FastQCFastQC Report
Mon 25 Sept 2023
SRR23563667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23563667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108592981
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3057312528.153868434645883Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC33706133.103895821775074Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC10070160.9273306531662483Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA8491830.7819870052190574Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT7606910.7004973921841229Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGCATGATGAGTAATAGAAAGGAGCTTCTGTAGGCACCATCAATAGAT6362050.5858619904724781No Hit
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC4270300.3932390436910467Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT3471580.31968732859446963Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2991350.27546439672744594No Hit
CTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2823320.25999102096663135Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2273920.20939843248248247Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2252800.20745355540060184Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2243340.20658241254100945Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2144690.19749803166375918Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2112500.194533751679586Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT2076860.1912517716039124Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA2054680.18920928232000556Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1997740.1839658495055035No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1995470.18375681205399452Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1936140.17829329135001828Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1922530.177039987510795Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1916380.17647365256507694Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1878400.17297618894908134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1853100.1706463882780785Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAAGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1763530.16239815720686404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG1751690.16130784732762793Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1727960.1591226232200035Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1724980.15884820401053362No Hit
AACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1699000.15645578419106113Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1688880.15552386392266Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1674670.15421530789361054No Hit
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1650060.15194904724090777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1644730.15145822362128544Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1637240.15076849211828894Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1568590.1444467207323464No Hit
TCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1536600.14150085814478192Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1446670.13321947575967177Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1410350.12987487653552857Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1368680.12603761195210214No Hit
GAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1300560.11976464666717272Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1300000.11971307795666832No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1292280.11900216644757178No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1276090.11751127819209603No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1261130.11613365692576393Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1235700.1137918849469654Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1233760.11361323619986084No Hit
AAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1220670.11240781759182022Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1187560.10935881758324693No Hit
GAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1159140.10674170552514807Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1151320.1060215853177472Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA1138920.10487970672800667Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1112060.10240625036345581Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)