FastQCFastQC Report
Mon 25 Sept 2023
SRR23433735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23433735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96803945
Total Bases14.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA35847183.7030701589692447No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA34024023.514734859204344RNA PCR Primer, Index 1 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC27312182.8213912149964546No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC26862612.774949925852712No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA22767422.351910348281777No Hit
TGGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA20820282.150767719228798RNA PCR Primer, Index 9 (100% over 35bp)
TGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG13068161.3499615124156354RNA PCR Primer, Index 6 (100% over 34bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC12735451.3155920453448462No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG10805681.11624376465236No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA10628091.097898437920066No Hit
GTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC10433101.0777556637800247RNA PCR Primer, Index 1 (100% over 33bp)
GAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGT10193661.0530211346242138No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT9950031.0278537718684915No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG9437670.9749261768205831Illumina Small RNA Adapter 2 (100% over 21bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGG9283010.9589495552066603No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG8515490.8796635302414587No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG8120870.8388986626526429No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC8043000.8308545689950961No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA7154720.739093845813825No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC6821330.7046541336719283No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG5972900.6170099782607No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG5916040.611136250697221Illumina Small RNA Adapter 2 (100% over 21bp)
ATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC5882630.6076849450711952RNA PCR Primer, Index 1 (100% over 33bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA5779670.5970490148929365RNA PCR Primer, Index 1 (100% over 22bp)
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGG5236420.5409304341884No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG5236010.5408880805425853Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGG4620150.4772687724658328Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA4616220.47686279727546227RNA PCR Primer, Index 1 (100% over 23bp)
TGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4612590.47648781255763906RNA PCR Primer, Index 1 (100% over 33bp)
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT4372170.45165204785817353No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCAA4316370.44588781996436194No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG4300910.4442907776124207No Hit
TGGGAAATGTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT4125540.4261747803769774RNA PCR Primer, Index 9 (100% over 36bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC4067170.42014506743501No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCTGGAATTCTCGGGTG4029580.416261961224824No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCAA3817030.3943052114250096No Hit
ATGGATTTTTGGAAGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3734160.38574460989167336RNA PCR Primer, Index 1 (100% over 31bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC3681410.3802954518020934RNA PCR Primer, Index 1 (100% over 24bp)
GAGGTGGAGCACGAGCGTACGCGTTAGGACCCGATGGAATTCTCGGGTGC3588480.3706956364226685No Hit
GTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3491040.3606299309392815RNA PCR Primer, Index 1 (100% over 32bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGA3403810.35161893453825666RNA PCR Primer, Index 1 (100% over 22bp)
AAGGTGAACAGCCTCTGGCATGTTGGAACTGGAATTCTCGGGTGCCAAGG3387490.3499330528316795Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG3368420.34796309179341817No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAAGGA3284510.3392950566219176RNA PCR Primer, Index 1 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAG3225040.33315171194727655No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAGG3169780.32744326690405023Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG2983850.30823640503493943RNA PCR Primer, Index 9 (100% over 39bp)
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCTGGAATTCTCGGGTGC2803820.2896390224592603No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAG2784070.28759881634989154No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATGGAATTCTCGGGTGCCAA2688080.2776828981504834No Hit
GAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGC2642130.2729361907719773No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCCA2595220.26809031388131965No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG2510540.25934273649694756No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGTGGAATTCTCGGGTGCCA2491300.2573552141909093No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGGG2490790.2573025303875787No Hit
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG2397150.24762937088979176Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACTGGAATTCTCGGG2376720.24551891970931555No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCTGGAATTCTCGGGT2325180.24019475652567673No Hit
GTGGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2233660.23074059636722452RNA PCR Primer, Index 6 (100% over 34bp)
CGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCCA2220350.22936565240187268No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTGGAATTCTCGGGTGCC2168890.22404975334424643No Hit
CATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1980460.20458463753724085RNA PCR Primer, Index 1 (100% over 32bp)
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGATGGAATTCTCGGGTG1975290.20405056839367447No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGAAGTTGGAATTCTCGGGTGCCA1949950.20143290647917292No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGGGTGGAATTCTCGGGT1915980.19792375197105863No Hit
TTTCGCCGAATCCCGGGGCCGAGGAATGGAATTCTCGGGTGCCAAGGAAC1831400.18918650474420232RNA PCR Primer, Index 1 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCCA1804560.18641389046696394No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCAA1797340.18566805309432377No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGAA1791030.18501622015507735RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1743920.18014968294938805RNA PCR Primer, Index 1 (100% over 23bp)
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACTGGAATTCTCGGGTGCC1704680.17609612914019154No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1669330.1724444184583593No Hit
AGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGTG1640350.169450738810283No Hit
ACGAGCGTACGCGTTAGGACCCGAAAGATGGTGGAATTCTCGGGTGCCAA1585060.16373919471980197No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG1582540.16347887475040404No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA1574330.16263076881835756RNA PCR Primer, Index 1 (100% over 22bp)
TGGCATGTTGGAACAATGTAGGTAAGGGAAGTGGAATTCTCGGGTGCCAA1558910.16103785852942254No Hit
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA1470450.15189980119095353RNA PCR Primer, Index 9 (100% over 38bp)
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGTGGAATTCTCGGGT1458810.15069737085611543No Hit
TGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1455530.15035854168959745RNA PCR Primer, Index 1 (100% over 32bp)
ACCAGGAGTGGAGCCTGCGGCTTAATTTGTGGAATTCTCGGGTGCCAAGG1437300.1484753539744687Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGAAATGTGGCGTCCCGGAGTCGGGTTGCTTGGAATTCTCGGGTGCCA1397710.1443856446139669No Hit
TGGGAAATGTGGCGTCCCGGAGTCGGGTTGCTGGAATTCTCGGGTGCCAA1389350.14352204344564676No Hit
GTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1348640.13931663632096813RNA PCR Primer, Index 1 (100% over 31bp)
AATTCTCCAGGCATACGCGTATAACAACTCTGGAATTCTCGGGTGCCAAG1339940.13841791261709427No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA1339450.13836729484526689No Hit
GGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT1336880.13810180979711106RNA PCR Primer, Index 9 (100% over 36bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1335510.13796028663914472RNA PCR Primer, Index 1 (100% over 25bp)
TGGGAAATGTGGCGTCCCGGAGTCGGGTTTGGAATTCTCGGGTGCCAAGG1318770.13623101827100126Illumina Small RNA Adapter 2 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGGT1243020.12840592395278932No Hit
AATTCTCCAGGCATACGCGTATAACAACTGGAATTCTCGGGTGCCAAGGA1219280.12595354455854046RNA PCR Primer, Index 1 (100% over 22bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGGT1202580.12422840825340331No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1201160.12408172001667907No Hit
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG1196100.12355901404638003No Hit
TGCATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1176420.12152603904727231RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1162020.12003849636499835No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTTGGAATTCTCGGGTGCC1157910.11961392689109933No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCG1157060.11952612055221509No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCTGGAATTCTCGGGTGCC1150730.11887222158146551No Hit
GGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGTGC1141560.11792494613726745No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC1141540.11792288010576428RNA PCR Primer, Index 9 (100% over 50bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG1141150.1178825924914527No Hit
TGCATGATGACGAACAAATACTGACTACCTGAAGATGGAATTCTCGGGTG1141060.11787329534968848No Hit
CCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGGAATTCTCGGG1062790.10978788106207862No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATGGAATTCTCGGGTGCCAAG1050910.1085606583492026No Hit
AGCCTCTGGCATGTTGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1028580.1062539341759264RNA PCR Primer, Index 1 (100% over 30bp)
ATGGATTTTTGGAACTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1014370.10478601879293245RNA PCR Primer, Index 1 (100% over 31bp)
AGGTGTCCTAAGGCGAGCTCAGGGAGGACTGGAATTCTCGGGTGCCAAGG1013340.1046796181705198Illumina Small RNA Adapter 2 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCAA1001060.10341107482758063No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGTG989800.10224789909130252No Hit
CTAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC985060.10175824962505402RNA PCR Primer, Index 1 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGT983050.10155061345898661No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGTGGAATTCTCGGG974950.10071387070020753No Hit
TGCGGCCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA969100.10010955648553373RNA PCR Primer, Index 9 (100% over 38bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA969090.10010852346978213RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)