FastQCFastQC Report
Mon 25 Sept 2023
SRR23190702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23190702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18610303
Total Bases930.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGAGAATTTGGACAAACTTGGTCATTCTGTAGGCACCATCAATAGAT6373023.4244579467620704No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG5235602.813280364107989No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA2863951.5389056266305818No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAG2135301.147375193192717No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTCTGTAGGCACCATCAATAGA1713160.9205438514354118No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGAT1506250.8093635014969933No Hit
AGCGTAGAATTTGGACAAACTTGGTCATTCTGTAGGCACCATCAATAGAT1271960.6834708709471308No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT786820.4227873130276278No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA762800.4098804839448342No Hit
TCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAA747200.40149803041895665No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTCTGTAGGCACCATCAATAGA735960.39545836518620897No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA680900.36587260293397694No Hit
TTGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATA637890.3427617486937209No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT589650.3168406231752379No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTACTGTAGGCACCATCAATAG575730.3093608954136856No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGA506680.2722577918263878No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA452660.24323085980921427No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTCTGTAGGCACATCAATAGATC450830.24224753353021708No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACATCAATAGA442620.23783599869384178No Hit
AGCTGAGAATTTGGACAAACTTGGTCATTCTGTAGGCACCATCAATAGAT416480.22379001567035206No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGA405260.21776109717289394No Hit
GCGGAGAATTTGGACAAACTTGGTCATTCTGTAGGCACCATCAATAGATC401740.21586967176192673No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA393480.21143127008732746No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGA368110.19779903637248678No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGAT360310.19360780960954801No Hit
AGCGTAGAATTTGGACAAACTTGGTCATTTCTGTAGGCACCATCAATAGA337270.18122757055594418No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT321320.17265704916249885No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATA319380.17161461583940896No Hit
TCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCA316120.16986289798720633No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG305500.16415638154843584No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGCACCATCAATAGA297540.15987918090318035No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAAT297460.159836193962022No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACATCAATAGAT286980.15420490467027861No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGACCATCAATAGA279060.1499491974956023No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACTTTGCTGTAGGCACCATCAATAG262700.14115836802872042No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTCTGTAGGCACCATCAATAGAT261240.14037385635258062No Hit
CTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAAT244090.13115853084176007No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA236070.12684908999063582No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGACCATCAATAGAT233340.12538216062360727No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG220760.11862246412645727No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATAG218900.11762301774452572No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTCTGTAGGACCATCAATAGATC214430.11522112240730309No Hit
TTCGATGTCGGCTCTTCCTATCATACCGAAGCTGTAGGCACCATCAATAG210500.11310938892289933No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATA210430.11307177534938577No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATAATAGA203520.10935877830683359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)