FastQCFastQC Report
Mon 25 Sept 2023
SRR23110907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23110907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37912738
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAA34342609.058327573176065Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGA16719924.4101061759243025Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTG8880312.3423024736435547Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTG7342981.9368107890282154Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC6669331.7591264445211Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAAC6444321.6997769984325586Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACT5182731.3670154869848756Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC4844161.277713047261319No Hit
TCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCT4413501.1641206182470913Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGTCGGCTCTTCCTATCATTGTGAGATCGGAAGAGCACACGTCTGAAC3651120.963032530122198Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACT3465550.9140859201464162Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGAA3394440.8953296910394601Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATG3193860.8424239895309065TruSeq Adapter, Index 7 (97% over 37bp)
TCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC2897910.7643631541462397Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT2860800.7545748872054558No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCT2478720.6537960935451299Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGA2284430.6025494650373181No Hit
CTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACTC2272680.5994502428181262Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACAC2175320.5737702193916989No Hit
CGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA2143950.5654959554754394Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGA2068550.5456081805539869Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTC1926010.5080113179902754No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTC1801950.47528880662747175No Hit
CGGGTGCACCGCCGACCGACCTTGAGATCGGAAGAGCACACGTCTGAACT1768240.46639733590330507Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTG1751890.4620848011557488Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAG1671240.440812267370402No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGCCGTC1611460.4250444797735262RNA PCR Primer, Index 37 (100% over 34bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTG1556420.4105269316080522Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTC1528180.4030782477382667No Hit
TTCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTC1526980.40276173142651955Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAAC1515460.39972317483374586Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACTC1375100.3627013169030419Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCTGAACT1345880.35499414471199625Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCAAGGATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG1240090.32709059419554454Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACGATAGGTGTCAAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCA1217990.3212614187875326Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA1119940.2953993984818506Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAG1099050.2898893770215172No Hit
TCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACA1075390.2836487304082338No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACAC1073380.28311856558605714No Hit
CCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG1069460.28208461230101606Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGC1054840.278228388569562No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCT1036160.2733012846500298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACGTCTGAACTCC1027650.27105665647255545Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACG1014410.26756442649961076No Hit
GAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAAC1001960.26428056976523295Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACA995310.26252654187096697No Hit
GCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT969210.2556423120904642Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCAC932080.24584876987781787No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACAC912100.24057877328722607No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGC907370.23933117149175562No Hit
CTGTTTTTGGTCCCAAGGCTCAGATCGGAAGAGCACACGTCTGAACTCCA890380.23484982804460072Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGTC852960.22497979438994886No Hit
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACAC823490.2172066813006225No Hit
TCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCA802100.2115647780437277Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGTCGGCTCTTCCTATCATTGTAGATCGGAAGAGCACACGTCTGAACT792600.20905902390906192Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGAG787320.207666352137374No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGACGTCGCTGCCGTGA774000.20415302107697947No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGA760130.20049462004036742Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT759370.20029415970959416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGTCGGCTCTTCCTATCATTGAGATCGGAAGAGCACACGTCTGAACTC749540.1977013635891979Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA732920.1933176126714984Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG713040.1880739924402189No Hit
TCTGGTGTCCTAGGCGTAGAGGAACAACACCAGATCGGAAGAGCACACGT710320.18735655546692512No Hit
CTGTTTTTGGTCCCAAGGCTCGCAGATCGGAAGAGCACACGTCTGAACTC705250.186019274049793Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTATCGTCACCGCAGTAGAGTTTAACCACCAAGATCGGAAGAGCACACGT700030.1846424280936924No Hit
GATGTCGGCTCTTCCTATCATAGATCGGAAGAGCACACGTCTGAACTCCA695750.1835135199151272Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACGTCTGAACTCCA693270.18285938620418288Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACGCGGATTACGGTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA676570.1784545341990336Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT666700.1758511875349124No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACG664350.17523134309107405No Hit
CGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACGTCTGA653550.17238269628534875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGACGTCGCTGCCGTGAT645490.1702567617247797No Hit
CTGTTTTTGGTCCCAAGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC639700.16872957052059917Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCT634780.16743185364243543Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGT633210.16701774480123277No Hit
CGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGAGCACACGTCTGAA630060.16618688948289623Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACACGT609280.16070588201780625No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGT593590.1565674312417109No Hit
CGATGTCGGCTCTTCCTATCATTGTGAGATCGGAAGAGCACACGTCTGAA593450.15653050433867371Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTCTGAAC590090.1556442586657814Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAG559110.14747286255083977Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACAC557990.14717744732654234No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCA527580.13915639645968064No Hit
GAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAA522130.13771888487716186Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGTCGGCTCTTCCTATCATTGTGAAAAGATCGGAAGAGCACACGTCTG510490.13464867665321348Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACGATAGGTGTCAAGTGGAAAGATCGGAAGAGCACACGTCTGAACTCC506120.13349602975126723Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGT504440.13305290691482108No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTG502260.13247790228181358Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACT501320.13222996450427824Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGAGGTAGTGACAATAAATAACAAGATCGGAAGAGCACACGTCTGAA483080.12741891656571994Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTGAACTCCAG477720.1260051437065822Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACG467490.12330684214893685No Hit
CGGGTGCACCGCCGACCGACCTTGGCGGGTGCACCGCCGACCGACCTTGA458770.12100682361690679No Hit
CTCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACA456940.12052413624149225No Hit
CGCGACGGGGTATTGTAAGTGGCAGAGTGGCAGATCGGAAGAGCACACGT454200.119801423996336No Hit
ATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACA443860.11707410844344716No Hit
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAGC439890.11602696697875No Hit
ACTTTAAATAGGTAGGACGTGAGATCGGAAGAGCACACGTCTGAACTCCA439180.11583969482763287Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGGATTACGGTGGCGGCCTACGGGTGCACCGCCGACCGACCTTGAGAT435170.11478200281921079No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTG430280.11349219884884074Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCATTAAAGCGGTACGTGAGCTGGAGATCGGAAGAGCACACGTCTGAA420770.11098380707824373Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCACA419100.1105433218777288No Hit
CTCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCAC410850.10836727223446643No Hit
CTGTTTTTGGTCCCAAGGCTCGGCGGGTGCACCGCCGACCGACCTTGAGA404280.1066343454276502No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGA387230.10213717616490796Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)