FastQCFastQC Report
Mon 25 Sept 2023
SRR23110908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23110908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27369933
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAAC9746953.561188841784889Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATG7550882.7588229755622713TruSeq Adapter, Index 9 (97% over 36bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTC5823512.1277034181998182No Hit
CTGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC5514952.014966569337236Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACT5144491.879613662189089Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCT4460341.6296495866467777Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACT4106091.500219236926886Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGA4000191.461527143672584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAA3071571.122242425657381Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTG3064581.1196885282839384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACAC2642210.9653695535169926No Hit
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2523120.9218583034163803No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTG2357870.8614818311758381Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT2249180.8217703711587455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGA2106300.7695671012420819No Hit
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT1936420.7074989916855112No Hit
TTACGATAGGTGTCAAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCA1868410.6826505567258787Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTG1804510.6593037695780987Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACTC1724390.6300307713577523Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAAC1647860.6020694314450825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAG1583250.578463235551216No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGA1582980.5783645871548169Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTG1421210.5192595831345295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCCGTC1364320.49847400064881414RNA PCR Primer, Index 35 (100% over 34bp)
AGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTCTGAAC1292900.4723796729791045Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC1285750.4697673172966847Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTG1255200.4586054339263454Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGA1065310.38922638210331023Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT1021250.3731284252687064Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACTC1011100.3694199762929635Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA937430.3425035786532616Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCTGAACT900040.3288426025741459Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACCCCAAGATGAGTGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCC898640.32833109237059516Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACGCGGATTACGGTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA896080.3273957594269595Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG876600.3202784603089821No Hit
CTGTTTTTGGTCCCAAGGCTCGCAGATCGGAAGAGCACACGTCTGAACTC875830.31999712969702926Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAA835170.30514141192819144Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGC834460.304882003182105No Hit
CCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG812730.29694263409413535Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGTTTTTGGTCCCAAGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC808150.2952692649996622Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCT796740.2911004568407237Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTTTTTGGTCCCAAGGCTCAGATCGGAAGAGCACACGTCTGAACTCCA767030.2802454795925149Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTC760530.27787061079031505No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCT698480.2551997478400842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCAC697390.25480150061017687No Hit
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGGCTCCCAAA688730.25163744463678445No Hit
CTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGTC687500.25118804638652203No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAG677210.2474284463904241No Hit
TTCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTC672230.24560893152350793Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAA629480.22998960209365513Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT623650.22785952746029742No Hit
GCCAAGGATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG605060.22106740268600586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACA589490.21537867849365944No Hit
GGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGA573100.20939035546780477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAACT570190.20832714497328148Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGTGCACCGCCGACCGACCTTGAGATCGGAAGAGCACACGTCTGAACT566330.20691683826920582Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGGTGTCCTAGGCGTAGAGGAACAACACCAGATCGGAAGAGCACACGT556380.20328146217968454No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGCAGATCGGAAGAGCACACGTCT540810.19759273798733817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA538140.19661721495628065Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGAG530840.19395005460919468No Hit
TCTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGT520980.19034756131847308No Hit
GACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACTC511710.186960633042105Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGAGGTAGTGACAATAAATAACAAGATCGGAAGAGCACACGTCTGAA494750.18076405229051895Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAA489770.17894453742360275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTCGCTGCCGTGATCGTGGCAGATCGGAAGAGCACACGTCTGAACT488880.17861936307991694Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTACGATAGGTGTCAAGTGGAAAGATCGGAAGAGCACACGTCTGAACTCC474920.17351887562165388Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACTCGACGGATCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA466620.17048635084346023Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTC460800.16835992985441361No Hit
CTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTGAACTCCAG449820.16434822840085142Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA448220.16376364531107912Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGTCGGCTCTTCCTATCATTGTGAGATCGGAAGAGCACACGTCTGAAC446710.16321194502010655Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAGC438150.1600844254898249No Hit
CGTTCGAATTGTAGTCTGGAGAAGCGAGATCGGAAGAGCACACGTCTGAA422630.1544139695190339Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGATGATTCATGATAACTCGATAGATCGGAAGAGCACACGTCTGAACT417590.15257253278625124Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACA414510.15144721033843964No Hit
TCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCA411030.15017574211818494Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACT408630.14929886748352653Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC406290.1484439147147346Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGTAGATCGGAAGAG397380.14518851763356527No Hit
AGGACGCACCTCTGGTGTACCAGTTATCGTGAGATCGGAAGAGCACACGT393390.1437307135534457No Hit
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACAC387730.14166275087337626No Hit
AGACGTCGCTGCCGTGATCGTGGTAAGATCGGAAGAGCACACGTCTGAAC387380.1415348733224886Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCACA371080.1355794330954336No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGA363560.13283189257350392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAAC329150.12025970249908906Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAT311210.11370506460501749TruSeq Adapter, Index 9 (97% over 36bp)
TGTGGAGACGTCGCTGCCGTGATCGTGAAGATCGGAAGAGCACACGTCTG304610.11129365935970688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGCGGATTACGGTGGCGAGATCGGAAGAGCACACGTCTGAACTCCA302790.1106286960950909Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACAC299510.10943030076105775No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGC298540.10907589726288332No Hit
GCCCATTAAAGCGGTACGTGAGCTGGAGATCGGAAGAGCACACGTCTGAA293970.10740618181272127Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACAC293190.1071211975564573No Hit
GTGGAGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTCT293140.10710292933490191Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTC290660.1061968255457549No Hit
TCTGATGATTCATGATAACTCGACGGATCGCACAGATCGGAAGAGCACAC288870.10554282321407217No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGACGTCGCTGCCGTGA283100.10343467044658093No Hit
CGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCAG282590.103248334586716Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACAC278170.10163342380122012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)