FastQCFastQC Report
Mon 25 Sept 2023
SRR23110909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23110909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28920085
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATG9813353.3932645771960903TruSeq Adapter, Index 7 (97% over 36bp)
AGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAAC7569422.617357452441789Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGA6019662.0814807425358532Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGAA5767051.9941331431079816Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC4998971.728546095213759Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTC4837971.6728754427934773RNA PCR Primer, Index 33 (100% over 34bp)
TCTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACT4761051.6462780105936756Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTC4085761.412775930637825No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCT3839901.3277623492462074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACT3324721.1496231771103025Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2724770.942172196243545No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTG2678100.9260346226506596Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTG2646820.9152186101804334Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGATGATTCATGATAACTCGACGGATCGCATAGATCGGAAGAGCACAC2494120.8624179354936198No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTG2381750.8235625863478616Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT1850600.6399013004284047No Hit
CTGATGATTCATGATAACTCGACAGATCGGAAGAGCACACGTCTGAACTC1816680.6281724275706658Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTACGATAGGTGTCAAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCA1725710.5967167800509576Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTG1565080.5411740663971077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCT1520900.5258974861242628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGA1388110.47998130019327395No Hit
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAG1197150.4139510654965226No Hit
GAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAAC1182030.4087228650953135Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCT1178330.4074434774309965Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTATCGTCACCGCAGTAGAGTTTAACCACCAAGATCGGAAGAGCACACGT1141760.39479828638124675No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGA1138590.393702162355332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACCCCAAGATGAGTGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCC1110290.3839165756255557Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGC970880.3357113231167889No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACA955840.33051078515156507No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG955510.33039667760312597TruSeq Adapter, Index 7 (100% over 36bp)
TCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC891800.308367005145386Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGA884390.30580477201225376Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGTCGGCTCTTCCTATCATTGTGAGATCGGAAGAGCACACGTCTGAAC860520.29755099267515983Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGTTTTTGGTCCCAAGGCTCGCAGATCGGAAGAGCACACGTCTGAACTC850100.2939479603880832Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCT843980.2918317840352129Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC838550.2899541961927152Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTCTGAAC836800.28934908040553825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG813370.2812474444663631Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCAC804010.2780109394560908No Hit
AGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAACTC795010.2748989154077521Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT743390.2570497285882804Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAG700320.24215696461473055No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTG693230.23970538122553928Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTTTTTGGTCCCAAGGCTCAGATCGGAAGAGCACACGTCTGAACTCCA649210.22448412582466476Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGGCTCCCAAA646110.22341220643023696No Hit
CGGGGAGGTAGTGACAATAAATAACAAGATCGGAAGAGCACACGTCTGAA635170.21962936830925636Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG627130.21684929349274043No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACAC626750.21671789692181057No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTGA622670.2153071126865637Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAA603860.20880298242553574Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACG589490.20383411736168824No Hit
TCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTC587260.20306302695859987No Hit
CGAGGCAAACTCTGAATACTAGATAGATCGGAAGAGCACACGTCTGAACT582850.2015381351749139Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGTC535530.18517580428964853No Hit
CGGATAACCGTAGTAATTCTAGAGCTAATACGTGAGATCGGAAGAGCACA534000.18464676020143095No Hit
CGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA524920.18150707371710698Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGTTTTTGGTCCCAAGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC522210.18057000869810721Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGC521320.1802622640977715No Hit
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGAG501510.17341235338692815No Hit
CTGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT499500.17271733468279918No Hit
GACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACACGTCTGAACTC490830.16971941818289954Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGTGCACCGCCGACCGACCTTGAGATCGGAAGAGCACACGTCTGAACT490400.1695707325894789Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACGCGGATTACGGTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA490350.16955344356698812Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAACT486780.1683190073611471Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATGAGATCGGAAGAGCACAC479910.1659434956709152No Hit
TCTGGTGTCCTAGGCGTAGAGGAACAACACCAGATCGGAAGAGCACACGT479850.1659227488439263No Hit
TCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACA468710.1620707546329826No Hit
TGTTTTTGGTCCCAAGGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA467320.16159011980773916Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTAGATCGGAAGAGCACAC465380.16091930573509725No Hit
TTACGATAGGTGTCAAGTGGAAAGATCGGAAGAGCACACGTCTGAACTCC461780.15967449611576176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCAAGGATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG459730.15896564619364018Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAGATCGGAAGAGC458820.15865098598430813No Hit
TGTGGAGACGTCGCTGCCGTGATCGTGGTTAGATCGGAAGAGCACACGTC453440.15679068716430122No Hit
TTCGGCGTTCGAATTGTAGTCTGAGATCGGAAGAGCACACGTCTGAACTC448630.1551274832006891Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTCGACGGATCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA441750.15274851370595904Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGAA439320.15190826721290757Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTC435800.1506911200295573No Hit
GCCCATTAAAGCGGTACGTGAGCTGGAGATCGGAAGAGCACACGTCTGAA418560.14472986507473956Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCACACGTCTG416890.14415241172354784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGACCCTCAGTAGGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA414430.14330179181700192Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGATGATTCATGATAACTCAGATCGGAAGAGCACACGTCTGAACTCCAG391170.1352589385542954Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACACGTCTGAACTCC382630.13230597351287177Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGATGATTCATGATAACTCGACGGATCGTAGATCGGAAGAGCACACGT372800.12890695169118624No Hit
TGGAGACGTCGCTGCCGTGATCGTGGAGATCGGAAGAGCACACGTCTGAA365530.12639312782102818Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGTCGGCTCTTCCTATCATAGATCGGAAGAGCACACGTCTGAACTCCA362830.12545952060652657Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGATGATTCATGATAACTCGATAGATCGGAAGAGCACACGTCTGAACT362660.12540073793005796Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGCGTTCGAATTGTAGTCTAGATCGGAAGAGCACACGTCTGAACTCCA347920.12030393409977874Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGAA347090.12001693632643196Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGAGACGTCGCTGCCGTGATCGTGGCAGATCGGAAGAGCACACGTCT338120.1169152856915877Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTAAGTCAGAATCCGGGCTAGAAGCGACGCATAGATCGGAAGAGCACA331840.1147437844667469No Hit
TGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAG326720.11297338856369199No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACAC321830.11128252216409461No Hit
CGTTCGAATTGTAGTCTGGAGAAGCGAGATCGGAAGAGCACACGTCTGAA320920.11096786195476259Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGAGGTAGTGACAATAAATAACAAGATCGGAAGAGCACACGTCTGAAC315980.10925970653267443Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAGACGTCGCTGCCGTGATCGTGGTCAGATCGGAAGAGCACACGTCTGA315160.10897616656382579Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGC314290.1086753375724864No Hit
CGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGAGCACACGTCTGAA312570.10808059519880388Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACCCCAAGATGAGTGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCA305160.10551836206567167Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGCGGGTAGAATCCTTTGCAGACGACTTAAATACGCGACGGGGTATTG300190.10379983323008907No Hit
CGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGA298810.10332265620934379No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAG297210.10276940748963911No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGATCGGAAGAGCA295910.1023198929048791No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)