FastQCFastQC Report
Mon 25 Sept 2023
SRR22969816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22969816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35697344
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG5769081.6161090304085368No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG4396401.2315762203484943No Hit
GGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC3334030.9339714461669754No Hit
GGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC3139880.8795836463351447No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT2475740.6935361913760306No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG2326330.6516815368672806No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG2108110.590550938467579No Hit
CCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1901470.5326642788886479No Hit
CCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1714630.48032425045403937No Hit
GGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1611510.4514369472417892No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1577530.441918031772896No Hit
GGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA1568530.4393968357982039No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1521300.4261661595887918No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC1464010.4101173465454461No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1366450.3827875821797835No Hit
GCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1310310.36706092195542617No Hit
CCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1300300.3642567917657964No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1292200.36198771538857344No Hit
CGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1264600.3542560477328509No Hit
GGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1262490.35366496734322866No Hit
CGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1189880.33332451848518474No Hit
CGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1154260.3233461850831255No Hit
GGGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAA1152960.32298201233122553No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1139610.3192422383020989No Hit
GCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1105520.3096925082157373No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1046250.2930890320579593No Hit
GCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1041700.29181442742630936No Hit
CGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1038670.29096562478149635No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1037780.29071630651288793No Hit
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1034430.2897778613445303Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT973300.2726533380186492No Hit
GGGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG958520.2685129739624326No Hit
GCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC928390.2600725701049355No Hit
GCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC922190.25833574621125877No Hit
GGGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA907530.25422899810137134No Hit
GGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG900940.25238292238212456No Hit
CGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC891800.24982250780338167No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG884120.24767108723831108No Hit
GGGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG882800.24730131182868956No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG812390.22757715532001482No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG812020.2274735061521664No Hit
CCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA795260.2227784789815175No Hit
CGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT793210.22220420656505988No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG789550.2211789202020184No Hit
GGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG769270.21549782527237882No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG759530.21276933096198977No Hit
GGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC752060.2106767383029953No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA718320.20122505472676064No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA693990.1944094216085096No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG693280.19421052725939497No Hit
CCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG685340.19198627214394437No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC684720.1918125897545767No Hit
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA679900.19046234924368602Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC657500.18418737259556342No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG657370.18415095532037343No Hit
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA655080.18350945101125732Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG654180.18325733141378808No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACG653520.18307244370897735No Hit
CCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC643860.18036636002947445No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT625820.17531276276464713No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG605810.16970730371424833No Hit
CCCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT597310.16732617418259466No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC596800.16718330641069543No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG580670.16266476295827498No Hit
CCGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG563820.15794452382787919No Hit
GCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG518710.1453077293369501No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG518300.14519287485365856No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA517820.14505841106834166No Hit
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA517580.14499117917568322Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA515650.1444505227055548No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG503570.1410665174417458No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT471550.1320966624295634No Hit
GGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAAA461830.1293737707768959No Hit
CGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA459360.12868184254828594No Hit
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC458480.12843532560853826Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG455060.12747727113815527No Hit
GCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA439720.12318003266573559No Hit
CCGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT436790.12235924330953026No Hit
CCGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAA435420.1219754612556049No Hit
GGGCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAAAAAAAAAAAAAAA426390.11944586129433046No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA423260.11856904536090976No Hit
GGGGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAA413420.11581253776191305No Hit
GGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGAT412580.11557722613760844No Hit
GGCCCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA408650.1144763038953262No Hit
GGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT396820.11116233185303646No Hit
GGCCCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA395270.11072812587961725No Hit
GGGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT393770.11030792655050191No Hit
GGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC390590.10941710397277735No Hit
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA386760.10834419501910282Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG380150.1064925166421345No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA377680.10580058841352454No Hit
CGGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG377340.1057053432322584No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAGATCGG374420.10488735520491385No Hit
CCGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG374110.10480051401023001No Hit
CCGCGCGCCCCATTGAATACCCCAAAAAAAAAAAAAAAGATCGGAAGAGC370760.10386206884187238No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG368360.10318974991528783No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAAAAAAAAAAAA363000.10168823764591561No Hit
GGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT358230.10035200377932879No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)