FastQCFastQC Report
Mon 25 Sept 2023
SRR23110904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23110904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25877071
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAAC7319602.8286045201947316Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC4598191.7769360373127236Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGA4301491.662278547676435Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAA4138111.5991415720890512Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACT4116761.5908910247222339Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTG3623411.4002396175363123Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTC3510521.3566141237545781No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC3495731.3508986391852462No Hit
AGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACT3138821.2129734466470337Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGA2977891.1507832551837107No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACAC2598501.0041708352541134No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG2553840.9869123132212297TruSeq Adapter, Index 10 (97% over 36bp)
CGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA2506450.9685988031643922Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC2225810.8601475800719486Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT2164300.8363775019205226No Hit
CGAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCTGAACT2125560.8214067194853699Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCT1990830.7693413215120057Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTG1933020.7470010806091617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACTC1597910.617500334562594Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTG1392400.5380825364663566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTC1356110.5240585381552649Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAG1350040.5217128321825912No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT1258070.4861717154928392Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG1169670.45201019852671886No Hit
GAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAAC1155380.44648793520719554Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA1090500.42141554583206114Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGCCGTC1007620.3893871914638252RNA PCR Primer, Index 47 (100% over 34bp)
AGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACACGT956550.36965157300839807No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTG934440.3611073293418718Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCT913840.35314661384976687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT907350.35063860202725416No Hit
AGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACTC858800.33187681867086116Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTACGATAGGTGTCAAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCA839940.32458851312808934Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACGCGGATTACGGTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA813340.3143091426382839Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGTTTTTGGTCCCAAGGCTCGCAGATCGGAAGAGCACACGTCTGAACTC809700.31290249193967895Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGTTTTTGGTCCCAAGGCTCAGATCGGAAGAGCACACGTCTGAACTCCA799480.3089530495935958Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCT791710.3059503913715737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTCTGAAC786640.3039911278985168Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAA752480.29079025211160875Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACCCCAAGATGAGTGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCC745000.2878996622144755Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGTC733110.2833048608940324No Hit
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGAG725060.2801939987721176No Hit
CGGGTGCACCGCCGACCGACCTTGAGATCGGAAGAGCACACGTCTGAACT719910.2782038198990914Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGA715390.27645709980082367Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGA697490.26953977905768395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGGTGTCCTAGGCGTAGAGGAACAACACCAGATCGGAAGAGCACACGT660760.25534574604676086No Hit
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCAC658210.25436031767273815No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTG657890.2542366560728608Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACAC646460.2498196183022414No Hit
GCCAAGGATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG611920.23647189436547902Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGAGGTAGTGACAATAAATAACAAGATCGGAAGAGCACACGTCTGAA597060.23072935882117418Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAG568160.21956117058225022Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCA565560.21855642008324666Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGC555920.21483111438694125No Hit
GCGGCGGGTAGAATCCTTTGCAGACGACTTAAATACGCGACGGGGTATTG535020.20675446614495124No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGACGTCGCTGCCGTGA519970.20093850652571923No Hit
GATGTCGGCTCTTCCTATCATTGTGAGATCGGAAGAGCACACGTCTGAAC505210.19523461523137609Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGTTTTTGGTCCCAAGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC504060.19479020635681682Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGA483760.18694542361459687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACA478600.1849513803165745No Hit
TCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTC473220.18287231966863637No Hit
TGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAG470660.18188302686961752No Hit
CTGTTTTTGGTCCCAAGGCTCGGCGGGTGCACCGCCGACCGACCTTGAGA465290.17980783064667558No Hit
TCTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGT449920.1738682094275662No Hit
GGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG421930.1630516838632935Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAA416610.1609958097653324Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAG414370.16013017856619088No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCG413720.15987899094144TruSeq Adapter, Index 10 (97% over 36bp)
GAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACT408760.15796223614334096Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACA408440.1578385745434636No Hit
ATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGAA399690.1544572026718171Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTTCGAATTGTAGTCTGGAGAAGCGAGATCGGAAGAGCACACGTCTGAA399290.15430262567197037Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGA390840.15103718655020887No Hit
CGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTCTGAAC389840.15065074405059212Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCAC388730.15022179287601756No Hit
ACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACA385410.14893880377728996No Hit
AAGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACACG379680.14672448825448597No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTC377050.14570814448049393No Hit
GACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAACT371540.14357884630760567Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAGC368570.14243111208374393No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGAGATCGGAAGAGCACACG366140.14149205680967525No Hit
CGGGTGCACCGCCGACCGACCTTGGCGGGTGCACCGCCGACCGACCTTGA359080.13876377276238103No Hit
TCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACA357360.13809909166304024No Hit
CCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG357100.13799861661313986Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTACGATAGGTGTCAAGTGGAAAGATCGGAAGAGCACACGTCTGAACTCC356720.1378517684632855Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTC349540.13507711131603728No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGA346710.13398347904212188Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTATCGTCACCGCAGTAGAGTTTAACCACCAAGATCGGAAGAGCACACGT338430.13078373514529523No Hit
TGCCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCC327850.12669517349935006Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCC325650.12584500000019322Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACGTCTGAACTCC320860.123993940427029Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACTCGACGGATCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA319450.12344905650256939Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTG316860.12244817042856201Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTGTTTTTGGTCCCAAGGCTAGATCGGAAGAGCACACGTCTGAACTC315180.1217989470292059Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCACA312850.12089853600509887No Hit
GCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT311670.12044253385555112Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCTGAACTC309650.11966192000632529Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACAC308010.11902815430695382No Hit
CCGACGCGGATTACGGTGGCGAGATCGGAAGAGCACACGTCTGAACTCCA304370.11762150360834889Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACAC302220.11679065223417287No Hit
GGGAAGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCAC301850.11664766850931468No Hit
TCTGATGATTCATGATAACTCGATAGATCGGAAGAGCACACGTCTGAACT298250.1152564755106944Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGACGTCGCTGCCGTGATCGTGGCAGATCGGAAGAGCACACGTCTGAACT296560.1146033876863421Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACAC285680.1103988932905119No Hit
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACA283500.10955644864134739No Hit
GCCCATTAAAGCGGTACGTGAGCTGGAGATCGGAAGAGCACACGTCTGAA282300.10909271764180731Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACTC278170.10749671011839014Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC274970.10626009411961654Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGTAGATCGGAAGAG266620.10303329924781672No Hit
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACG264570.1022410921236024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)