FastQCFastQC Report
Mon 25 Sept 2023
SRR23563663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23563663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92797251
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2038005421.961915660626627Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC20071012.16288842435645Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG6204980.6686598938151734Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT5813350.626457135028709Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC4887120.5266449110653073Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT3863930.41638410172301327No Hit
TGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3033280.3268717518367004Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2587530.2788369237360275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2464970.26562963594686656Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2446980.2636910009327755Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2439820.26291942635240345Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2416910.2604506032188389No Hit
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT2349590.2531960779743357Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT2253100.2427981406475069No Hit
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2156500.23238834952125897Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA2116980.22812960267540686No Hit
ATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2115960.22801968562624772Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2076000.22371352358271906Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2024030.218113142166248Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1932740.20827556626650504Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1842700.19857269263288843Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1745080.1880529844574814Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1742500.18777495898019653No Hit
TCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1725440.18593654245210345Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1716490.18497207422663847Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATC1690240.18214332663798413No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1668630.179814593861191No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1640950.17683174688008807No Hit
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1629150.1755601574878549Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1598140.17221846366979127Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1591690.17152339997657903Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1585350.17084019008278595No Hit
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1531760.1650652345294151Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1529720.16484540043109683Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1485160.16004353404822305Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1474530.1588980259770842Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1381770.14890204021237655Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1322130.14247512569095394No Hit
GAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1260670.13585208467005128Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1258670.13563656104424904No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA1245760.13424535603969562No Hit
CGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1225380.13204917029277086No Hit
CCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1190560.12829690396755394Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1183260.1275102427333758Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1177910.12693371703435483No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC1156220.1245963633125296No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1148020.12371271644674042No Hit
GACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1112510.11988609447062178Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1048220.11295808751920894No Hit
GAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1037870.1118427527556824Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1028370.11081901553312178Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1021680.1100980890048133Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1003470.10813574639188396No Hit
TAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC989220.10660014055804304No Hit
AGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA984450.10608611671050472Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG979110.10551066862961274No Hit
AATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC962960.1037703153512597Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA961130.10357311123365066Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG948350.10219591526477438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)