FastQCFastQC Report
Mon 25 Sept 2023
SRR22969817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR22969817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35723464
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG6425641.798716944135093No Hit
GGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC5588701.5644339529895535No Hit
GGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC4724381.3224865315412861No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG3738471.0465026571891236No Hit
CCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC2971080.8316886626672038No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG2857440.7998776378460946No Hit
CCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC2597910.7272279082454042No Hit
GGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC2308410.6461887346647011No Hit
GGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC2251760.6303308100244702No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG2207070.617820824990544No Hit
GCGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1875470.5249966800531998No Hit
GCGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1845200.5165232576549686No Hit
CGCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1833570.5132676943087042No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1800990.5041476380901919No Hit
GGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA1761810.49318005667087605No Hit
CCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1751580.4903163926096305No Hit
CGCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1720680.4816666155331409No Hit
GCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1663870.4657639024031936No Hit
GGGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAA1615660.4522685705955055No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1599590.44777012665960947No Hit
CGGTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1561600.43713565963255974No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT1559630.4365842013529259No Hit
CGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1555350.4353861092530108No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG1517500.42479083215446295No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1457570.40801474347504485No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1419200.3972739037849185No Hit
GGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT1415680.39628855701115656No Hit
GCCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC1324960.370893483341929No Hit
GGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1252520.3506154946228059No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1185520.33186031455404214No Hit
CCCTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAGC1150360.3220180439388521No Hit
GGGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG1123810.3145859539265285No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG1109520.310585781938728No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA1105850.3095584459558569No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG1093000.30596137037550447No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG1087740.30448894877607613No Hit
GGGTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG1008490.2823046499633966No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA974480.27278429661804354No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG948050.2653857979730073No Hit
CCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG928450.25989920798274213No Hit
GGGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG919350.25735186263011894No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC915090.25615936909141845No Hit
GGGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA889710.24905479491014645No Hit
CCCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT888350.24867409274755664No Hit
CCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA875470.24506861932538232No Hit
CGGCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCC861070.2410376552509018No Hit
GGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGT845860.23677994944723166No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG845200.2365951969271513No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG813380.22768788603479215No Hit
CGGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG795770.2227583528853753No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCG789790.22108438308222292No Hit
GGGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG750190.20999923187740135No Hit
GGGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA748470.2095177556129495No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT748100.20941418223048022No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG740950.2074126965962763No Hit
GCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG733490.2053244332632468No Hit
GCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG706300.19771318929205747No Hit
GGGGGAGCGTTTACTTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACG674280.18874989278755272No Hit
CCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG637960.17858290562191842No Hit
GGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAAA633020.17720006100192298No Hit
GGGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT629890.17632388617184494No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG624970.17494664011306407No Hit
GCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA606160.1696811932907738No Hit
CCGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAA591140.165476673818642No Hit
GGGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG571570.1599984816701986No Hit
GGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGATC568000.1589991384934003No Hit
GGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT555850.15559801255555733No Hit
GGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA542850.1519589477660957No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG539630.15105757941055212No Hit
CGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGT525900.1472141671367592No Hit
CGGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA519070.1453022584819882No Hit
CCGACCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAG514000.14388302321409815No Hit
CGCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA505520.14150923325912626No Hit
GCGGGAGCGAAAGCGTTTGCCAAGAACGTTTTCATTAATCAAAAAAAAAA501050.14025795482767292No Hit
GGGGGAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGGTCAGGGGGCG494540.13843562315233485No Hit
CGGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT488900.13685682888982995No Hit
GCGGAAGGCAGCAGGCGCGCAAATTACCCATTTCCGACACGGAGAGGTAG487900.13657690082910212No Hit
CGCCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT484330.1355775576523038No Hit
CCGACCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGA481190.13469858354161848No Hit
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA479390.1341947130323084Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAGCGTTTACTTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC478030.13381401086971856No Hit
GGGGGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAA468770.13122187702737897No Hit
GGCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA460530.12891526980698176No Hit
CCCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA449370.12579127264925932No Hit
GGGTTACTTGGATAACTGTGGCAATTCCAGAGCTAATACATGCCAACGAG442390.12383737478537915No Hit
GGGGGCCGGGGACGCGTGCATTTATCAGATCCAAAAAAAAAAAAAAAGAT437970.12260009275696221No Hit
GGGCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCAAAAAAAAAAAAAAA437850.12256650138967487No Hit
CGGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCG430650.12055101935243458No Hit
GGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG424170.11873708551891832No Hit
GGGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCA422960.11839837256543767No Hit
CGGGCCGCCGGTGAAATACCACTACTCTTATCGTTTCCTCACTTACCCGG422400.11824161285143009No Hit
GGCTGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG419450.11741582507228303No Hit
CCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA410360.11487127900026717No Hit
CGCCCCTGGTGGAGCGCCGAGAAGACGATCGAACTCGGTCGTTTAGAGGA408160.11425543726666597No Hit
CCGCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAAAAAAAAAAAA401690.112444302713757No Hit
CGGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAA398410.11152613867456976No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACG397390.11124061205262736No Hit
CCGCAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGAT397380.11123781277202009No Hit
CGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAGA395410.1106863544923863No Hit
CGGCGCGCCCCATTGAATACCCCAGCTAGGAAAAAAAAAAAAAAAGATCG391500.10959183577494051No Hit
CCGCGGCGGCGTCCGGTGAGCTCTCGTCGGCCCTTGAAAATCCGGGGGAG384130.10752876596737651No Hit
GGCCCCGGGGACGCGTGCATTTATCAAAAAAAAAAAAAAAGATCGGAAGA375060.1049898184565752No Hit
CCGGGAGCGTTTACTTTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACAC373940.10467629902856006No Hit
GGGGAGCGTTTACTTTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTC373260.10448594794726515No Hit
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA371350.10395128535127501Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG371290.10393448966763133No Hit
GCCCGCGCCCCATTGAATACCCCAGCTAGGAATAATGGAATAGGACCCCA369950.10355938606625606No Hit
GGCCCCGGGGACGCGTGCATTTATCAGAAAAAAAAAAAAAAAGATCGGAA368580.10317588462305895No Hit
GCGGGAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCAAAA364140.10193300403342745No Hit
CCGCGCCCCCGCTCGACCGAAAGGGAGTCGGGTCCAGATCCCCGAGCCCG363750.10182383208974359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)