FastQCFastQC Report
Mon 25 Sept 2023
SRR22969815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22969815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40946913
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG7553801.844778872585584No Hit
GGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC6100391.489829037905739No Hit
GGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC5343401.304957958613388No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG3791270.9258988583583823No Hit
CCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC3543170.865308210169592No Hit
CCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC3020090.7375623163582564No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG3017350.7368931572448453No Hit
GGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC2525550.6167864229471951No Hit
GGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC2413940.589529178915148No Hit
GGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG2364910.5775551382835624No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2298860.5614244961518833No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG2266710.5535728664087571No Hit
CGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC2100340.5129422088546699No Hit
GCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC2089750.5103559333032016No Hit
GCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC2086570.5095793179817976No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG2072740.5062017739896534No Hit
CGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1924440.4699841475229159No Hit
CCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1897260.4633462844928017No Hit
GCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1891350.4619029522445318No Hit
CGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1834030.44790433896689597No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1799220.4394030876027211No Hit
CGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1761680.4302351193116805No Hit
GGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA1686810.4119504686470504No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1682110.4108026409707613No Hit
GGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1612750.3938636350925893No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1568210.3829861362198415No Hit
GGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT1461290.35687427767753827No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1420730.34696876904981827No Hit
CCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG1397890.3413908149803625No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1396570.34106844635638345No Hit
GCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1389940.3394492766768523No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1336700.32644707550969715No Hit
CCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1324110.3233723626491697No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1202690.2937193336161874No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1198320.2926520980958931No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1130410.27606720926678896No Hit
CCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1100200.26868936371345015No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1094160.2672142830400914No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA1066550.2604714059885296No Hit
GCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG1030210.25159650008292445No Hit
GGGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG1011060.24691971284868286No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1010780.2468513316254146No Hit
GGGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG995670.24316118775547257No Hit
CGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC993910.24273136292350048No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG920410.22478129181557593No Hit
CCGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC919300.22451020910904812No Hit
CCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA913520.2230986252858671No Hit
CCCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT912870.22293988316042287No Hit
GCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG907600.22165285085105194No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG866730.2116716344404278No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG827410.20206895694432447No Hit
GGGGGAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGGGGGCG766760.1872570955471051No Hit
GGGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG753300.18396991245713687No Hit
GGGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC733240.17907088624727338No Hit
GGGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA731870.17873630669056786No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC729430.17814041317351567No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT714590.1745162083402966No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA694210.16953903216098365No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG690240.16856948410250122No Hit
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA674840.16480851682274558Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC636230.15537923457135827No Hit
CCGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG623060.15216287489120364No Hit
CGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGG621500.15178189379013748No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG607000.1482407232994585No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGAAAAAAAAAAAAAAAGATCGG594180.1451098401483892No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC586820.14331239085105146No Hit
CGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG563110.13752196655215498No Hit
CGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG559420.13662079971694083No Hit
GGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT554850.1355047204657406No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG546940.1335729509084116No Hit
GGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG536840.13110634249766276No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT530510.12956043841449047No Hit
GGCCCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA528410.12904757923997834No Hit
GGGGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAA521590.1273820080160866No Hit
GGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCGG516680.12618289442234634No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG514380.12562119151692827No Hit
CGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT511130.12482748088970712No Hit
CGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG509990.1245490716235434No Hit
CGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT506050.12358685012469682No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA503960.12307643313673No Hit
GCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA501580.12249519273894957No Hit
CGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA501580.12249519273894957No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGAAAAAAAAAAAAAAAGATCGG495220.1209419620961414No Hit
GGCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA493670.12056342318162054No Hit
CCGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG493140.12043398729471987No Hit
CCCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA486970.11892715819627232No Hit
GGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGA486840.11889540977118349No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG479990.11722251198765582No Hit
GGCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG478220.11679024496913845No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG471170.11506850345470489No Hit
GCGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC464280.11338583692499601No Hit
CCGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT452670.11055045834590753No Hit
CCGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA452310.1104625396302769No Hit
CGCCCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA451160.11018168817756788No Hit
GGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGAT443250.10824991862023883No Hit
CGCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA443070.10820595926242353No Hit
GGCCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC439220.10726571744248461No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAAGATC438500.10708988001122333No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG421640.10297235349585449No Hit
GGGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT411710.10054726225637571No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)