FastQCFastQC Report
Mon 25 Sept 2023
SRR23678242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23678242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28292863
Total Bases673.5 Mbp
Sequences flagged as poor quality0
Sequence length16-89
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGGGGGCCGGGCCACCCCTCCCACGGC3993941.4116422222805802No Hit
TGTGGGGGGGCCGGGCCACCCCTCC2430640.8591000493658065No Hit
TTCGGTTGGCCTCGGATAGCCGGT2119050.7489698020309927No Hit
AGAATCCCGCCCAGGCGGAACGAT1930170.6822109165834508No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT1616670.5714055873384041No Hit
CCCCCGCCCCTTGCCTCTCGGC1567190.5539170779570806No Hit
AGAATCCCGCCCAGGCGGAAC1513500.534940560805034No Hit
TCTCCCGGGGCTACGCCTGT1493840.5279918119279763No Hit
CTCGGTTGGCCTCGGATAGCCGGT1491310.5270975934814374No Hit
AAGTCCTTCTGATCGAGGCC1439910.5089304677296178No Hit
TCGGTTGGCCTCGGATAGCCGGT1404680.49647856422306924No Hit
AAAAGCATCGCGAAGGCCCGC1390200.4913606657622454No Hit
TCCTCCCGGGGCTACGCCTGT1033810.36539603644919216No Hit
TAGCCTCGGTTGGCCTCGGAT1021790.36114761521306626No Hit
TCTCGGTTGGCCTCGGATAGCCGGT1016850.3594015918431443No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTT991540.3504558729174916No Hit
TTGGGGGGGCCGGGCCACCCCTCC967980.3421286845378639No Hit
CCTCCCGGGGCTACGCCTGT940550.33243366003645514No Hit
ACGCCTGTCTGAGCGTCGCT884760.31271490622917875No Hit
TTCGGTTGGCCTCGGATAGCCGGTC876160.3096752704029988No Hit
AAAGTCCTTCTGATCGAGGCC865420.30587926008053695No Hit
TCGGGACCGGGGTCCGGTGCGGAGT857320.303016347267507No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGT837860.2961382875957092No Hit
TGTGGGGGGGCCGGGCCACCCCTC834040.29478812377524327No Hit
CGGTTGGCCTCGGATAGCCGGT834020.29478105485471723No Hit
TCACCCCTCCCACGGC787460.27832460787018976No Hit
CCCCCGCCCCTTGCCTCTCGG747380.26415849113608614No Hit
CTCGGTTGGCCTCGGATAGCCGGTC746170.2637308214442632No Hit
TAGAATCCCGCCCAGGCGGAAC654230.23123499378624213No Hit
ACCCCTCCCACGGCGCGACC652810.23073310042889617No Hit
TAGAATCCCGCCCAGGCGGAACGAT638980.2258449418851673No Hit
AGATCCCCGAATCCGGAGT629160.2223741019069014No Hit
CCCCCGCCCCTTGCCTCT595430.21045236743980275No Hit
TAAGTCCTTCTGATCGAGGCC570240.20154906203730602No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG560870.1982372727708751No Hit
CTCCCGGGGCTACGCCTGT551720.1950032416302302No Hit
TCGCCGGCCGAGGTGGGAT524670.1854425266188155No Hit
AAGAGGGACGGCCGGGGGC518720.18333952276233056No Hit
TCGGTTGGCCTCGGATAGCCGGTC513930.18164651629635362No Hit
AAAAGGACCTGGCGGTGCTTC509720.18015850852563065No Hit
TCGGGTCGCGCCGTCGGGCCCGGGGG507660.1794304097114527No Hit
TGGCCTCGGATAGCCGGTCCCC495680.17519612631637882No Hit
CCTCGGTTGGCCTCGGATAGCCGGT483300.17082046451078495No Hit
TCGGGGCTACGCCTGT474700.16778082868460503No Hit
CCTTCTGATCGAGGCC472740.16708807447305704No Hit
TGTGGGGGGGCCGGGCCACCCCTCCC471300.16657911219518506No Hit
ACGCCTGTCTGAGCGTCGCTT462070.16331680537243615No Hit
ACTCGCCGAATCCCGGGGC456440.16132690424436721No Hit
TCTCGGTTGGCCTCGGATAGCCGGTC454570.16066596017518625No Hit
TCGCGGGTCGGGGGGCGGGGC446480.1578065818224193No Hit
ACCACCGGCCCGTCTCGC444190.15699719042219235No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGC430630.15220446230556448No Hit
AAAACGATGCCGACCGGC430180.15204541159372947No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC428300.1513809330642855No Hit
CCCCCGCCCCTTGCCTCTC428170.1513349850808665No Hit
ATTGATCATCGACACTTCGAACGCA417850.14768742208945063No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGC413510.14615346633530868No Hit
TAACCTCGGGCCGATCGCAC401480.1419015106389198No Hit
TCGGATAGCCGGTCCCCCG398710.14092246514606882No Hit
ATGTTAGGGATAACAGGGT392930.1388795471140549No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG385160.13613327148970397No Hit
ACTGACCCGGTGAGGCGGGG379190.13402319871269303No Hit
ACCCCTCCCACGGCGCGACCGC377610.13346475399113902No Hit
CCCCCCGCGGGGGCGCGCCGGC375420.13269070719354206No Hit
CCGGTGAGCTCTCGCTGGC370610.13099063180703913No Hit
CGTGGGGGGCCCAAGTCCTT366610.12957684770183914No Hit
TGAGGGCGCACCACCGGCCCGT365300.12911383340738616No Hit
ACCCCTCCCACGGCGCGACCGCT365130.12905374758291516No Hit
AGCGCCGCGGAGCCTCGGTTGGC363810.1285871988281992No Hit
CGCGGGTCGGGGGGCGGGGC355760.12574195831648427No Hit
AGCGCCGCGGAGCCTCGGTTGGCCT355220.12555109746228227No Hit
TGTGGGGGGCCCAAGT336210.11883208850231948No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGT329040.11629788049374855No Hit
TGATGCCGACCGGCGATGCGGCGGCGTT326660.11545667895115456No Hit
TCTTCTGATCGAGGCC325850.11517038766985159No Hit
CCCTCCCGGGGCTACGCCTGT322790.11408884282937361No Hit
TGAGCCTCGGTTGGCCTCGGAT317330.11215902752577567No Hit
TGTGGGGGGCCCAAGTCCTT312050.11029283250691173No Hit
AGGGCACCACCAGGAGTGGAGCCTGC309680.10945516542458075No Hit
TGTGGGATTATGACTGAAC306640.10838068950462879No Hit
CCTCGGTTGGCCTCGGATAGCCGGTC302600.10695276755837682No Hit
CGTGGGGGGCCCAAGT302160.10679725130680483No Hit
ACCCCTCCCACGGCGCGACCGCTC300770.10630596133024783No Hit
AAATGTAGGTAAGGGAAGT296370.1047507988145279No Hit
TCGGACCAGGGGAATCCGACT295270.10436200818559792No Hit
TCGGGGCTACGCCTGTCTGAGC292560.10340416945432494No Hit
TCTCGGATAGCCGGTCCCC290030.10250995100778595No Hit
AGGACCCGAAAGATGGTGAACT285770.10100427093574799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)