FastQCFastQC Report
Sat 1 Jul 2023
SRR2392989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2392989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33032537
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCGGAGCAAGCCCGTAGGACGTGGAATTCTCGGGTGCCAAGGAACTC485408714.694865853022431RNA PCR Primer, Index 1 (100% over 26bp)
CTAATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTTG12134563.673517417084858No Hit
TAATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTTGG11934843.6130558182679096No Hit
AGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGCCAAGGA6964962.1085150074909476RNA PCR Primer, Index 1 (100% over 22bp)
TGGGAGGAAACGCGGGGAAGTGAAACATCTCAGTACCCGTAGGTGGAATT5545591.6788265460809142No Hit
ATACCGGAGCAAGCCCGTAGGACGATGGAATTCTCGGGTGCCAAGGAACT4479461.3560750722840331RNA PCR Primer, Index 1 (100% over 25bp)
GGGTGACGCAGGAAGGTAGCTGTACCGGTTAGTGGTTTGGAATTCTCGGG4100371.241312467159274No Hit
GCGAATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGT3892641.1784259864750928No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC3042860.9211705416389907RNA PCR Primer, Index 7 (100% over 50bp)
ATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTGGAAT2986600.9041388495228205No Hit
GGCGAATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGG2163300.6548997432440626No Hit
AGGCGAATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGG2063090.6245629876990677No Hit
ATACCGGAGCAAGCCCGTAGGACGACTGGAATTCTCGGGTGCCAAGGAAC2060470.6237698303342549RNA PCR Primer, Index 1 (100% over 24bp)
TCCCCTGTAGCTCAATTGGCAGAGCATCCGATGGAATTCTCGGGTGCCAA1840080.5570507648262075No Hit
ATACCGGAGCAAGCCCGTAGGACGACATCTAGGCAAATCCGGATGTGGAA1600670.48457374012780186No Hit
CAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGCCAAGG1425550.4315593440491719Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGTGACGCAGGAAGGTAGCTGTACCGGTTAGTGGTTTGGAATTCTCGG1380220.4178365107106366No Hit
TCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGCCAAG1367530.4139948439322114No Hit
TTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGCCAA1214480.3676617390907638No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC1213280.3672984609084068No Hit
AGCCCGTCGTTGCCTAGATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC1211000.36660823236192847RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCTGTGGCGCAGCTTGGTAGCGCACTTGACTTGGAATTCTCGGGTGC1105200.3345792059507872No Hit
GTAGCAGCGGGCTCGTGGAATCTGCTGTGTGGAATTCTCGGGTGCCAAGG939880.28453158169473936Illumina Small RNA Adapter 2 (100% over 21bp)
AATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTGGAA900730.27267963099534254No Hit
ATACCGGAGCAAGCCCGTAGGACGACATGGAATTCTCGGGTGCCAAGGAA890250.26950700153609153RNA PCR Primer, Index 1 (100% over 23bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA867000.26246848675292483RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG853450.2583664706104772RNA PCR Primer, Index 7 (100% over 45bp)
ATACCGGAGCAAGCCCGTAGGACGACATCTTGGAATTCTCGGGTGCCAAG835350.2528870246932593No Hit
ATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGCCA792360.2398725838103201No Hit
TTAGCGGAAGTGGTCTGGGACGGCCCGCCGCAGACGGTGAGAGTCCGGTA786940.23823177735334106No Hit
TACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTGGAATTC696970.21099499563112575No Hit
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC688630.2084702122637447RNA PCR Primer, Index 7 (100% over 44bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC684930.20735010453481065RNA PCR Primer, Index 1 (100% over 24bp)
ATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTGGAATT669440.20266078866421916No Hit
TCCCCGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC661090.2001329779786518RNA PCR Primer, Index 1 (100% over 33bp)
ACTCCGAATGCCAATAAGTTAGAGCGCGGCAGTGAGACGGCTGGAATTCT655240.19836199683966144No Hit
ATACCGGAGCAAGCCCGTAGGACGACATCTAGGCATGGAATTCTCGGGTG644280.1950440561074676No Hit
