FastQCFastQC Report
Mon 25 Sept 2023
SRR23110905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23110905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24857738
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAAC6461032.599202711043137Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACT4254641.7115958016775301Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTG4129811.6613780385005266Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGA4116481.6560155232145417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAA3876501.5594741564980692Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC3868521.556263888532416Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGA3563031.4333685550953994No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC3375951.3581082880509883No Hit
CGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA3295671.3258125095694548Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTC3190681.2835761644925214No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACAC3030681.2192098894919563No Hit
TCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC2658701.069566345900017Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACT2417990.9727313080538542Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT2265160.9112494467517518No Hit
CGAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCTGAACT1948280.7837720391131324Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTC1621160.6521751898744769Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG1556460.6261470774211233No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTG1538880.6190748329554362Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACTC1538500.61892196305231Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCT1452850.5844658914660699Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATG1399740.5631003110580697TruSeq Adapter, Index 3 (97% over 36bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAG1371420.5517074803829697No Hit
AGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACACGT1353090.5443335190032175No Hit
GCCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA1270780.5112210934076142Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTG1169320.4704048292728807Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT1135240.45669481269776036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT1100000.4425181406288859No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTG972270.3911337387174972Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAAC950660.38244026870023334Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAA853700.3434343060498908Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGAG838520.33732755570921213No Hit
CTGTTTTTGGTCCCAAGGCTCGCAGATCGGAAGAGCACACGTCTGAACTC834540.3357264446185731Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCT800630.3220848172106408Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTC799650.32169057377626237RNA PCR Primer, Index 45 (100% over 34bp)
CTGTTTTTGGTCCCAAGGCTCAGATCGGAAGAGCACACGTCTGAACTCCA790390.3179653756106046Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCT789230.31749872011685054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCCCAAGATGAGTGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCC772430.3107402612417912Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGTGCACCGCCGACCGACCTTGAGATCGGAAGAGCACACGTCTGAACT749480.3015077236713976Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGGTGTCCTAGGCGTAGAGGAACAACACCAGATCGGAAGAGCACACGT714860.2875804709181503No Hit
CGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAG672480.27053145382737565Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCA628500.2528387739865952Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACA628490.25283475109440773No Hit
CGACGCGGATTACGGTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA614380.24715845021779534Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCAAGGATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG612570.24643030673185146Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTGTTTTTGGTCCCAAGGCTAGATCGGAAGAGCACACGTCTGAACTC592310.2382799271599049Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGTTTTTGGTCCCAAGGCTCGGCGGGTGCACCGCCGACCGACCTTGAGA590810.2376764933317746No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGA571580.22994047165514417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACAC570290.2294215185629521No Hit
AGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACTC563600.22673020368949096Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCAC554720.2231578754269596No Hit
GGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG552890.22242168615664065Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGACGTCGCTGCCGTGA546420.21981887491130525No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGA543810.21876890005035854Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGA504340.20289054458615666Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACACG485680.1953838277642157No Hit
GGAAGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACA479000.1926965357829421No Hit
AGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTCTGAAC476840.19182759107043448Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATGTCGGCTCTTCCTATCATTGTGAGATCGGAAGAGCACACGTCTGAAC473380.19043567037354728Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCAC469940.1890517954610351No Hit
CTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGTC463980.18665415171726407No Hit
TCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTC447430.1799962651468931No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTG444980.17901065656094692Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGTGCACCGCCGACCGACCTTGGCGGGTGCACCGCCGACCGACCTTGA435940.17537396202341501No Hit
TGCCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCC428630.17243322783432666Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGC424050.1705907432124355No Hit
ACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACA416270.167460933090533No Hit
CGAGGCAAACTCTGAATACTAGATAAGATCGGAAGAGCACACGTCTGAAC414890.16690577396865314Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGAGGTAGTGACAATAAATAACAAGATCGGAAGAGCACACGTCTGAA414800.16686956793896532Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCA404400.1626857600639286TruSeq Adapter, Index 3 (97% over 36bp)
ATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGAA400770.16122545019985327Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACGATAGGTGTCAAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCA395920.15927434748889863Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAAGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCAC372720.14994123761381667No Hit
TTCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCC364790.14675108410910115Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAGC355490.1430097943746933No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAG335640.13502435338243568No Hit
CTGTTTTTGGTCCCAAGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC328830.13228476380272414Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACAC328750.13225258066522386No Hit
GGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAA324150.1304020502589576Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG324000.13034170687614455Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACTCGGATAAC322790.12985493692145278No Hit
GAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACT321260.1292394344167599Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTCGACGGATCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA320880.12908656451363354Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACAC320280.12884519098238142No Hit
GACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAACT318150.1279883149464364Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACA317390.12768257514018372No Hit
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACA315460.1269061569479894No Hit
TCTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGT311680.12538550370110105No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGAGATCGGAAGAGCACACG309190.12438380354640474No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTC305330.12283096716201611No Hit
TTACGATAGGTGTCAAGTGGAAAGATCGGAAGAGCACACGTCTGAACTCC298970.12027240773074363Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCA297440.11965690522605073No Hit
GCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT295880.11902933404479522Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTTCGAATTGTAGTCTGGAGAAGCGAGATCGGAAGAGCACACGTCTGAA295560.11890060149479409Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGA288790.11617710348383267Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACAC285640.11490989244475906No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGACGTCGCTGCCGTG284950.1146323128838191No Hit
GCCCATTAAAGCGGTACGTGAGCTGGAGATCGGAAGAGCACACGTCTGAA283960.11423404655725312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCAC277520.11164330398848035No Hit
CCCCTGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAAC273540.11004219289784131Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACGTCTGAACTCC271680.10929393495095972Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTG266840.10734685513219264Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGCGGATTACGGTGGCGAGATCGGAAGAGCACACGTCTGAACTCCA262300.10552046207905161Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGGATTACGGTGGCGGCCTAAGCCCGGGCTAGATCGGAAGAGCACACG254240.10227801097589813No Hit
CTCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGACGTCGCTGCCGTG251760.10128033371338938No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)