FastQCFastQC Report
Sat 1 Jul 2023
SRR2340143.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2340143.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39177034
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG7667531.9571491808185377Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC2706660.6908792533911576No Hit
ACGGTGCTGAAGCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2492080.6361073684138518No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA2279480.581840881573628No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC2035680.5196105453005962No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTA1972240.50341738478722No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA1542960.39384298464248213No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC1413610.36082619220229895No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA1411140.3601957207888683No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC1401100.3576329948816442No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1125060.2871733475280441Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT1071150.2734127346138556No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAG1063120.27136306439124513No Hit
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC962480.2456745449387516No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCACTGTAGGCACC954960.24375505302417738No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCTGTAGGCACCAT866690.2212239956705247No Hit
ACGGTGCTGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC853930.21796698545377374No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA817660.20870901048813448No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCACTGTAGGCACC802690.2048878942698929No Hit
AAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA788040.20114845855865454Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT712380.18183612368409513No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATAGATC675370.17238926254601103No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAG671580.17142185904119236No Hit
ATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTGTAGGCAC637020.16260036428485117No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA617650.1576561410953162No Hit
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG616210.15728857881380198No Hit
ACGTATCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGCACCATCA597400.15248729651152254No Hit
TAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCA565430.14432690335873818No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC559090.1427086083137381No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATA544660.1390253279510644No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA544060.13887217700043347No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA519910.1327078512375388No Hit
TCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATC498190.1271637868246994No Hit
TTAACGGGGCTAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC497860.12707955380185237No Hit
TTTCCCTGGTTCGAATCCAGGTACCCCAGCCACTGTAGGCACCATCAATA494230.1261529905505353No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA477830.1219668645666234No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT477410.12185965890118176No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT449400.11471006202256148No Hit
TAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATC446210.11389580946837374No Hit
TCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCA444870.11355377234529801No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAA444170.1133750962362286No Hit
TCGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC441630.11272675721189103No Hit
AAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT438340.11188697949926481Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT426740.10892606112040029No Hit
AAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT423450.10808628340777406No Hit
TCGGACGCGGGTTCAACTCCCGCCAGCTCCACCACTGTAGGCACCATCAA421910.10769319596782136No Hit
AAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATC416540.10632249495967458No Hit
TTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCACTGTAGGCACCATC406250.10369595615635424No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)