FastQCFastQC Report
Mon 25 Sept 2023
SRR23433737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23433737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106000544
Total Bases15.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA37190663.508534824123167No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC33887453.1969128384850554No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC29902642.8209892960549334No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA28810132.7179228438676692No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA26412472.4917296650854923RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA18285481.7250364300017178No Hit
TGGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA16842141.5888729778594342RNA PCR Primer, Index 7 (100% over 35bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC13134281.2390766598329912No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT11493701.0843057560157427No Hit
GAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGT11273001.063485108151898No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC10952531.0332522444413117No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG10892261.0275664245647644No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG9887910.9328169108264198Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC9865230.9306772991655591No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG9338590.8809945352733285No Hit
TGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC8710600.8217504996955487RNA PCR Primer, Index 2 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA8467130.7987817496483791No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGG8159210.7697328421257914No Hit
GTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC8011320.7557810269351071RNA PCR Primer, Index 1 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG7924680.7476074839766861No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG7502840.707811461797781No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG7500980.7076359910001971No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG7386880.6968718952989524Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA6329850.597152595745169RNA PCR Primer, Index 1 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAG6243360.5889932036575208No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG5864830.5532830095664415Illumina Small RNA Adapter 2 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCAA5457560.5148615086352764No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG5355490.5052323127700176No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT5202970.4908437073681433No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGG5064330.47776452920845386Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGATTTTTGGAAGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC4993120.47104663915687073RNA PCR Primer, Index 1 (100% over 31bp)
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGG4941270.4661551548263752No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCTGGAATTCTCGGGTG4682870.44177792144161077No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAGG4638570.43759869760668396Illumina Small RNA Adapter 2 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAG4418890.41687427566409474No Hit
TGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4383150.41350259485460755RNA PCR Primer, Index 1 (100% over 33bp)
GTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4271040.40292623403894984RNA PCR Primer, Index 1 (100% over 32bp)
GAGGTGGAGCACGAGCGTACGCGTTAGGACCCGATGGAATTCTCGGGTGC4240400.40003568283574087No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC4151910.3916876124711209No Hit
ATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4090960.3859376419804034RNA PCR Primer, Index 1 (100% over 33bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAAGGA3898190.3677518862544706RNA PCR Primer, Index 1 (100% over 22bp)
TGGGAAATGTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG3774970.35612741761023414RNA PCR Primer, Index 7 (100% over 36bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA3719980.3509397083848928RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG3468280.327194547227984No Hit
GAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGC3423450.3229653236496598No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCAA3261540.3076908737374027No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATGGAATTCTCGGGTGCCAA3194170.3013352459776055No Hit
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG2977540.28089855840739836Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC2945330.27785989475676653RNA PCR Primer, Index 7 (100% over 39bp)
TGCATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2930220.2764344303742441RNA PCR Primer, Index 1 (100% over 30bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGA2840770.2679957944366776RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCCA2803910.2645184537920862No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2796490.2638184573845206RNA PCR Primer, Index 1 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG2644880.24951570059866865No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCTGGAATTCTCGGGT2627010.24782986019392506No Hit
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG2494310.235311056516842No Hit
CATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2462330.232294090868062RNA PCR Primer, Index 1 (100% over 32bp)
