FastQCFastQC Report
Mon 25 Sept 2023
SRR23563666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23563666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128247336
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5336292741.6093843851852Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC33520922.6137712521373544Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC17704701.3805121066998227Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT10744040.8377593122090271No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC6829110.5324952714807268Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC5812940.45326009734814293No Hit
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG5156650.40208632481847423Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3454210.2693397077659375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3401610.26523825804849466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3211950.25044964676693166No Hit
TGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3091740.24107635265031938Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2892360.225529830888651No Hit
AAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2878210.22442649413006133No Hit
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC2857090.22277967629674586Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2777540.21657681840658274Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2674840.2085688547947694Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGGAACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2472630.19280166568138304Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2267000.17676780436203368Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG2211960.17247609728127217No Hit
CTGTAGGCACAATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2203490.17181565471270296Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGA2181110.17007058922455903No Hit
CTGTAGGCACCATCAATAGATAGGAAGAGCACACGTCTGAACTCCAGTCA2134910.16646817521418145Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1943550.15154700757292922Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGGCACCATAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1857590.14484433423240853Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATAGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1831770.142831037051717No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1813730.14142438015242673No Hit
CTGTAGGCAACATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1813450.14140254734024263Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1753230.13670693323407512No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1707060.13310685845357445No Hit
AGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1696910.13231541901190055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1598110.12461155528408012No Hit
TCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1489860.1161708341450461Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1311140.10223526202524783No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)