FastQCFastQC Report
Mon 25 Sept 2023
SRR23563665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23563665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136621629
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1958580514.335801105109061Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC16963811.2416635729032333Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT12965310.9489939546834126No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT6785720.49667977535240776Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT6734980.49296586853023106Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC6184970.45270796763812554Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT6054450.44315457547355114Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5838180.4273247246964095No Hit
ATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA5325780.38981968221151864Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA4644490.33995276106684397Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA4637600.33944844853225986Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG4285850.3137021591215253Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC4245510.3107494787666453No Hit
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG4136650.3027814871099217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA4097670.2999283517546113Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT4079310.29858449426042194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3898690.28536403997935056Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3892800.28493292229739114Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3679900.26934973817359475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC3612420.2644105495184807Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC3460800.2533127459635253Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3321940.24314890872806091No Hit
AGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3279730.24005935399877276Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3219070.23561935423855912Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3160780.23135282627906595Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3144360.23015096679896854No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3123610.22863217360700624No Hit
TGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3034550.2221134400322514Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTGGGGATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3009630.22028942430484416No Hit
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2959710.21663553726181964Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2733330.20006568652464243Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2638690.19313852567224182Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2579780.18882661690412136No Hit
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2577480.18865826874308458Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC2548410.18653049437728486Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG2526250.18490849644312177No Hit
GAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2496790.18275217608479838Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2447690.17915830882092615No Hit
AAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2370540.17351132594093135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATTCCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2295410.16801219666323844Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG2242970.16417385859159972No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT2240350.16398208807772305No Hit
GGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2232260.1633899417199893No Hit
GTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2197000.16080909121644274Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2192670.16049215750457785Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC2125920.15560640109187981Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2108740.15434891352378766No Hit
ATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2014630.14746054594327812No Hit
AGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1955080.14310179246947788Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1947830.14257112978794886Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1941750.14212610508399076Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1918750.1404426234736229Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1907010.13958331590380907Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1898740.13897799447260287No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC1890350.13836388965908172No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1884230.13791593716101863No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1874300.13718911227445546No Hit
GTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1869440.13683338529069947Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1748940.1280134055494244Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1747500.12790800496164484Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1743560.12761961724230356No Hit
GGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1708290.12503803479023076Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1698750.12433975589619121Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1692030.1238478864865533No Hit
ACGTGGGGATGGAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1679890.12295930097568958No Hit
GATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1672080.12238764917669076Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1658840.12141854932793987Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1654140.12107453352060384No Hit
CTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1639090.11997295098860226No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1629650.11928199157982518Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1577650.11547585924334133Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1534370.11230798602174477No Hit
CGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1533160.1122194202500689Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1524700.11160019179686402Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1524640.11159580010570655Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1520690.11130668043783903No Hit
AATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1518470.11114418786501222Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1517560.11107758054912373Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1502040.10994159643638857No Hit
CCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1488500.10895053813185028Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1480100.10833570136980286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAATGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1401030.10254818437276869Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT1392660.10193554345630002Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1369170.10021619636814608Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)