FastQCFastQC Report
Mon 25 Sept 2023
SRR23110902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23110902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30833845
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGA15824205.132087808056374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAA14637744.747296355676692Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTG10637343.4498908585679144Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAAC9064402.9397566213360675Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTC4814001.5612713886315508No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTG3589091.1640098729172441Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACT3571971.158457532623648Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA3546941.1503398294958025Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC3515271.1400686485905343Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATG3186231.0333547437888464TruSeq Adapter, Index 7 (97% over 35bp)
ATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGAA3173781.0293169729561786Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACT3112521.0094491945457986Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC2939490.9533322879452757Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2704080.876984365718904No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCT2341310.7593311829906391Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACAC2289370.7424860571232683No Hit
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGA2239200.7262149757839154No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCT2196840.7124768253845734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCT2161170.7009083687097734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT1997600.6478595193041932No Hit
GATGTCGGCTCTTCCTATCATTGTGAGATCGGAAGAGCACACGTCTGAAC1957130.634734331705955Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTC1736240.5630955205229837Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT1695270.549808173453554Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT1674140.5429553142010022Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAAC1607850.521456211510436Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTG1576390.5112531375830682Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCCCAAGATGAGTGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCC1417300.4596572370393637Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCTGAACT1351720.4383884007978895Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTG1334910.432936599376432Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTC1284670.4166428157111123No Hit
CCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG1255740.4072602687079733Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAA1212220.39314590833546703Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACTC1151640.3734986668059076Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACACGT1078710.3498460863379186No Hit
GCCAAGGATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG1076570.3491520438012191Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTCTGAAC1059510.34361916264416587Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA1055480.34231215730636255Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGGCTCCCAAA1049820.340476512092475No Hit
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAG1015040.3291966992763958No Hit
ATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAAC997290.3234400380490983Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAG988330.320534140325347Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG978860.31746283994098046No Hit
TCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCA911480.2956102296032168Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGA889290.28841359227173907Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTTTTTGGTCCCAAGGCTCGCAGATCGGAAGAGCACACGTCTGAACTC879360.2851931051738763Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTC862630.2797672492678095No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGA850920.275969474452505Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTTTTTGGTCCCAAGGCTCAGATCGGAAGAGCACACGTCTGAACTCCA768270.2491645138645537Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTG767110.24878830389138948Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGA763290.2475494055314866Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACTC719480.2333409926656893Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTG719000.23318531957334546Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGCGGATTACGGTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA702010.22767514074225906Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT683100.22154226954179734No Hit
CGGGTGCACCGCCGACCGACCTTGAGATCGGAAGAGCACACGTCTGAACT665180.21573047409429477Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACGTCTGAACTCC661620.21457589865941143Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGTCGGCTCTTCCTATCATTGTGAAAAGATCGGAAGAGCACACGTCTG645590.20937706601301265Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACAC643280.208627889256108No Hit
TCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACA639900.20753169123085363No Hit
CTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGTC633030.2053036200966827No Hit
GGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG624960.20268636623165226Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACAC592920.19229518731770234No Hit
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGAG592350.19211032552054408No Hit
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGC585860.19000549558447868No Hit
GGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAA576690.18703149088282697Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGA575290.18657744436349083Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGGTGTCCTAGGCGTAGAGGAACAACACCAGATCGGAAGAGCACACGT558480.18112564294203332No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTC554690.17989647415040194No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACA550030.17838514787889737No Hit
CGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC542550.17595924218987286Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTGTTTTTGGTCCCAAGGCTCGGCGGGTGCACCGCCGACCGACCTTGAGA540430.17527168603202098No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGACGTCGCTGCCGTGA536560.17401657172499893No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAG530910.17218416970053524No Hit
TTCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCC530790.1721452514274493Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGT527060.17093554177236087No Hit
GATGTCGGCTCTTCCTATCATTGTAGATCGGAAGAGCACACGTCTGAACT475630.1542558185656054Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTACGATAGGTGTCAAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCA474920.15402555211651353Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCAC467440.15159964642748902No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACG464920.15078236269268397No Hit
TCTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGT458380.14866131680949943No Hit
CGGGTGCACCGCCGACCGACCTTGGCGGGTGCACCGCCGACCGACCTTGA446710.14487651475189034No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATGCCGTC440770.1429500602341356RNA PCR Primer, Index 43 (100% over 34bp)
CTGTTTTTGGTCCCAAGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC439500.1425381751773092Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACT427550.1386625638158329Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAACT405850.13162484276612274Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCAC395660.12832003274324044No Hit
ACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACA391690.1270324865419801No Hit
GATGTCGGCTCTTCCTATCATAGATCGGAAGAGCACACGTCTGAACTCCA382040.12390280874798455Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCTGTTTTTGGTCCCAAGGCTAGATCGGAAGAGCACACGTCTGAACTC370360.12011476350095164Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACAC362470.1175558870455501No Hit
GATGTCGGCTCTTCCTATCATTGAGATCGGAAGAGCACACGTCTGAACTC361480.11723481129259099Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACACG353100.1145170185554218No Hit
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACA348270.11295055806371214No Hit
TGCCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCC345430.11202949226734454Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACGTCTGAACTCCA335900.10893873274643498Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTTCGAATTGTAGTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGT332300.10777118455385633Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC332300.10777118455385633Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACCCCAAGATGAGTGCTCTCTAGATCGGAAGAGCACACGTCTGAACTCC330430.10716470813160019Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA325800.1056631114283671Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTCTGAAC323850.10503068949072035Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTGAACTCCAG323390.10488150277722418Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTACGATAGGTGTCAAGTGGAAAGATCGGAAGAGCACACGTCTGAACTCC323070.10477772071566165Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTTCGAATTGTAGTCTGGAGAAGCGAGATCGGAAGAGCACACGTCTGAA313410.10164479973224227Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTCGCTGCCGTGATCGTGGTAAGATCGGAAGAGCACACGTCTGAAC313200.10157669275434186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGACGTCGCTGCCGTG310830.10080805686089424No Hit
AGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTCTGA309710.10044481964542534Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)