FastQCFastQC Report
Mon 25 Sept 2023
SRR23433738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23433738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82561175
Total Bases12.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA30601123.7064782568804286RNA PCR Primer, Index 1 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA24358092.9503080594480395No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC22543162.730479550466669No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA19312302.3391503330712045No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC19281052.335365260971637No Hit
TGGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC12640991.5311058739171288RNA PCR Primer, Index 6 (100% over 35bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA12441661.506962564425712No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT11044941.3377886155326641No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG9698231.1746719932219958Illumina Small RNA Adapter 2 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC8955791.0847459474747059No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG8702701.0540911027489617No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGG8402661.0177495657008273No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA8371111.013928156909104No Hit
TGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG8203350.9936086786555545RNA PCR Primer, Index 6 (100% over 34bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG8114890.9828941993618671No Hit
GTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC7552120.914730198546714RNA PCR Primer, Index 1 (100% over 33bp)
GAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGT7385870.8945936149770154No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCG6907220.8366184226423619No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC6504480.7878376246462093No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC5728390.6938358132621053No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG5575610.6753307471702044Illumina Small RNA Adapter 2 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG5348500.6478226599851564No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT5273940.6387917807613566No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG4912900.5950617829748668No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA4433060.5369424550946615RNA PCR Primer, Index 1 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAG4332640.5247793530070278No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG4282310.5186832672863486Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGG4106800.4974250911521063Illumina Small RNA Adapter 2 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAAGGA4067210.4926298590106064RNA PCR Primer, Index 1 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAGG4033130.4885020107817022Illumina Small RNA Adapter 2 (100% over 21bp)
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGG3833150.46427997179061464No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC3774330.4571555576819249No Hit
ATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3763050.45578929805686513RNA PCR Primer, Index 1 (100% over 33bp)
ATGGATTTTTGGAAGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3555520.4306527856465221RNA PCR Primer, Index 1 (100% over 31bp)
GAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGC3520730.42643894057951576No Hit
TGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3463010.4194477610087308RNA PCR Primer, Index 1 (100% over 33bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAG3367550.40788542556474033No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC3323820.4025887470714897RNA PCR Primer, Index 1 (100% over 24bp)
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCTGGAATTCTCGGGTG3292420.39878550662584444No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG3275130.3966913019345958No Hit
GTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3157710.3824691206247973RNA PCR Primer, Index 1 (100% over 32bp)
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG3112830.37703315147828265Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA2958590.3583512468178899RNA PCR Primer, Index 1 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCAA2875860.3483307983443792No Hit
CGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT2805740.33983770216448594RNA PCR Primer, Index 6 (100% over 39bp)
TGGGAAATGTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC2724710.3300231616131917RNA PCR Primer, Index 6 (100% over 36bp)
GAGGTGGAGCACGAGCGTACGCGTTAGGACCCGATGGAATTCTCGGGTGC2720030.32945630921556046No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGA2506880.3036390894388312RNA PCR Primer, Index 1 (100% over 22bp)
CGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCAA2433100.29470268561463664No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGAA2230310.2701402929403561RNA PCR Primer, Index 1 (100% over 23bp)
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCTGGAATTCTCGGGTGC2175190.2634640313682551No Hit
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG2094820.25372943153970373No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG2083960.25241404328366207No Hit
TTTCGCCGAATCCCGGGGCCGAGGAATGGAATTCTCGGGTGCCAAGGAAC2062830.24985472893281863RNA PCR Primer, Index 1 (100% over 24bp)
