FastQCFastQC Report
Mon 25 Sept 2023
SRR23229748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23229748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50552725
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8944301.7693012592298436Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATAG5924761.1719961683568987No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAG4393980.8691875660510882No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGA3744200.740652457409566No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATAGA3173420.6277445973486098No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA3153220.623748769230541No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2926430.5788866970079259Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT2723310.5387068649612855No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA2608380.5159721854756594No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT2479090.49039690738728725No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA1995580.39475221167602736No Hit
CGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1928680.38151850370083906No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAGAT1913630.3785414139396046No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATA1817490.3595236458568752No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT1693060.33490974027611764No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC1601400.3167781756571975No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATAG1597350.315977031900852No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG1563020.30918610223286674No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1508190.2983400004648612No Hit
TCGACTGCATAATTTGTGGTAGTCTGTAGGCACCATCAATAGATCGGAAG1411180.2791501348344723No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAG1342820.2656276194804533No Hit
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCTGTAGGCACCATCAATAGA1276670.25254227146014385No Hit
CTACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGA1272670.25175101836745695Illumina Single End PCR Primer 1 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCA1244670.24621224671864872No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA1205150.2383946661629022No Hit
CACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCA1153470.22817167620538753No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATA1133960.2243123392458072No Hit
ACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1119700.22149152197037847No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGCTGTAGGCACCATCAATAG1085540.21473422055883237No Hit
TGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCTGTAGGCACCATCAATAGA1051610.2080224162001158No Hit
TACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAG1034990.20473475960000176Illumina Single End PCR Primer 1 (100% over 21bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCTGTAGGCACCATCAATAGAT1014220.20062617791622508No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA1002370.19828209062914018No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA968490.1915801769340822No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA966070.1911014688130066No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT932170.18439559885248522No Hit
ACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGC911000.1802078918594398No Hit
GGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATAGA910150.18003975057724386No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA908580.17972918373836425No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC904930.17900716529128746No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT862550.17062383877426984No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCCTGTAGGCACCATCAATAGA851690.1684755866276249No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC850560.16825205762894088No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC798550.15796378929127955No Hit
GGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATAGAT788450.15596587523224514No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGAT767760.1518731186103222No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAATA750330.14842523325893905No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG744680.14730758826551882No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG723970.14321087537813243No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT710730.14059182764133882No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGCTGTAGGCACCATCAATAGAT699270.13832488753079086No Hit
CACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAG694480.13737736195229833Illumina Single End PCR Primer 1 (95% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAAT683790.1352627380620926No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA669240.13238455493744403No Hit
TTAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA669030.13234301415007796No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT660680.13069127331909408No Hit
ACCCCTCCCACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC648860.1283531204302043No Hit
GACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC628520.12432959845389145No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA627190.12406650680057306No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG626400.12391023431476741No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA620060.12265609816285868No Hit
CGACGCTCTTCCGATCTTGTAGGCACCATCAATAGATCGGAAGAGCACAC619710.12258686351724858No Hit
TACACGACGCTCTTCCGATCTTGTAGGCACCATCAATAGATCGGAAGAGC618230.12229409987295442Illumina Single End PCR Primer 1 (100% over 21bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT607620.12019530104460244No Hit
CTACACGACGCTCTTCCGATCTTGTAGGCACCATCAATAGATCGGAAGAG606110.11989660300211315Illumina Single End PCR Primer 1 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGATC605150.11970670225986829No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAG603250.11933085704084201No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAGA595300.1177582415191268No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGA591550.11701644174473284No Hit
TTAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT588840.11648036777443747No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA572370.1132223831652992No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAG551810.10915534226888857No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA539690.10675784539804728No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAG533440.10552151244072401No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT531830.10520303307091754No Hit
TCGGGGAGCGGTCGGGCGGCGGCGGTCGGCTGTAGGCACCATCAATAGAT530110.1048627942410622No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGCTGTAGGCACCATCAATAGATCG516500.10217055559319503No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT513780.10163250349016795No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGAT510920.10106675752889682No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)