FastQCFastQC Report
Mon 25 Sept 2023
SRR23678241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23678241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156010751
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length16-89
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTTCTGGCGCCAAGCGCCCGGCCG28846691.849019366620445No Hit
TTCGGTTGGCCTCGGATAGCCGGTC25033001.6045689056390735No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC24664641.580957712330992No Hit
ATTGATCATCGACACTTCGAACGCA21243901.3616946180843652No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC20558801.3177809777994083No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC16789151.0761533991974694No Hit
TCGGTTGGCCTCGGATAGCCGGTC12998100.8331541202567507No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG10919820.6999402239913581No Hit
TCGCTTCTGGCGCCAAGCGCCCGG10907060.699122331639824No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC10289470.6595359572366907No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC10055670.6445498105447873No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCG9622370.6167760835918289No Hit
AGCGAGCGCACGGGGTCGGCGGCGA8938000.5729092349539423No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC8921620.5718593073114557No Hit
CGGTTGGCCTCGGATAGCCGGTC7603770.48738756471981853No Hit
TGCGAGCGCACGGGGTCGGCGGCGA7549880.4839333155956669No Hit
CTCGGTTGGCCTCGGATAGCCGGTC6932620.4443680935809353No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT6626290.4247329083109151No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCG6133730.3931607251861764No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTC6074110.3893391936815944No Hit
CCTCGGTTGGCCTCGGATAGCCGGTC6017990.38574200569036426No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA5653870.3624025885241717No Hit
TTTGATCATCGACACTTCGAACGCA5367990.3440782103535929No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGAG5260610.33719535136395823No Hit
ATTGATCATCGACACTTCGAACGCAC5133470.32904591299608577No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCG5129280.32877734176153023No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCG4992450.32000679235240653No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG4953170.3174890171511321No Hit
TATTGATCATCGACACTTCGAACGCA4904760.3143860258707427No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCC4701880.3013817938739363No Hit
TTTCACTGACCCGGTGAGGCGGGG4529520.290333837313558No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGA4496930.2882448787135189No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT4320120.27691168540045036No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG4243940.27202868858698076No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT3919250.2512166613440634No Hit
TCGTCCGACCTGGGTATAGGG3894450.2496270273065989No Hit
CCGGGGTCCGGTGCGGAGTGCCCT3861660.24752524907722548No Hit
CGCTTCTGGCGCCAAGCGCCCGG3840300.2461561126643125No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA3806640.24399856904733444No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTC3767900.24151540684526288No Hit
TCTCGGTTGGCCTCGGATAGCCGGTC3602830.2309347257741231No Hit
TCTAAACCATTCGTAGACGACCTGCTT3582630.2296399432113496No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC3581000.2295354632322743No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTC3568300.22872141676954044No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC3489210.22365189434925548No Hit
GCGAGCGCACGGGGTCGGCGGCGA3475600.22277951857305012No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC3320450.21283469111689615No Hit
AGCGAGCGCACGGGGTCGGCGGCGAC3308420.21206359041243253No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG3242930.20786580278688616No Hit
CCGCCACGCAGTTTTATCCGGTAA3225260.20673318853519268No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA3209640.20573197548417674No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT3178380.20372826741921138No Hit
CGCTTCTGGCGCCAAGCGCCCGGC3154780.20221555115775325No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC3128460.20052848793734734No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG3107510.19918563176456985No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCG3058380.19603648981857666No Hit
CCGCCACGCAGTTTTATCCGGTAAA3037900.19472375977473502No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCC3029690.19419751399055823No Hit
CCGCCACGCAGTTTTATCCGGTAAAGC2954610.1893850251384278No Hit
GTTGGCCTCGGATAGCCGGTC2903870.18613268517629275No Hit
TCGAGCGCACGGGGTCGGCGGCGA2861870.18344056301607062No Hit
