FastQCFastQC Report
Sat 1 Jul 2023
SRR2340144.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2340144.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18366041
Total Bases918.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC1221680.6651841842234807No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG964140.5249579917631677No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTA886120.4824774157914599No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC829430.4516106655756676No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT666060.36265845208556374No Hit
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC514050.27989156726809006No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCTGTAGGCACCAT495490.269785959859286No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCACTGTAGGCACC450960.24554012484236531No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA408420.22237781130947054No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCACTGTAGGCACC399560.21755369053134532No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT351030.19112992288321692No Hit
TGATGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT348820.18992661510447462No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATA336970.18347448968452157No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATAGATC324890.17689713313827404No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAG307600.16748301933987841No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATAGATCG289590.15767687766786537No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA278900.1518563527109626No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCTGTAGGCACCATCAA272580.1484152191536543No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC263460.14344953275450054No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA244280.13300634578785922No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGCTGTAGGCACCATCAA239900.1306215095566867No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCTGTAGGCACCATC232000.12632009261005134No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCCTGTAGGCACCATCAAT231920.1262765339574272No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATA230650.12558504034701873No Hit
ATGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT230500.12550336787334843Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATCAATAGATCGGA230460.12548158854703637No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT230030.12524746078918153No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC218220.11881711469554053No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA208660.11361185570695394No Hit
TAACCGTGAGGCTTAACCTCTGTAGGCACCATCAATAGATCGGAAGAGCA208630.11359552121221989No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTCTGTAGGCACCATCAATA208470.11350840390697158No Hit
TCGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC199650.1087060624551584No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT195490.1064410125187023No Hit
TCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCA186700.10165500556162321No Hit
TATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCACTGTAGGCAC184990.1007239393617819No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)