FastQCFastQC Report
Mon 25 Sept 2023
SRR23190703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23190703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19012888
Total Bases950.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG5857823.080973285068528No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA2151041.131358897185951No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAG1777380.9348290485906192No Hit
TCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAA1666740.8766369422677922No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1160750.610506936137214No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1124160.5912620954796557No Hit
TTGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATA1003980.5280523400758474No Hit
TCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCA705000.3708011113303776No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACATCAATAGA699620.36797145178575713No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGA682210.3588145051924779No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTCTGTAGGCACCATCAATAGA606860.31918349279709635No Hit
CTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCA518010.2724520335890055No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT515660.27121602988457094No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA503560.26485192570429067No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGAT499380.26265341698746664No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGCACCATCAATAGA494030.25983953621354106No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGACCATCAATAGA479290.2520869002121087No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTACTGTAGGCACCATCAATAG469670.2470271744092744No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT421270.22157075768815343No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA417990.21984561209217662No Hit
CTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAAT360930.18983439023045842No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAAT328350.1726986452557865No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA317750.16712347961025173No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCA310080.16308937390258652No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA309530.1628000964398465No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA306390.1611485851071126No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATAATAGA295430.15538407421323894No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTCTGTAGGCACCATCA281450.14803116706941102No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG272170.1431502673344523No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGA264000.13885318211520523No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG250180.1315844284150835No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG245100.12891255657741213No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACATCAATAGAT234410.12329005461979264No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTCTGTAGGCACCATC232070.12205931050558967No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATA229950.12094427737648274No Hit
TCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACATCAAT222330.11693646961997567No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCCTGTAGGCACCATCAAT221460.11647888526982329No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCTCAATAGA221020.11624746329963129No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACTTTGCTGTAGGCACCATCAATAG219770.1155900145206767No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATA219100.11523762197515705No Hit
TGTATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG215490.11333890990153626No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGAT211640.11131396766235618No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTTGTAGGCACCATCAATAG210440.11068281683455979No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGACCATCAATAGAT210030.1104671736350627No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATAG204420.1075165435151146No Hit
TTTTTATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA202370.10643832751762909No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT201420.10593866644562362No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACTATCAATAG197650.10395580092829665No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCCCATCAATAGA196340.10326679460795225No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)