676769948417No Hit
GCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG400510.1431532561566516No Hit
CGCCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC400140.14302100801109224No Hit
GGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT389080.13906786074113006No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG387650.13855673952991432No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG380050.1358402911346419No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA378770.13538278403649076No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC377350.13487523709947935No Hit
CGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA375080.1340638768551019No Hit
GGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC369640.13211947168795957No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG365420.13061112797374252No Hit
CCGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG359950.12865599998398727No Hit
GGGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGGA357120.1276444803841687No Hit
CCGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA356970.12759086627110414No Hit
GGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAAA356640.12747291522236204No Hit
CGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT346250.1237592443240883No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG344320.12306940940265729No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG326450.11668218139956282No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT322750.11535969994396968No Hit
CCCCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC321430.11488789574900131No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGAAAAAAAAAAAAAAAGATCGG320730.11463769655469991No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGAAAAAAAAAAAAAAAGATCGG320250.11446613139289323No Hit
CGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT319760.11429099195688225No Hit
CCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGGA318000.11366191969692442No Hit
GGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA309670.1106845492847377No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC309180.1105094098487267No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA301250.10767501040471221No Hit
GCCCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC300420.10737834564575482No Hit
GGCCCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA291370.10414362749085809No Hit
CCCCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG287880.10289620579355535No Hit
GGCCCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA282290.1008981865133484No Hit
GGCTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG281680.10068015578688576No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGAAAAAAAAAAAAAAAGATCGG280840.10037991675372407No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAAAAAAAAAAAAAAAGATCGGAA280100.10011542046260544No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)