FastQCFastQC Report
Mon 25 Sept 2023
SRR23110906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23110906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29446028
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAA26194828.895875532007237Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGA13840144.70017212508254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC8270732.8087761106523432Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAAC7908382.6857204645733543Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTG6196612.1043958798110225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTG5346941.8158442286341643Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATG5143371.7467109655672406TruSeq Adapter, Index 2 (97% over 37bp)
AGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACT3432071.1655459948621933Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACT3356651.139933032733651Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2992051.0161132768059582No Hit
TCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCT2978621.0115523900201413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGTCGGCTCTTCCTATCATTGTGAGATCGGAAGAGCACACGTCTGAAC2915910.9902557995258308Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTC2813310.9554123904249496No Hit
ATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGAA2667610.9059320326666809Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTG2004640.6807845187133559Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCT1878710.6380181394923621Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT1855370.6300917733284774No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCT1650950.5606698465409324Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC1624230.5515956175821065Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACAC1590930.5402867918212941No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGA1506440.5115936179915335Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACCCCAAGATGAGTGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCC1470130.4992625830553445Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGCCGTC1375050.4669729988710192RNA PCR Primer, Index 39 (100% over 34bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGA1367610.46444634230463955No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAG1360140.4619094976069438No Hit
GAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAAC1310090.4449122985280052Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACTC1300970.4418151066079269Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAAC1224950.4159983818530635Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTC1220730.4145652513812729No Hit
CGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA1214750.41253441720560746Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACTC1202890.4085067092919969Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTG1106060.3756228174475688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTC1096390.37233884312002963No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT1064800.361610740844232Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA1034690.3513852530466927Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCAAGGATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG950450.3227769803112325Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACGATAGGTGTCAAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCA950240.3227056633920201Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGTTTTTGGTCCCAAGGCTCAGATCGGAAGAGCACACGTCTGAACTCCA883070.2998944373753907Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACGTCTGAACTCC863500.29324837971355594Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTC850910.2889727606045882Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACA826770.28077471093894224No Hit
GTATCGTCACCGCAGTAGAGTTTAACCACCAAGATCGGAAGAGCACACGT816870.2774126276046467No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGC808520.2745769310550136No Hit
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGC806800.2739928115262269No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTG762710.2590196545354097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGA754010.25606509645375597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTTTTTGGTCCCAAGGCTCGCAGATCGGAAGAGCACACGTCTGAACTC741070.2516706158127677Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT739220.25104234771494477Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCAC736250.2500337227146561No Hit
CGGGTGCACCGCCGACCGACCTTGAGATCGGAAGAGCACACGTCTGAACT734720.2495141280175377Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGTTTTTGGTCCCAAGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC720590.2447155181676795Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG715750.24307183298202395Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGA714420.24262015916034582Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA708390.24057234476582037Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACAC701100.23809662885602093No Hit
AGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTCTGAAC697180.23676537969739078Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACGCGGATTACGGTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA694050.23570241799674985Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAG658010.22346307624240527No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTG637680.21655891925389734Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCTGAACT618570.2100690796055753Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGAGCACACGTCTGAA564540.19172025510537447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAA546760.18568208927873056Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGTCGGCTCTTCCTATCATTGAGATCGGAAGAGCACACGTCTGAACTC546520.1856005842282022Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATGTCGGCTCTTCCTATCATTGTAGATCGGAAGAGCACACGTCTGAACT541340.18384143355429805Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG540600.18359012631516888No Hit
TCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACGTCTGAACTCCA504520.17133720038573624Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACG495910.16841320669803073No Hit
TCTGGTGTCCTAGGCGTAGAGGAACAACACCAGATCGGAAGAGCACACGT493680.16765588893687122No Hit
GGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAA493330.16753702740485066Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGTC493220.1674996709233585No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT487820.16566580728647No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGT473910.16094191039959618No Hit
GAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACT471880.16025251351387698Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACAC468420.15907748236875954No Hit
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACAC463930.1575526587151245No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCA463360.1573590842201196No Hit
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGAG456120.15490034852918025No Hit
GACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAACT453790.15410907033030058Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCA450960.15314798994282014Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGATGTCGGCTCTTCCTATCATTGTGAGATCGGAAGAGCACACGTCTGAA448250.15222766208060387Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACA444580.1509813140162741No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCT428020.1453574655298161Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACG418460.1421108476837691No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGT415920.1412482525656771No Hit
TCCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACAC410430.1393838245348405No Hit
ACTTTAAATAGGTAGGACGTGAGATCGGAAGAGCACACGTCTGAACTCCA400500.1360115530692289Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGGCTCCCAAA393600.13366828286653806No Hit
CGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCACA382130.12977302066003604No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGA377070.12805462251139613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTCGAATTGTAGTCTGGAGAAGCGAGATCGGAAGAGCACACGTCTGAA372540.126516214682673Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACTC370110.12569097604607318Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC365850.12424426139919448Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATGTCGGCTCTTCCTATCATTGTGAAAAGATCGGAAGAGCACACGTCTG358750.12183307032106333Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACACGT356180.1209602870716553No Hit
TTACGATAGGTGTCAAGTGGAAAGATCGGAAGAGCACACGTCTGAACTCC351660.1194252752867042Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACA349950.11884455180168953No Hit
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACAC347220.11791743185192924No Hit
CGCGACGGGGTATTGTAAGTGGCAGAGTGGCAGATCGGAAGAGCACACGT334550.11361464439278535No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGACGTCGCTGCCGTGA333630.11330220836575988No Hit
CTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTGAACTCCAG328780.11165512713633227Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACG321450.10916582705144476No Hit
TGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAA312740.10620787292601908Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAG309600.10514151518160617Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCATTAAAGCGGTACGTGAGCTGGAGATCGGAAGAGCACACGTCTGAA305840.10386460272332826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGTCGGCTCTTCCTATCATAGATCGGAAGAGCACACGTCTGAACTCCA305370.10370498866604351Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGT301720.10246543268925777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)