AAGCCCGTCGTTGCCTAGATTTGTTGGAATTCTCGGGTGCCAAGGAACTC640330.19384826542387584RNA PCR Primer, Index 1 (100% over 26bp)
ATACCGGAGCAAGCCCGTAGGACTGGAATTCTCGGGTGCCAAGGAACTCC625360.18931637009897243RNA PCR Primer, Index 1 (100% over 27bp)
TAGCGGAAGTGGTCTGGGACGGCCCGCCGCAGACGGTGAGAGTCCGGTAC622820.18854743127965012No Hit
TGGGGTGACGCAGGAAGGTAGCTGTACCGGTTAGTGGTTTGGAATTCTCG612630.18546259404780202No Hit
CATGATGACCGTTGAAGTGGAGGCCCTGGAATTCTCGGGTGCCAAGGAAC609940.18464824545568512RNA PCR Primer, Index 1 (100% over 24bp)
TGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTA605420.18327989763547378RNA PCR Primer, Index 7 (100% over 47bp)
GAATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGC572200.1732231466205578No Hit
ACTGTCTCAACCATAGACTCGGCGAAATTGCACTACGAGTATGGAATTCT567800.17189112661858214No Hit
TTACGGCGGCCACAGCGAGAGGGAAACGCCCGGTCCCATCTGGAATTCTC561180.1698870419792461No Hit
CGTCATGAAAGTCGGTAACACCCGAAGCCAGTGGCCTAACCTTGGAATTC546130.1653309281088522No Hit
AGTACCTGAAACCATGTGCCTACAATCCGTCAAAGCCTCTGGAATTCTCG519630.15730853491513533No Hit
TACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTGGAATT510040.15440533677446572No Hit
CGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTGGAATTCTCG501710.15188358072527097No Hit
CGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA467840.14163005402824494RNA PCR Primer, Index 7 (100% over 37bp)
ATACCGGAGCAAGCCCGTAGGACGACATCTGGAATTCTCGGGTGCCAAGG467650.14157253498270508Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG463850.14042215407190795RNA PCR Primer, Index 7 (100% over 36bp)
AATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTTGGA459990.13925360925199295No Hit
CTCTATCCGCCGCGCGCGTCAGAAACTTGAGGAAACCTGTCCCTGGAATT454200.13750079202212048No Hit
GGTGGCTGTAGTTCAGTTGGTAGAGCACCAGGTTGTGATCCTGGCTTGGA441790.13374389015291197No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA440830.13345326760702636RNA PCR Primer, Index 7 (100% over 37bp)
CCCGTCGTTGCCTAGATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG438640.13279028492422487RNA PCR Primer, Index 1 (100% over 29bp)
CTATCCGCCGCGCGCGTCAGAAACTTGAGGAAACTGGAATTCTCGGGTGC437230.1323634330599554No Hit
CTATCCGCCGCGCGCGTCAGAAACTTGAGGAAACCTGTCCCTGGAATTCT430580.1303502664660604No Hit
GGTGGCTGTAGTTCAGTTGGTAGAGCACCAGGTGGAATTCTCGGGTGCCA429270.12995368778365404No Hit
GTTCGAGCGTCATTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC428350.12967517451051366RNA PCR Primer, Index 2 (100% over 34bp)
TTCCCCGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA418950.12682949541538394RNA PCR Primer, Index 1 (100% over 32bp)
ATGATGACCGTTGAAGTGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACT416880.12620284055081812RNA PCR Primer, Index 1 (100% over 25bp)
GGTGGCTGTAGTTCAGTTGGTAGAGCACCAGGTTGTGATCCTGGCTGGAA400990.12139243195277431No Hit
CGAATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTG395040.11959117763192093No Hit
TAATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTGGA388120.1174962734469956No Hit
CCGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG386260.11693319226434229RNA PCR Primer, Index 7 (100% over 36bp)
TTCGAGCGTCATTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA381250.11541650585300185RNA PCR Primer, Index 7 (100% over 35bp)
AATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGCC376010.11383019112337633No Hit
CCCGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA371960.11260412725792147RNA PCR Primer, Index 7 (100% over 35bp)
TAAGCCCGTCGTTGCCTAGATTTGTTGGAATTCTCGGGTGCCAAGGAACT366490.11094818421001086RNA PCR Primer, Index 1 (100% over 25bp)
GTTCGAGCGTCATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG357780.10831139006973638RNA PCR Primer, Index 7 (100% over 36bp)
TAGGACGACATCTAGGCAAATCCGGATGTGGAATTCTCGGGTGCCAAGGA334390.1012304928319614RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)