FastQCFastQC Report
Mon 25 Sept 2023
SRR23678244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR23678244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141255854
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length16-89
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT72940135.163689003642992No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG46049993.2600411732316594No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC26120841.8491863707114042No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG17127721.2125316944386602No Hit
ATTGATCATCGACACTTCGAACGCA15424681.0919674875917No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT14386301.0184569058638802No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT14117830.9994509678869662No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC12902520.9134148875698985No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC12859210.9103488199504992No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG11985270.8484795256697821No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT10778280.7630324474906364No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC10115710.7161267808412386No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC9447580.6688275021862102No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC7162080.5070288980731376No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG6795680.48109015007618733No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCC6697190.47411769568148304No Hit
AATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT6483640.45899973816306405No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT6271900.44400991692705355No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC6059310.42895992119377935No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC5942310.42067707862925097No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC5660700.40074091371816706No Hit
ATTGATCATCGACACTTCGAACGCAC5646860.3997611313156621No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC5559640.39358651996114796No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG5172800.36620075228882193No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG4665630.33029639960974644No Hit
TCGCTTCTGGCGCCAAGCGCCCGG4427770.31345745146958653No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTG4425460.3132939184240817No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC4410600.31224192662486044No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC4349410.30791007075713833No Hit
TCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT4329510.3065012795859066No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCG4290260.303722633682849No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG4255490.301261142777134No Hit
TATTGATCATCGACACTTCGAACGCA4177450.29573641599306744No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC3966120.2807756201027959No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3935340.2785965953665892No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC3919380.2774667306885561No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC3751070.265551472295088No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3688310.26110847059124365No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG3220120.22796364956315368No Hit
TGCCTCGGTTGGCCTCGGATAGCCGGTCCCC3200580.22658034406135125No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCCG3177800.22496766753468495No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC3121690.22099544278002098No Hit
TCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3094160.21904649700394008No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC2972690.21044720737733108No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG2915170.20637516375073559No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGC2872470.20335228018231372No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC2861720.2025912497757438No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCC2859190.20241214215447664No Hit
TTTGATCATCGACACTTCGAACGCA2803720.19848522525657591No Hit
TCATTGATCATCGACACTTCGAACGCA2781160.19688812330567199No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA2739240.1939204586876803No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC2737810.19381922394522494No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2654840.18794548507702907No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA2602500.18424015191611104No Hit
ACGATGCCGACCGGCGATGCGGCGGCG2602490.1842394439808491No Hit
TAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC2590250.1833729312202523No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG2565830.18164415331063022No Hit
TCGTCCGACCTGGGTATAGGG2549960.18052066004995446No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCG2532230.17926549083056054No Hit
AGAGCAGTGGCTGGTTGAGATTTAA2515350.1780704961084303No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTC2514110.17798271213595154No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2494660.17660577805150646No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTC2440100.17274328326244093No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCC2403190.1701302942106739No Hit
AGCAGTGGCTGGTTGAGATTT2393270.16942802243084382No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT2378350.16837178302005099No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTC2352610.16654955765585475No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT2315230.16390329564677722No Hit
TTCGGTTGGCCTCGGATAGCCGGTC2304760.16316208742754124No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC2272320.16086554543785492No Hit
TCATCTGTGGGATTATGACTGAAC2265900.16041105099969874No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCC2263680.16025388937155127No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT2243560.15882952362455718No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCG2236180.15830706740125616No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCC2190490.15507251118951856No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCG2186040.15475747999796172No Hit
TAGCAGTGGCTGGTTGAGATTT2182650.15451748994416897No Hit
AATTGATCATCGACACTTCGAACGCA2169060.1535554059232122No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTT1991690.14099875818243965No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1985760.14057895257211783No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCC1984720.14050532730487758No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCC1972550.13964377009111426No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT1951800.13817480442261884No Hit
CCCCCGCCCCTTGCCTCTCGGC1947760.13788879857680095No Hit
CCCCCGCCCCTTGCCTCTCGG1947470.13786826845420508No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGC1943510.1375879260904826No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA1934490.1369493684842258No Hit
TGTGGGGGGGCCGGGCCA1907690.13505210198226544No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG1895450.13418558922166865No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCC1885160.1334571238371473No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA1835550.1299450570027349No Hit
CCATCTGTGGGATTATGACTGAAC1819920.12883855418834536No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC1818280.12872245280538958No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCC1791190.1268046561808334No Hit
TCGATGCCGACCGGCGATGCGGCGGCG1782470.12618733663243437No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC1767320.12511481471061725No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTTC1730930.12253863829247034No Hit
TCAGTGGCTGGTTGAGAT1723930.12204308360912249No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT1718670.12167070966134967No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC1687270.11944779293890362No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCC1651810.1169374545001158No Hit
TCGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCC1645650.11650136637876968No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1628380.11527876218142435No Hit
TCTAAACCATTCGTAGACGACCTGCTT1606740.11374679027461758No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCG1589990.11256099871089235No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT1577250.11165908918719927No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1568870.1110658394377057No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT1564950.11078832881503092No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTT1553640.10998765403379318No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCGC1552600.10991402876655292No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1550560.10976960997312012No Hit
TCTAAACCATTCGTAGACGACCTGCTTC1539470.10898450976764476No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCC1486150.10520979895105799No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGG1458460.10324952621078629No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG1458040.1032197929297854No Hit
TCGGTTGGCCTCGGATAGCCGGTCCC1440770.10199718873244007No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAG1434340.10154198635902198No Hit
AATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG1425430.10091121604064637No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC1414700.10015160150460028No Hit

[OK]Adapter Content

Adapter graph