FastQCFastQC Report
Mon 25 Sept 2023
SRR23678239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23678239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48693546
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length16-89
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTTCTGGCGCCAAGCGCCCGGCC11400572.3412897471052942No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG9648671.9815090073744064No Hit
TTCGGTTGGCCTCGGATAGCCGGTC8235971.6913884234267924No Hit
ATTGATCATCGACACTTCGAACGCA5939061.2196811462447201No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC5019901.0309169104258704No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC3978220.8169912291867181No Hit
TCGCTTCTGGCGCCAAGCGCCCGG3901410.8012170647830823No Hit
TCGGTTGGCCTCGGATAGCCGGTC3869280.79461865439005No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC3691500.7581086824114226No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC3609360.7412399170929141No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG3156420.648221429591511No Hit
TCAGTGGCTGGTTGAGAT2644630.5431171515009402No Hit
AGCAGTGGCTGGTTGAGATTT2643550.5428953561936113No Hit
TAGCAGTGGCTGGTTGAGATTT2472530.5077736585460423No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCC2204640.45275815402722985No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCG2172560.4461700119354627No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2077430.42663354194824915No Hit
AGCGAGCGCACGGGGTCGGCGGCGA2057250.42248925555760514No Hit
CGGTTGGCCTCGGATAGCCGGTC2054070.4218361915971369No Hit
AGAGCAGTGGCTGGTTGAGATTTAA2029120.41671230926579056No Hit
CTCGGTTGGCCTCGGATAGCCGGTC1967290.4040145279212157No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1873530.38475940938866926No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT1847930.37950203914087505No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT1769680.3634321476608009No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGAG1635890.33595622713531686No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC1501440.3083447650331319No Hit
TGCGAGCGCACGGGGTCGGCGGCGA1433940.2944825583250807No Hit
TCGTCCGACCTGGGTATAGGG1417760.2911597360356545No Hit
ATTGATCATCGACACTTCGAACGCAC1402490.2880237968292554No Hit
CGCTTCTGGCGCCAAGCGCCCGG1399120.28733171332397933No Hit
CCTCGGTTGGCCTCGGATAGCCGGTC1379530.28330859288826493No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTC1362450.2798009411760647No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCG1343530.2759154159773043No Hit
TATTGATCATCGACACTTCGAACGCA1340500.2752931569206317No Hit
TTTGATCATCGACACTTCGAACGCA1327370.2725967010083841No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1324910.2720915005861352No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC1282530.26338808843373207No Hit
CCGGGGTCCGGTGCGGAGTGCCCT1275620.2619690091988782No Hit
TTTCACTGACCCGGTGAGGCGGGG1241170.2548941496271395No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGA1194800.2453713270337716No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCC1191850.24476549725912342No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCG1187020.24377357935690286No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC1134300.2329466825028516No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCG1121550.23032826568021972No Hit
CGCTTCTGGCGCCAAGCGCCCGGC1099780.22585744730934157No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG1098450.22558431049568667No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT1097730.22543644695746742No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC1082830.22237649318043096No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA1079950.2217850390275541No Hit
TCGCTTCTGGCGCCAAGCGCCCG1060210.21773111368804401No Hit
TCTCGGTTGGCCTCGGATAGCCGGTC1026120.21073018588541487No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1017620.20898457467032694No Hit
TCTAAACCATTCGTAGACGACCTGCTT1000150.205396830208258No Hit
TCGGGGTCCGGTGCGGAGTGCCCTT985740.20243750578362069No Hit
TAGAGCAGTGGCTGGTTGAGATTTAA985100.20230607152742583No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC978340.20091779719636765No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC937830.1925984195112839No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG892210.18322962143689434No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTC877520.18021279452517178No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC865190.17768063143316776No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA860630.1767441623577794No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC839460.17239656360208394No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCC837490.17199199253223413No Hit
TCATCTGTGGGATTATGACTGAAC817330.16785181346209618No Hit
CCGCCACGCAGTTTTATCCGGTAA809080.16615754375333439No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA808780.16609593394574304No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCG798640.1640135224491558No Hit
TCAGTGGCTGGTTGAGATTT781960.16058801714707735No Hit
TCCGGGGTCCGGTGCGGAGTGCCCTT774510.1590580402585591No Hit
TTTTCACTGACCCGGTGAGGCGGGG749130.15384585053633187No Hit
AGCGAGCGCACGGGGTCGGCGGCGAC747280.15346592338951862No Hit
CAGTGGCTGGTTGAGAT746500.1533057378897811No Hit
TCGGTTGGCCTCGGATAGCCGGTCCC746460.15329752324876894No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT745470.15309421088371752No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG745240.1530469766978975No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC742420.1524678445065389No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG739000.15176549269999765No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAG722110.1482968605326053No Hit
CCGCCACGCAGTTTTATCCGGTAAAGC704920.14476661855762157No Hit
CCGCCACGCAGTTTTATCCGGTAAA702970.14436615480827789No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTT700550.14386916902704106No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGA694540.14263491921496124No Hit
GCGAGCGCACGGGGTCGGCGGCGA686180.14091805924341597No Hit
ATGTTAGGGATAACAGGGTAATGCGA677650.1391662870475689No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGA675830.13879252088151475No Hit
ACCTCTAGCATCACCAGTATTAGAGGC668270.13723995373021303No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTC651780.13385346797294245No Hit
TCGGGGTCCGGTGCGGAGTGCCCTTC649140.13331130166613867No Hit
TAGCGAGCGCACGGGGTCGGCGGCGA647960.1330689697562794No Hit
TCGAGCGCACGGGGTCGGCGGCGA617420.12679709134348113No Hit
TCACTGACCCGGTGAGGCGGGG613700.12603312972934855No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA601820.1235933813487315No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC594650.1221209069472985No Hit
TGTGGGGGGGCCGGGCCA594030.12199358001160976No Hit
TCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG590180.12120292081418758No Hit
CTAAACCATTCGTAGACGACCTGCTT586480.12044306652056107No Hit
GGTTGGCCTCGGATAGCCGGTC585860.1203157395848723No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT585320.12020484193120788No Hit
CCATCTGTGGGATTATGACTGAAC570420.1171448881541714No Hit
AATTGATCATCGACACTTCGAACGCA565090.11605028723929861No Hit
TTCGGTTGGCCTCGGATAGCCGGT561300.1152719500033947No Hit
TCCGGGGTCCGGTGCGGAGTGCCCT555260.11403153921055575No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC550100.11297185051998473No Hit
TTCGGTTGGCCTCGGATAGCCGGTCC543130.11154044932361262No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC537410.11036575565887109No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGC535940.11006386760167354No Hit
CCCCCGAGAGAGGGGCCCGTGCCT522850.10737562633043812No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCT512250.10519874646221082No Hit
TACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC512010.10514945861613775No Hit
CGGTTGGCCTCGGATAGCCGGTCCCC504050.10351474505471422No Hit
AAAAGCATCGCGAAGGCCCGC496020.10186565587151941No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCG491160.10086757698853971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)