FastQCFastQC Report
Mon 25 Sept 2023
SRR23110903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23110903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32287393
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAA20276396.279971256892744Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGA20124346.232878572760582Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTG13003374.0273830717766534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAAC9595042.971760525849826Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTC5762241.7846718067327394No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTG4697611.4549362966530002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC3946461.2222913135167028Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGAA3864951.1970461659756797Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACT3082890.9548277868083063Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATGTCGGCTCTTCCTATCATTGTGAGATCGGAAGAGCACACGTCTGAAC2742700.8494646811527954Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACT2714990.8408823840314392Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCT2664700.8253066452283714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA2648150.8201808055546634Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACAC2490070.7712205194144972No Hit
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGA2460330.7620094939222872No Hit
TCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCT2450700.7590269056408487Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC2447030.7578902390787636Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCT2231380.6910994641159167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2160430.6691249429769693No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT1953740.6051092449613383Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT1706740.5286087978673286No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTG1610470.49879220660522205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTG1560000.4831607184884825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAAC1557960.482528892933536Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTC1513870.46887340826805063No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACA1503620.46569879457285385No Hit
TTCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTC1369650.42420581928060896Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATG1308380.4052293723435646TruSeq Adapter, Index 6 (97% over 36bp)
GAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAA1299920.4026091546009924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAAC1275790.39513564938488527Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGA1210240.3748336076560904Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT1168160.3618006569932729Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTCTGAAC1067200.330531486391608Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGTGCACCGCCGACCGACCTTGAGATCGGAAGAGCACACGTCTGAACT1020100.3159437493141673Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCAAGGATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG1019140.3156464196412513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAG982280.3042301990749145No Hit
CTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACTC957600.2965863487336992Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTC956070.2961124795674894No Hit
CTGTTTTTGGTCCCAAGGCTCGCAGATCGGAAGAGCACACGTCTGAACTC948090.29364092666137526Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACA935160.289636267629288No Hit
CCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG909690.28174773974473566Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGA886410.2745374951765229Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAG875260.2710841349129674No Hit
GCCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA869530.2693094484277501Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTG864490.26774846764494115Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGA861770.2669060335716792Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAG825040.25553007639854974Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCA823770.25513673401875464Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG821350.2543872154682789No Hit
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACAC820950.25426332810456387No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGACGTCGCTGCCGTGA785240.24320328370890767No Hit
ATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACGTCTGAACTCC782130.24224005945602362Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGGCTCCCAAA756560.2343205597305425No Hit
GATGTCGGCTCTTCCTATCATTGTGAAAAGATCGGAAGAGCACACGTCTG755560.23401084132125502Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTTTTTGGTCCCAAGGCTCAGATCGGAAGAGCACACGTCTGAACTCCA748360.23178086877438508Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTG732150.2267603333598349Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGCGGATTACGGTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA723010.22392950709894727Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACACGT720330.2230994617620568No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACAC706630.21885631955481819No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACAC685210.21222215122788018No Hit
GATGTCGGCTCTTCCTATCATTGTAGATCGGAAGAGCACACGTCTGAACT653070.20226780155338028Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACTC645630.1999634965882814Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTC639070.19793174382335543No Hit
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGAG638540.19776759306643307No Hit
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGC633270.196135377049488No Hit
CTCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCAC617500.19125111773502432No Hit
CGAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCTGAACT608260.18838931963320793Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGT600000.18583104557249325No Hit
GGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAA579730.17955305341623587Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGA568640.1761182762572376Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGTGCACCGCCGACCGACCTTGGCGGGTGCACCGCCGACCGACCTTGA563200.17443340811071367No Hit
CTGTTTTTGGTCCCAAGGCTCGGCGGGTGCACCGCCGACCGACCTTGAGA561010.17375512479437408No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT547190.169474816378021No Hit
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACAC513880.15915809616465473No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGACGTCGCTGCCGTG500640.15505742442568837No Hit
GATGTCGGCTCTTCCTATCATTGAGATCGGAAGAGCACACGTCTGAACTC496870.15388978602267456Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTACGATAGGTGTCAAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCA491980.15237526300125873Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCTGTTTTTGGTCCCAAGGCTAGATCGGAAGAGCACACGTCTGAACTC485910.15049527225688367Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACG478020.1480515940076054No Hit
CACCCCAAGATGAGTGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCC477920.14802062216667664Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACGTCTGA471120.14591453698352172Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG447160.13849368389699349Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGTTTTTGGTCCCAAGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC446640.13833263032416399Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGTC442960.13719286657798602No Hit
TCTGGTGTCCTAGGCGTAGAGGAACAACACCAGATCGGAAGAGCACACGT442680.1371061454233855No Hit
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCAC433390.13422886140110477No Hit
GATGTCGGCTCTTCCTATCATAGATCGGAAGAGCACACGTCTGAACTCCA425330.1317325310222476Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAACT425110.13166439297220434Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCC418060.12948087818672754Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACT410870.1272540028239505Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACGTCTGAACTCCA380460.11783546599751797Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGACGTCGCTGCCGTGAT370300.11468872695915709No Hit
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACG366400.11348082516293588No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGC362060.1121366472666282No Hit
CCCCTGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAAC361180.1118640950664552Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA357010.11057256929972636Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATTGTGGAGACGTCGCTGC356830.11051681998605462No Hit
TCTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGT355120.10998720150617301No Hit
CTCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGACGTCGCTGCCGTG353440.10946687457857003No Hit
CGATGTCGGCTCTTCCTATCATTGTGAGATCGGAAGAGCACACGTCTGAA350400.10852533061433607Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCAC347950.10776652051158171No Hit
ACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACA331070.1025384737628089No Hit
AGACGTCGCTGCCGTGATCGTGGTCTGAGATCGGAAGAGCACACGTCTGA330120.10224424127398578Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCT326880.10124075362789432Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGT325560.10083192532763484No Hit
CCCCTGTTTTTGGTCCCAAGGCTCAGATCGGAAGAGCACACGTCTGAACT323220.10010718424990211Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)