FastQCFastQC Report
Mon 25 Sept 2023
SRR23678240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23678240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71587924
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length16-89
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTTCTGGCGCCAAGCGCCCGGCCG18775742.622752407235611No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC14600342.03949761135691No Hit
ATTGATCATCGACACTTCGAACGCA13223421.8471579089232983No Hit
TTCGGTTGGCCTCGGATAGCCGGTC12580091.757292193582817No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC7597331.061258599983986No Hit
TCGGTTGGCCTCGGATAGCCGGTC6817280.9522946914901458No Hit
TCGCTTCTGGCGCCAAGCGCCCGG6567420.9173921568112522No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC6559140.91623553715568No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG5526880.7720408263270773No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC5473170.7645381642859207No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG5171190.7223550720649478No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC4316100.602908948721575No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC3979890.5559443237940522No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCC3820930.5337394614208955No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCG3800700.5309135658131391No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT3186750.44515189461284005No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT3101760.4332797805395223No Hit
CGGTTGGCCTCGGATAGCCGGTC3091430.4318367997373411No Hit
TATTGATCATCGACACTTCGAACGCA3063220.42789619098327253No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC3037030.424237752724887No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2938260.41044073299289974No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTC2914710.4071510720160009No Hit
CTCGGTTGGCCTCGGATAGCCGGTC2891220.40386979234095405No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC2857280.39912876926002216No Hit
TTTGATCATCGACACTTCGAACGCA2730850.381467969374276No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT2599130.36306821804191447No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG2587900.36149951771195377No Hit
ATTGATCATCGACACTTCGAACGCAC2458500.3434238433845351No Hit
TCGTCCGACCTGGGTATAGGG2422170.3383489651131663No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC2377870.3321607705791273No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCG2281840.31874649696504676No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC2237310.31252617410724187No Hit
CCTCGGTTGGCCTCGGATAGCCGGTC2190590.30599993373184003No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG2106770.29429125504463577No Hit
TCTCGGTTGGCCTCGGATAGCCGGTC2089870.29193052168966377No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA2067100.28874981763684054No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCC2061590.28798013475010115No Hit
CGCTTCTGGCGCCAAGCGCCCGG2051980.2866377295701437No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT1995160.2787006367163266No Hit
AGCGAGCGCACGGGGTCGGCGGCGA1978300.27634549089592264No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG1972340.27551294824529343No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGAG1954650.2730418610826038No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1948780.2722218903847526No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTC1892340.2643378791093313No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT1883660.26312538410807945No Hit
TTTCACTGACCCGGTGAGGCGGGG1845760.2578311951049174No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA1794420.2506595944869137No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCG1773450.24773032948964968No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1769400.24716459161464158No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC1686770.23562214208083473No Hit
CGCTTCTGGCGCCAAGCGCCCGGC1686340.23556207608422894No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC1677670.23435097796661908No Hit
TCTAAACCATTCGTAGACGACCTGCTT1647360.23011702364773143No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC1628450.2274755166807184No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG1612020.22518043685692016No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT1526570.21324406613607064No Hit
CCGGGGTCCGGTGCGGAGTGCCCT1451350.2027367073809823No Hit
TCCGGGGTCCGGTGCGGAGTGCCCTT1447820.20224360745535797No Hit
TCGGTTGGCCTCGGATAGCCGGTCCC1415330.19770513250251537No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGC1342600.18754559777428384No Hit
TTTTCACTGACCCGGTGAGGCGGGG1319780.18435790930325066No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1300670.1816884646633977No Hit
TGCGAGCGCACGGGGTCGGCGGCGA1284920.17948837292725517No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1283860.17934030326120365No Hit
TCATCTGTGGGATTATGACTGAAC1279340.1787089118550218No Hit
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC1236650.17274561558734403No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGA1201910.16789284181505248No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTC1194680.16688289494188993No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCG1193490.16671666578849248No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC1193040.1666538060246027No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC1172380.1637678444202405No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC1165820.1628514887510916No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTT1156870.16160127789150583No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCG1151120.16079806979735856No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAG1147950.16035525768284606No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1133940.1583982237004107No Hit
ACCTCTAGCATCACCAGTATTAGAGGC1085450.15162473492037568No Hit
CCATCTGTGGGATTATGACTGAAC1070680.14956153778114867No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1062280.14838815552187265No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC1055630.1474592278999458No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCC1043410.1457522360894276No Hit
TCGCTTCTGGCGCCAAGCGCCCG1012440.14142608745016827No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC988220.13804283526925573No Hit
CCGCCACGCAGTTTTATCCGGTAAA987800.13798416615629194No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC972110.13579245572200138No Hit
CCGCCACGCAGTTTTATCCGGTAAAGC970440.13555917615378818No Hit
CTAAACCATTCGTAGACGACCTGCTT966870.13506048869359588No Hit
AATTGATCATCGACACTTCGAACGCA960140.13412038600253304No Hit
TTTGGAACAATGTAGGTAAGGG959240.1339946664747535No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT952680.1330783108056046No Hit
CCGCCACGCAGTTTTATCCGGTAA952150.13300427597257883No Hit
AGCGAGCGCACGGGGTCGGCGGCGAC942930.13171634925465922No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC915140.12783440961355438No Hit
TGCCTCGGTTGGCCTCGGATAGCCGGTCCCC913830.127651417856453No Hit
TTGATCATCGACACTTCGAACGCA908940.1269683417555173No Hit
GTTGGCCTCGGATAGCCGGTCCCC894290.12492190721999426No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG894000.12488139759437639No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG887240.12393710425238759No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGA886740.12386726007028784No Hit
TCCGGGGTCCGGTGCGGAGTGCCCT884430.12354457994898692No Hit
TTCGGTTGGCCTCGGATAGCCGGTCC882480.12327218763879784No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG878190.12267292455638189No Hit
TCATTGATCATCGACACTTCGAACGCA874960.12222173114001741No Hit
TGTGGGGGGGCCGGGCCA861830.12038762291807764No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC843940.11788859808254812No Hit
TCGCGCTACACTGACTGGCTCAGCG839460.11726279421093425No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCG836230.11681160079456976No Hit
CGGTTGGCCTCGGATAGCCGGTCCCC819110.11442013599947387No Hit
GGTTGGCCTCGGATAGCCGGTC804120.11232620742012299No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCT784000.10951567753242851No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGAT763070.10659200006973243No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG761440.10636430803608721No Hit
CCCCCGAGAGAGGGGCCCGTGCCT760620.10624976357744359No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTC760370.10621484148639372No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGAT751600.10498977453236387No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACA750160.10478862328791655No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA744810.10404129053944908No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT729560.1019110429854063No Hit

[OK]Adapter Content

Adapter graph