FastQCFastQC Report
Mon 25 Sept 2023
SRR23229749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23229749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53209122
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC8256011.5516155293823493No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6976071.3110665498295573Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGA5803271.0906532154392625No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAG5768021.084028411519363No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT4975310.935048317467069No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC4405230.82790879353356No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAGAT3557160.6685244684172763No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATAG3162460.5943454582843897No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3078840.5786301078224895Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2955350.5554216812673586No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGAT2897310.5445137771677571No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA2682030.5040545491429083No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCTGTAGGCACCATCAAT2257990.42436144689626715No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2163770.4066539568159009No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATAGA2146620.4034308252633825No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATA1911730.3592861389443712No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1846950.3471115347477449No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA1835500.34495964808440177No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAG1789500.33631451389105804No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1737620.3265643060225651No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA1689810.3175790045924832No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT1646190.30938116212479505No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG1575250.29604886169706013No Hit
TGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCTGTAGGCACCATCAATAGA1467450.27578917765265887No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG1441500.27091219434141384No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA1328270.24963200858679835No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1261380.23706085584347739No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1214890.2283236321772045No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA1182960.22232278142082482No Hit
TCGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG1180760.22190931848114315No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA1178160.22142068046151936No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGAT1141730.2145741100557908No Hit
CTACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGA1124050.2112513715223491Illumina Single End PCR Primer 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1113060.2091859362009394No Hit
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCTGTAGGCACCATCAATAGA1112220.20902806853306094No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1110140.20863715811736191No Hit
TTAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA1040610.19556984984642295No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGCTGTAGGCACCATCAATAGAT1031890.19393103310368473No Hit
TCCCTACACGACGCTCTTCCGATCTTAGGCACCATCAATAGATCGGAAGA1000380.18800911618124425Illumina Single End PCR Primer 1 (100% over 25bp)
TAACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAGA955760.1796233360137008No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA953330.17916664740305244No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT903720.16984305811323103No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA873000.16406961197367625No Hit
TTAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA862100.16202109104525347No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGAT814490.1530733771551427No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT814170.153013237091189No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA785450.1476156663513448No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA781300.14683572489694532No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT779350.14646924638222747TruSeq Adapter, Index 1 (100% over 35bp)
TCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGATC779330.14646548762823036No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATAG766590.14407116133207384No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA761410.1430976440468234No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG726270.13649351327390818No Hit
CCCTACACGACGCTCTTCCGATCTTAGGCACCATCAATAGATCGGAAGAG721670.13562899985457383Illumina Single End PCR Primer 1 (100% over 24bp)
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG713900.13416872392669812No Hit
CTACACGACGCTCTTCCGATCTTGTAGGCACCATCAATAGATCGGAAGAG705730.13263327291888036Illumina Single End PCR Primer 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT697610.1311072187960553No Hit
GGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATAGAT697480.1310827868950741No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT649840.12212943487396766No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATA648710.12191706527313118No Hit
CCTACACGACGCTCTTCCGATCTTAGGCACCATCAATAGATCGGAAGAGC646980.12159193305238152Illumina Single End PCR Primer 1 (100% over 23bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAG623180.11711901579582538No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA622290.11695175124295416No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGATC621580.11681831547605691No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT613220.11524715630526661No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA607870.11424168961104075No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA596630.11212926986466719No Hit
TACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAG584190.10979132487846727Illumina Single End PCR Primer 1 (100% over 21bp)
GGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATAGA574780.1080228311228289No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG561710.10556648538572012No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGA561390.10550634532176645No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA556780.10463995252543352No Hit
TTAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT554700.1042490421097345No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAA535650.10066882892749104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)