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCTGGAATTCTCGGGT2002490.24254620891720594No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATGGAATTCTCGGGTGCCAA1983400.24023398407302224No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGGG1920080.2325645195819948No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGTGGAATTCTCGGGTGCCA1897190.2297920299705037No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGAAGTTGGAATTCTCGGGTGCCA1878920.2275791254182126No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGG1797360.21770039004410968No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCCA1750240.21199310692949802No Hit
CATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1677290.20315723462026794RNA PCR Primer, Index 1 (100% over 32bp)
TGCATAATTTGTGGTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1637160.19829659643288747RNA PCR Primer, Index 1 (100% over 30bp)
TGGCATGTTGGAACAATGTAGGTAAGGGAAGTGGAATTCTCGGGTGCCAA1582810.1917135990373199No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG1534000.1858016192235636No Hit
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA1524590.18466185831294188RNA PCR Primer, Index 6 (100% over 38bp)
AAGGTGAACAGCCTCTGGCATGTTGGAACTGGAATTCTCGGGTGCCAAGG1509900.18288257162037724Illumina Small RNA Adapter 2 (100% over 21bp)
ACCAGGAGTGGAGCCTGCGGCTTAATTTGTGGAATTCTCGGGTGCCAAGG1449130.17552196901267453Illumina Small RNA Adapter 2 (100% over 21bp)
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGATGGAATTCTCGGGTG1442520.17472135056217405No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCCA1392130.16861799750306364No Hit
GTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1389880.16834547231189478RNA PCR Primer, Index 1 (100% over 31bp)
GTGGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1332040.16133975806424752RNA PCR Primer, Index 6 (100% over 34bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCAA1305930.15817725462361698No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1302340.1577424255408187RNA PCR Primer, Index 1 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCCA1297380.15714165889717532No Hit
TGCGGCCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA1282700.15536358342768256RNA PCR Primer, Index 6 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1268360.1536266895426331No Hit
TGGGAAATGTGGCGTCCCGGAGTCGGGTTGCTGGAATTCTCGGGTGCCAA1253270.15179895392719397No Hit
TGGGAAATGTGGCGTCCCGGAGTCGGGTTTGGAATTCTCGGGTGCCAAGG1245870.15090264885401644Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGGGTGGAATTCTCGGGT1222240.14804052873520757No Hit
AATTCTCCAGGCATACGCGTATAACAACTCTGGAATTCTCGGGTGCCAAG1197540.1450488077477095No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGTGGAATTCTCGGGT1190840.14423728828956225No Hit
TGGGAAATGTGGCGTCCCGGAGTCGGGTTGCTTGGAATTCTCGGGTGCCA1190400.14418399447440033No Hit
AGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGTG1180790.1430200091023414No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA1169730.1416803963848625RNA PCR Primer, Index 1 (100% over 22bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCG1157760.14023056236784423No Hit
AATTCTCCAGGCATACGCGTATAACAACTGGAATTCTCGGGTGCCAAGGA1141350.13824294530691938RNA PCR Primer, Index 1 (100% over 22bp)
TGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1140840.13818117293025445RNA PCR Primer, Index 1 (100% over 32bp)
AGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACTGGAATTCTCGGG1132390.1371576894345314No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAAG1125870.1363679719916777No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTTGGAATTCTCGGGTGCC1054120.12767744645107096No Hit
CTAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1048600.1270088513154034RNA PCR Primer, Index 1 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCAA1012300.12261211156454593No Hit
TGCATGATGACGAACAAATACTGACTACCTGAAGATGGAATTCTCGGGTG990300.11994742080645049No Hit
ACGAGCGTACGCGTTAGGACCCGAAAGATGGTGGAATTCTCGGGTGCCAA974600.11804580058362785No Hit
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT970710.11757463480867368RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA970300.11752497466272736No Hit
ATGGATTTTTGGAACTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC954090.11556158206323978RNA PCR Primer, Index 1 (100% over 31bp)
GCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGTGCCAAGGA949520.11500805311939905RNA PCR Primer, Index 1 (100% over 22bp)
AATTCTCCAGGCATACGCGTATAACAACTCGGATTGGAATTCTCGGGTGC945100.11447269252163622No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG928010.11240271229182482No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGAATGGAATTCTCGGGTGCCAAG923370.11184070478648105No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCC910600.11029397292371385No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC901980.10924989863576919No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA889280.10771164533450497No Hit
AATGGATTTTTGGAAGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT884530.10713631437537074RNA PCR Primer, Index 1 (100% over 30bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGGT882030.10683350860740536No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC873680.10582213734240097RNA PCR Primer, Index 6 (100% over 50bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGG865110.10478411916981559No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA860790.1042608708027714No Hit
GGGAAATGTGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC860570.10423422389519044RNA PCR Primer, Index 6 (100% over 36bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGTGGAATTCTCGGGT836220.10128489571520755No Hit
GGTGGAGCACGAGCGTACGCGTTAGGACCCGAAATGGAATTCTCGGGTGC827610.10024203265033474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)