FastQCFastQC Report
Mon 25 Sept 2023
SRR23433736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23433736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77216259
Total Bases11.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC23364843.0258963983220166No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA21787662.8216414887439702No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC19597992.538065202045077No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA17999152.3310051837657664No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA16994382.2008810346535954RNA PCR Primer, Index 1 (100% over 22bp)
TGGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC14379621.8622528708623398RNA PCR Primer, Index 5 (100% over 35bp)
GAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGT11202741.4508265674979153No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA10896811.4112066734546151No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC10823101.4016607564476802No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT10613291.3744890179152554No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGG9894361.2813829791987204No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG8524331.1039553211196103No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG8145471.0548905250641578No Hit
TGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA7467450.967082593317555RNA PCR Primer, Index 1 (100% over 34bp)
GTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC7111620.921000329736254RNA PCR Primer, Index 1 (100% over 33bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG6308180.8169497048542588Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC6235440.8075294090587838No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG6193920.8021523031826756No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC5875100.760863071597395No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG5516920.7144764679677112No Hit
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGG5391250.6982013982314269No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA5091800.6594207056832422No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT4894130.6338211748901226No Hit
ATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4545790.5887089142715397RNA PCR Primer, Index 1 (100% over 33bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG4322210.559753872561995No Hit
ATGGATTTTTGGAAGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC4216390.5460495049365186RNA PCR Primer, Index 1 (100% over 31bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA4208680.5450510105650158RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG4086090.5291748205517183Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3750400.48570081593825987RNA PCR Primer, Index 1 (100% over 33bp)
GTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3448320.4465795215486935RNA PCR Primer, Index 1 (100% over 32bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC3367340.43609209298782525No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCTGGAATTCTCGGGTG3314840.42929300680054955No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG3289690.4260359207508357Illumina Small RNA Adapter 2 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCAA3277660.4244779587159228No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAG3275230.42416325815525463No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGG3138370.40643901176305364Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGAAATGTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT3020750.39120646857548486RNA PCR Primer, Index 8 (100% over 36bp)
GAGGTGGAGCACGAGCGTACGCGTTAGGACCCGATGGAATTCTCGGGTGC3012410.3901263851697348No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG3009660.3897702425599251No Hit
GAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGC2903580.37603220326952125No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG2860520.37045565753191956No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA2798930.36247935813621845RNA PCR Primer, Index 1 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAGG2591630.3356326806767471Illumina Small RNA Adapter 2 (100% over 21bp)
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCTGGAATTCTCGGGTGC2441180.31614844226006855No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAG2432420.3150139661648203No Hit
AGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACTGGAATTCTCGGG2409700.3120715806757745No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCTGGAATTCTCGGGT2384970.3088688873155588No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAAGGA2343950.303556534641234RNA PCR Primer, Index 1 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2329670.30170718319829504RNA PCR Primer, Index 1 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG2253160.291798648261372No Hit
CGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGA2236570.2896501370261929RNA PCR Primer, Index 8 (100% over 39bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT2139950.2771372283135343No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGA2026750.26247710342973235RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCCA1987440.2573862067055075No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCAA1973660.25560160846435204No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGTGGAATTCTCGGGTGCCA1963860.2543324457093939No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGGG1912710.24770819316693393No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATGGAATTCTCGGGTGCCAA1842020.2385533854987717No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGGGTGGAATTCTCGGGT1812750.23476273306636108No Hit
TGCATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1796330.23263623791978838RNA PCR Primer, Index 1 (100% over 30bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCG1773460.22967442647020753No Hit
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG1746380.22616739306160893Illumina Small RNA Adapter 2 (100% over 21bp)
TGCATGATGACGAACAAATACTGACTACCTGAAGATGGAATTCTCGGGTG1621510.20999592844817824No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGAAGTTGGAATTCTCGGGTGCCA1619430.20972655512875854No Hit
AGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGTG1602720.20756250312515143No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCCA1598720.20704447751088276No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGTGGAATTCTCGGGT1540620.19952015546363105No Hit
GTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1530160.1981655184823186RNA PCR Primer, Index 1 (100% over 31bp)
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGATGGAATTCTCGGGTG1523600.19731595647491806No Hit
CATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1505260.19494080903349642RNA PCR Primer, Index 1 (100% over 32bp)
GTGGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1435220.18587018052765286RNA PCR Primer, Index 1 (100% over 34bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC1433640.18566556041001675No Hit
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTG1432920.1855723157994484RNA PCR Primer, Index 8 (100% over 38bp)
TGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1354200.17537757171064194RNA PCR Primer, Index 1 (100% over 32bp)
CGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCCA1350460.17489321776130076No Hit
AAGGTGAACAGCCTCTGGCATGTTGGAACTGGAATTCTCGGGTGCCAAGG1312760.170010826346819Illumina Small RNA Adapter 2 (100% over 21bp)
TGGCATGTTGGAACAATGTAGGTAAGGGAAGTGGAATTCTCGGGTGCCAA1302050.16862381276461477No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGGT1286430.1666009227408958No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGTGGAATTCTCGGG1236640.16015279890728712No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGAA1223930.1585067725179486RNA PCR Primer, Index 1 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG1216880.15759375237280016No Hit
ACGAGCGTACGCGTTAGGACCCGAAAGATGGTGGAATTCTCGGGTGCCAA1183620.15328636939015655No Hit
TGGGAAATGTGGCGTCCCGGAGTCGGGTTGCTTGGAATTCTCGGGTGCCA1148140.14869148219159387No Hit
TTTCGCCGAATCCCGGGGCCGAGGAATGGAATTCTCGGGTGCCAAGGAAC1125290.14573226087008437RNA PCR Primer, Index 1 (100% over 24bp)
ATGGATTTTTGGAACTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1122540.1453761182602747RNA PCR Primer, Index 1 (100% over 31bp)
ATAATTTGTGGTAGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1090270.1411969466171626RNA PCR Primer, Index 1 (100% over 32bp)
TGGGAAATGTGGCGTCCCGGAGTCGGGTTGCTGGAATTCTCGGGTGCCAA1086200.14066985555464426No Hit
CCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGGAATTCTCGGG1082230.14015571513248265No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTTGGAATTCTCGGGTGCC1074900.1392064331943354No Hit
AATGGATTTTTGGAAGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1073360.13900699333284197RNA PCR Primer, Index 1 (100% over 30bp)
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG1034960.13403394743586322No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGTGGAATTCTCGGGTGCCAAGG970130.12563804729260453Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA968700.1254528531355035RNA PCR Primer, Index 1 (100% over 22bp)
AATTCTCCAGGCATACGCGTATAACAACTCGGATTGGAATTCTCGGGTGC964980.12497108931423367No Hit
GGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGTGC961930.12457609478335385No Hit
GGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT957790.12403993827258583RNA PCR Primer, Index 8 (100% over 36bp)
TGCGGCCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTG933140.12084760542465545RNA PCR Primer, Index 8 (100% over 38bp)
AATTCTCCAGGCATACGCGTATAACAACTCTGGAATTCTCGGGTGCCAAG895530.11597686958649472No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGG892650.11560389114422132No Hit
TGCATGATGACGAACAAATACTGACTACCTGAAGATTGGAATTCTCGGGT890600.11533840301690866No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCAA875900.11343465888447148No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGGT858770.1112162141913661No Hit
TGGGAAATGTGGCGTCCCGGAGTCGGGTTTGGAATTCTCGGGTGCCAAGG848490.10988488836269573Illumina Small RNA Adapter 2 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGTGGAATTCTCGGGTGC833470.10793970218111706No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGGT828040.1072364824097474No Hit
TCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGGAATTCTCGG826910.10709014017371651No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCAA810080.1049105474016813No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC807580.10458678139276341RNA PCR Primer, Index 8 (100% over 50bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA801660.10382010348364584RNA PCR Primer, Index 1 (100% over 23bp)
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGTGGAATTCTCGGGTGCC780500.10107974798416483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)