FastQCFastQC Report
Mon 25 Sept 2023
SRR23563662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23563662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97164691
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3722589638.3121642408146Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC37601073.8698285985389482Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC9047230.9311232204711072Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC8095810.8332049344962154Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT4495440.4626618943294946No Hit
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT4307380.44330712686566354Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2864080.2947655131224572Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC2085360.2146211734466381No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1911870.19676592189234668Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1741510.17923280381759255No Hit
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1741310.17921222020867641Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1730050.17805336302669866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1720410.17706123307694152Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1605740.165259620904882Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1522880.15673183173093197Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1483850.15271494045095044Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1461320.15039619690654912Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1431740.1473518811478544Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1429060.14707606078837837Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1327850.13665972549637398No Hit
TAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1269060.13060917365547944Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1197620.12325670855064007No Hit
TCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1159990.1193839025330714Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1125870.1158723388519807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1122730.11554917619199757Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1111440.11438723146868239Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1111190.11436150195753723Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1083930.11155595606226958No Hit
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1077920.11093741861434006Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1074390.11057411791697049No Hit
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1055090.10858779965656454Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1052900.108362409138933Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1044300.10747731395553968No Hit
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1031440.10615378790223293Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1025530.10554554225876148Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1021800.10516165795247576Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1009320.10387724075610966No Hit
TCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1007640.1037043384412142Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTGTAGGCACAATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1005120.10344498496887104Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1003830.10331222069136206Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT977410.10059312595354213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)