FastQCFastQC Report
Mon 25 Sept 2023
SRR22969814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22969814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36341685
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG5159031.4195902033711425No Hit
GGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC4149541.141812769550999No Hit
GGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC3543070.9749327803595238No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2687810.7395942152929893No Hit
CCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC2385740.656474789212443No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG2325930.6400171043252397No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2162750.5951154989098607No Hit
CCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC2003490.5512925446357262No Hit
GGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG1913590.5265551115750411No Hit
GGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1787710.491917201967933No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1759360.48411624282143223No Hit
GGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA1686110.4639603254499619No Hit
GGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT1623590.4467569404115411No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1589010.4372416964155625No Hit
GGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1568100.4314879731085667No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1526080.41992549327308293No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1458640.40136829098595733No Hit
CGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1458070.40121144630470496No Hit
GCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1414080.38910688923752434No Hit
CGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1369770.3769142790159565No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1360920.37447905896493244No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1326230.36493354669713307No Hit
GCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1323750.36425113475063137No Hit
GCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1317090.3624185284749455No Hit
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1308300.3599998183903691Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1279910.35218785259956986No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1264460.34793653623930754No Hit
CGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1256320.3456966841245804No Hit
CGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1221970.33624472833331753No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1179610.3245886920212973No Hit
CCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG1175460.3234467526753369No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1147740.3158191481765361No Hit
GGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1094940.3012903777026299No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1033960.2845107484696981No Hit
CCCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT983640.2706643899422935No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG976390.26866943566320606No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG975810.2685098393208791No Hit
CCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC920070.25317208049103945No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA916840.2522832939639425No Hit
CCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA913090.2512514210609662No Hit
CCGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC912520.25109457637971383No Hit
GCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC896000.24654883228446894No Hit
GGGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG888440.2444685765120687No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG870200.23944954671199203No Hit
GCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG860860.23687949526831242No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG856300.23562473781829327No Hit
CCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG776520.2136719857651069No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG769310.21168803813031786No Hit
GGGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC752690.2071147774243269No Hit
GGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC739610.2035156047387456No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT726290.1998503921873738No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT699550.1924924504738842No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG697170.19183755513812856No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG685560.18864287663051396No Hit
CGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC670140.18439981525347546No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC666350.18335693570620076No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA648160.17835166421149706No Hit
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC644020.17721247652661126Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG620640.1707790929341884No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG614790.16916937120554537No Hit
GCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG607850.16725971841977058No Hit
GGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT587920.1617756578980859No Hit
GGGGGAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGGGGGCG579620.15949177920616503No Hit
GGGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG558520.15368577433875177No Hit
CGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT549970.1513331041199658No Hit
CGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT539220.1483750684647671No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC530630.14601139160168275No Hit
CCGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT514140.14147390248966166No Hit
GGGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG513140.14119873638220132No Hit
CGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA504140.1387222414150582No Hit
GCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA496340.13657594577686752No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG491640.1352826650718039No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA489030.1345644815313324No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG487420.13412146409832126No Hit
CGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGG483370.13300704136310687No Hit
CGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG467880.12874471835854612No Hit
GCGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC466780.12844203564033974No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG458580.1261856735591649No Hit
GGCCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC454430.12504373421320447No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGAAAAAAAAAAAAAAAGATCGG452560.12452917359225364No Hit
CCCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA434510.11956242535259441No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG431160.11864061889260225No Hit
GGCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA426730.11742163303655292No Hit
CGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG419290.11537439719704796No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT414390.11402608327049227No Hit
GGGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT413600.11380870204559859No Hit
GGGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA404710.11136247535027614No Hit
GGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGG396140.10900430180934098No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC389610.10720746712762494No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC388850.10699834088595507No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG384130.10569955685874224No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC381560.10499237996256916No Hit
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA381400.1049483533853755Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGCGCGCCCCATTGAATACCCCAGCTAGAAAAAAAAAAAAAAAGATCGG379910.10453835588525959No Hit
CGCCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC378680.10419990157308336No Hit
CGCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA376140.10350097966013408No Hit
GGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG375670.10337165158962772No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA374090.10293688913984038No Hit
CCGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG371600.10225172553226412No Hit
CCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC371440.10220769895507047No Hit
GGCTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG369090.10156105860253865No Hit
GGCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG367240.10105200130373702No Hit
GGGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGGA366540.10085938502851477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)