FastQCFastQC Report
Mon 25 Sept 2023
SRR23563664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23563664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126957333
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3236627225.493818462616886Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC33897592.66999858921107Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT8564010.6745581210342533No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG8032940.6327275321701977Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC6724840.5296929165958456Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT6237950.49134223700178076Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT4521790.3561661144850924Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3235090.25481710457796086No Hit
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2857780.2250976711995045Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2525680.19893927670960135Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2419840.19060261765265657Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2409410.1897810818064365Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2363800.18618853626989784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2357560.18569703256132516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2236300.17614579222454207No Hit
TGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2203190.17353782943754814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2127500.16757598397250517Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1950210.153611449919163Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1942040.15296792663406059Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC1939810.15279227707154183No Hit
CTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1921930.1513839299065931Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1915740.15089636452901858No Hit
AAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1915680.15089163853182075Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1914360.15078766659346884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1893740.14916349888981995Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1831930.1442949341098714No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1753590.13812435710192494No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1736200.1367546055807584No Hit
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1713590.13497369230338196Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1711050.13477362508867446No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1709940.13468619414051491No Hit
TAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1705530.13433883334647553Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1704280.13424037507152106No Hit
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1687520.13292024652093157Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1681750.13246576312374173No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1632580.12859280842013276No Hit
AGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1598880.1259383733273603Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1579140.12438352024927933Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGATTCCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1570720.12372030530918604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1563120.12312167899746287Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1548690.12198507667138848Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1546660.12182518043286243No Hit
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1540610.1213486423820828Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1502860.11837520247845787Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1493650.11764976190859334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1459150.11493231351985No Hit
AACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1452530.11441087849569115Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1427490.11243856233180323Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1418790.11175329273812014Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1415370.11148391089784472No Hit
AAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1404340.11061511507964648Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1394790.10986289385899435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1380910.10876961317389992No Hit
GAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1357480.10692411126815336Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1343580.10582925525065968No Hit
GTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1335490.10519203329515438Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1326710.10450046237187417No Hit
TCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1323730.10426573784438273Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1322090.10413656058764247No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT1311410.10329533308643148No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)