TGGCATGTTGGAACAATGTAGGTAAGGGAAGTTGGAATTCTCGGGTGCCA2311680.2180818996551565No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCCA2287330.21578474163302408No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGTGGAATTCTCGGGTGCCA2158160.2035989551147964No Hit
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCTGGAATTCTCGGGTGC2132860.20121217491110235No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT2122670.2002508590899307No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCCA1981900.18697073856526622No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGAAGTGGAATTCTCGGGTGCCAA1981710.1869528141289539No Hit
GTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1932980.18235566791053448RNA PCR Primer, Index 1 (100% over 31bp)
TGGGAAATGTGGCGTCCCGGAGTCGGGTTGCTTGGAATTCTCGGGTGCCA1931390.18220566868034188No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGAA1924650.1815698228869467RNA PCR Primer, Index 1 (100% over 23bp)
TGGGAAATGTGGCGTCCCGGAGTCGGGTTGCTGGAATTCTCGGGTGCCAA1916210.18077360055812544No Hit
AGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGTG1866890.1761207942479993No Hit
TGCATGATGACGAACAAATACTGACTACCTGAAGATGGAATTCTCGGGTG1845860.1741368421656402No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGTGGAATTCTCGGGT1831100.17274439648158785No Hit
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGATGGAATTCTCGGGTG1791590.16901705712000875No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGTGGAATTCTCGGGTGCCAAGG1777110.16765102639473248Illumina Small RNA Adapter 2 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCAA1765080.16651612655874673No Hit
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT1734790.16365859405400787RNA PCR Primer, Index 7 (100% over 38bp)
TTTCGCCGAATCCCGGGGCCGAGGAATGGAATTCTCGGGTGCCAAGGAAC1730830.16328501106560359RNA PCR Primer, Index 1 (100% over 24bp)
AATGGATTTTTGGAAGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1664140.15699353391997686RNA PCR Primer, Index 1 (100% over 30bp)
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA1663220.1569067419125698RNA PCR Primer, Index 1 (100% over 22bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGGGTGGAATTCTCGGGT1645390.15522467507336565No Hit
TGGGAAATGTGGCGTCCCGGAGTCGGGTTTGGAATTCTCGGGTGCCAAGG1638820.15460486693351308Illumina Small RNA Adapter 2 (100% over 21bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGGG1595560.15052375580261174No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAAG1570010.1481133908142962No Hit
TGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1561130.14727565926454111RNA PCR Primer, Index 1 (100% over 32bp)
GTGGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1551110.1463303810969121RNA PCR Primer, Index 2 (100% over 34bp)
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTTGGAATTCTCGGGTGCC1517440.14315398230409082No Hit
TGCGGCCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT1511670.142609645475027RNA PCR Primer, Index 7 (100% over 38bp)
AAGGTGAACAGCCTCTGGCATGTTGGAACTGGAATTCTCGGGTGCCAAGG1470180.13869551461924573Illumina Small RNA Adapter 2 (100% over 21bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCG1432500.13514081588109586No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG1416230.13360591809792977No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA1413680.13336535329479066No Hit
GGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGTGC1410790.1330927131845663No Hit
ACGAGCGTACGCGTTAGGACCCGAAAGATGGTGGAATTCTCGGGTGCCAA1401230.1321908310206408No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCAA1401140.13218234049817706No Hit
ATGGATTTTTGGAACTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1372000.12943329800269704RNA PCR Primer, Index 1 (100% over 31bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC1335100.1259521837925662No Hit
AATTCTCCAGGCATACGCGTATAACAACTCTGGAATTCTCGGGTGCCAAG1325920.12508615050126534No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAA1301890.12281918100344844No Hit
AGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACTGGAATTCTCGGG1287350.121447489929863No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA1267420.11956731089983841No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGTGGAATTCTCGGG1196150.1128437604999461No Hit
CATAATTTGTGGTAGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1190080.11227112192933651RNA PCR Primer, Index 1 (100% over 31bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATGGAATTCTCGGGTGCCAAG1181460.11145791855558779No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGAATGGAATTCTCGGGTGCCAAG1174620.11081263884834401No Hit
GCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCTGGAATTCTCGGGTGC1155520.10901076130326273No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1134640.10704096009167652RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1128160.10642964247428768No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGGT1119380.1056013448383812No Hit
GGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG1108370.10456267092365111RNA PCR Primer, Index 7 (100% over 36bp)
TGGGAAATGTGGCGTACGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA1106780.10441267169345848RNA PCR Primer, Index 1 (100% over 28bp)
ATAATTTGTGGTAGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1098060.10359003440586116RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC1065460.10051457849121982RNA PCR Primer, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)