FastQCFastQC Report
Sat 1 Jul 2023
SRR2340141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2340141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39351578
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTA18101384.59991210517657No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG10163822.5828239975535414Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3137520.797304748490645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA1844230.4686546496305688No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC1475550.37496590352742654No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCTGTAGGCACCAT1288570.3274506552189597No Hit
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1226960.31179435803057254No Hit
ACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1171280.29764498897604563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGTGCTGAAGCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1135030.2884331601645047No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCACTGTAGGCACC1024410.2603224704229142No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA1020810.2594076405271473No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA897140.22798069241340208No Hit
ATGTACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC884860.22486010599117526No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA845860.21494944878703465Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGCACC820080.20839825025568223No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC818810.20807551859800896No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC810900.20606543402147687No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAG753680.19152472106709417No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA747290.18990089800210808No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC688290.1749078524881518No Hit
ACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT684660.17398539900992027No Hit
TACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG671590.1706640582494557No Hit
ACGCGATGTACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAG648660.16483710005225205No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATAGATC646240.16422213106676434No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA644580.16380029283704964No Hit
AAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA638430.1622374584317813Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGCA598920.1521972003257404No Hit
TAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATC578230.1469394696192361No Hit
TACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGC557410.141648703388718No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT555050.14104898156815973No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCACTGTAGGCACC533770.13564132040651586No Hit
ATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTGTAGGCAC531470.13505684575088703No Hit
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG510090.12962377264769406No Hit
TTTCCCTGGTTCGAATCCAGGTACCCCAGCCACTGTAGGCACCATCAATA508110.12912061620502233No Hit
TCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGCACCATCAAT484960.12323775173641066No Hit
TCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCA476760.12115397252938626No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT467450.11878812077116703No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA442990.11257235986826247No Hit
TCGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC431150.10956358598885159No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAG426920.10848866086132557No Hit
TGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAG410700.10436684394206505No Hit
TATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGT404270.10273285610045929No Hit
AACGCGATGTACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAA403560.10255243131546085No Hit
TTAACGGGGCTAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC400700.1018256497871572No Hit
AAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATC397970.10113190378286736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)