TGTGGGGGGGCCGGGCCA2796150.17922803281678965No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGA2761060.1769788288500707No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC2745190.1759615912623868No Hit
TCGGGGTCCGGTGCGGAGTGCCCTT2708170.17358867787259097No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2661850.17061965171874596No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCG2616560.16771664665597308No Hit
TCATCTGTGGGATTATGACTGAAC2589100.1659565115483612No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC2572780.16491042979467485No Hit
TTTTCACTGACCCGGTGAGGCGGGG2528610.16207921465617456No Hit
TGCGAGCGCACGGGGTCGGCGGCGAC2521030.16159335070440115No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA2486060.15935183851528284No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC2480300.15898263319045236No Hit
TCACTGACCCGGTGAGGCGGGG2476210.15872047177056406No Hit
TAGCGAGCGCACGGGGTCGGCGGCGA2459590.15765516057287618No Hit
GGTTGGCCTCGGATAGCCGGTC2431520.15585592559579436No Hit
TCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG2404950.1541528378387205No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC2380740.15260102170779244No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGA2324950.149024986104964No Hit
TCGCTTCTGGCGCCAAGCGCCCG2244760.14388495572333987No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG2179850.1397243450228632No Hit
AATTGATCATCGACACTTCGAACGCA2167760.13894939842959925No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2144740.13747385909321083No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC2087250.13378885664104007No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT2086290.133727322420235No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCG2050040.13140376460337658No Hit
CTAAACCATTCGTAGACGACCTGCTT2029380.13007949689313397No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAG2022690.1296506802918986No Hit
TGAGGGCGCACCACCGGCCCGTCTCGC1956500.12540802396368184No Hit
TCCGGGGTCCGGTGCGGAGTGCCCTT1954120.12525547037460258No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1943380.12456705627934578No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTT1911890.12254860564064587No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT1911770.12254091386304523No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG1903970.12204094831900399No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC1899120.121730072307645No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGC1895280.12148393542442469No Hit
TCGCGCTACACTGACTGGCTCAGCG1886990.12095256178851418No Hit
TCGGTTGGCCTCGGATAGCCGGTCCC1852530.11874373965419859No Hit
TACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC1850820.11863413182338953No Hit
TCGGGGTCCGGTGCGGAGTGCCCT1829230.11725025283674201No Hit
TGAGGGCGCACCACCGGCCCGTCTCG1810300.11603687492024188No Hit
CCATCTGTGGGATTATGACTGAAC1805510.1157298447976832No Hit
TCCGGGGTCCGGTGCGGAGTGCCCT1790570.11477221848640419No Hit
TAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTC1788750.11465555985946123No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACA1766410.11322360726280974No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC1763610.11304413245212824No Hit
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC1755680.11253583414901965No Hit
CCCCCGCCACGCAGTTTTATCCGGTAA1753590.11240186902247525No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1740570.11156731115280638No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG1740330.11155192759760511No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG1737370.11136219708345614No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG1734520.11117951736544106No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGC1726340.11065519452566445No Hit
ACCTCTAGCATCACCAGTATTAGAGGC1689680.10830535646867054No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1683720.1079233315145057No Hit
CCGGCCGAGGTGGGATCCCG1683450.10790602501490427No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGC1683030.10787910379330204No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1672450.10720094540151275No Hit
TTGATCATCGACACTTCGAACGCA1669280.10699775427656265No Hit
TGCCTCGGTTGGCCTCGGATAGCCGGTC1666830.10684071381721635No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTT1637020.10492994806492535No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAA1626940.10428383874647204No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCT1617600.10368516205655595No Hit
TTTGATCATCGACACTTCGAACGCAC1612970.1033883876374648No Hit
AGCGAGCGCACGGGGTCGGCGGCG1595260.10225320945990446No Hit
TTCGGTTGGCCTCGGATAGCCGGT1570520.10066742131124026No Hit
TTTGGAACAATGTAGGTAAGGG1566880.10043410405735437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)