FastQCFastQC Report
Sat 1 Jul 2023
SRR2392990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2392990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24739218
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCGGAGCAAGCCCGTAGGACGTGGAATTCTCGGGTGCCAAGGAACTC14330415.79258810848427RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC13389765.412361862044306RNA PCR Primer, Index 3 (100% over 50bp)
CTAATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTTG12601585.09376650466478No Hit
TAATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTTGG6521532.636110001536831No Hit
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG4109981.6613217119474029RNA PCR Primer, Index 3 (100% over 45bp)
AGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGCCAAGGA3444031.3921337368060704RNA PCR Primer, Index 1 (100% over 22bp)
TGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTA2934601.186213727531727RNA PCR Primer, Index 3 (100% over 47bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC2826961.1427038639620701RNA PCR Primer, Index 3 (100% over 44bp)
ATACCGGAGCAAGCCCGTAGGACGATGGAATTCTCGGGTGCCAAGGAACT2553761.0322719174066053RNA PCR Primer, Index 1 (100% over 25bp)
TGGGAGGAAACGCGGGGAAGTGAAACATCTCAGTACCCGTAGGTGGAATT2454880.9923029903370431No Hit
GCGAATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGT1930950.780521841878753No Hit
GGGTGACGCAGGAAGGTAGCTGTACCGGTTAGTGGTTTGGAATTCTCGGG1926610.7787675422885234No Hit
AGCCCGTCGTTGCCTAGATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC1498050.605536521000785RNA PCR Primer, Index 1 (100% over 27bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA1485520.6004716883128642RNA PCR Primer, Index 1 (100% over 28bp)
AGGCGAATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGG1362350.5506843425689527No Hit
ATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTGGAAT1265230.511426836531373No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG1141100.4612514429518346RNA PCR Primer, Index 3 (100% over 37bp)
GGCGAATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGG1139200.4604834316104899No Hit
ATACCGGAGCAAGCCCGTAGGACGACTGGAATTCTCGGGTGCCAAGGAAC1126430.4553215869636623RNA PCR Primer, Index 1 (100% over 24bp)
TCCCCTGTAGCTCAATTGGCAGAGCATCCGATGGAATTCTCGGGTGCCAA1035560.41859043402261137No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA925200.3739811015853452RNA PCR Primer, Index 3 (100% over 36bp)
ATACCGGAGCAAGCCCGTAGGACGACATCTAGGCAAATCCGGATGTGGAA900410.3639605746632735No Hit
CGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTAT694970.2809183378391346RNA PCR Primer, Index 3 (100% over 48bp)
TCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGCCAAG688740.27840006907251474No Hit
CAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGCCAAGG681870.27562310174881033Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGTGACGCAGGAAGGTAGCTGTACCGGTTAGTGGTTTGGAATTCTCGG666260.2693132822549201No Hit
TTAGCGGAAGTGGTCTGGGACGGCCCGCCGCAGACGGTGAGAGTCCGGTA656880.26552173152764974No Hit
CGGGCTGTGGCGCAGCTTGGTAGCGCACTTGACTTGGAATTCTCGGGTGC656650.2654287617336975No Hit
AAGCCCGTCGTTGCCTAGATTTGTTGGAATTCTCGGGTGCCAAGGAACTC645780.26103492842821463RNA PCR Primer, Index 1 (100% over 26bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC620920.2509861063514619RNA PCR Primer, Index 3 (100% over 50bp)
TTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGCCAA618980.25020192635029936No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG572210.23129672085835537RNA PCR Primer, Index 3 (100% over 38bp)
TCCCCGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC532910.21541101258738252RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGAGCAAGCCCGTAGGACGACATCTTGGAATTCTCGGGTGCCAAG496580.20072582730788013No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC490160.1981307574071258No Hit
ATACCGGAGCAAGCCCGTAGGACGACATGGAATTCTCGGGTGCCAAGGAA473510.19140055275797319RNA PCR Primer, Index 1 (100% over 23bp)
ACTGTCTCAACCATAGACTCGGCGAAATTGCACTACGAGTATGGAATTCT472740.19108930605648083No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT468130.18922586801248123RNA PCR Primer, Index 3 (100% over 35bp)
CGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG455980.18431463759282932RNA PCR Primer, Index 3 (100% over 37bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG447300.1808060384123702RNA PCR Primer, Index 3 (100% over 49bp)
CCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTA446440.18045841222628783RNA PCR Primer, Index 3 (100% over 47bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT442800.17898706418286947RNA PCR Primer, Index 3 (100% over 41bp)
ATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGCCA442520.178873883564145No Hit
AATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTGGAA438210.17713171046877876No Hit
ACCACGTCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG427220.1726893711838426RNA PCR Primer, Index 3 (100% over 38bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT414880.16770133963005623RNA PCR Primer, Index 3 (100% over 46bp)
AATTCCCCGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT383450.1549968151782324RNA PCR Primer, Index 1 (100% over 30bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT382580.15464514682719557RNA PCR Primer, Index 3 (100% over 43bp)
CCGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA369520.14936607939668908RNA PCR Primer, Index 3 (100% over 36bp)
TTACGGCGGCCACAGCGAGAGGGAAACGCCCGGTCCCATCTGGAATTCTC369130.1492084349634657No Hit
TTCCCCGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA355420.1436666268109202RNA PCR Primer, Index 1 (100% over 32bp)
CCCGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT355140.14355344619219573RNA PCR Primer, Index 3 (100% over 35bp)
AATACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTTGGA351740.14217911010768408No Hit
CCCGTCGTTGCCTAGATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG350890.14183552608655617RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC342900.1386058362879538RNA PCR Primer, Index 3 (100% over 39bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG332170.13426859329183324RNA PCR Primer, Index 3 (100% over 49bp)
GTAGCAGCGGGCTCGTGGAATCTGCTGTGTGGAATTCTCGGGTGCCAAGG327720.13246982988710476Illumina Small RNA Adapter 2 (100% over 21bp)
GAATTCAGTGATCCTATGCTGCCAAGAAAAGCCTTGGAATTCTCGGGTGC322300.130278976481795No Hit
GTTCGAGCGTCATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA314630.12717863596173493RNA PCR Primer, Index 3 (100% over 36bp)
TGGGGTGACGCAGGAAGGTAGCTGTACCGGTTAGTGGTTTGGAATTCTCG314360.1270694975079649No Hit
ATACCGGAGCAAGCCCGTAGGACGACATCTAGGCATGGAATTCTCGGGTG313760.12682696761069812No Hit
TAAGCCCGTCGTTGCCTAGATTTGTTGGAATTCTCGGGTGCCAAGGAACT306850.12403383162717593RNA PCR Primer, Index 1 (100% over 25bp)
TGTTCGAGCGTCATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT304380.12303541688342776RNA PCR Primer, Index 3 (100% over 35bp)
GCCCGTCGTTGCCTAGATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA303080.12250993543934978RNA PCR Primer, Index 1 (100% over 28bp)
TTCGAGCGTCATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG299230.12095370193188806RNA PCR Primer, Index 3 (100% over 37bp)
ATTCCCCGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC293270.11854457161903824RNA PCR Primer, Index 1 (100% over 31bp)
CGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTGGAATTCTCG290900.11758657852483453No Hit
TCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT290870.1175744520299712RNA PCR Primer, Index 3 (100% over 41bp)
TACCGGATAGGACCACACACTTCATGGTGAGTGGTGCAAAGCTTGGAATT288420.11658412161613192No Hit
AGTGATCCTATGCTGCCAAGAAAAGCCTCTGGAATTCTCGGGTGCCAAGG287500.1162122424403229Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGTGTCGGAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT285130.11525424934611919RNA PCR Primer, Index 3 (100% over 34bp)
ATACCGGAGCAAGCCCGTAGGACTGGAATTCTCGGGTGCCAAGGAACTCC284130.11485003285067459RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGAGCAAGCCCGTAGGACGACATCTGGAATTCTCGGGTGCCAAGG281990.11398500955042315Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCGGAAGTGGTCTGGGACGGCCCGCCGCAGACGGTGAGAGTCCGGTAC266580.10775603335562182No Hit
AGTACCTGAAACCATGTGCCTACAATCCGTCAAAGCCTCTGGAATTCTCG259610.10493864438237295No Hit
GTAAGCCCGTCGTTGCCTAGATTTGTTGGAATTCTCGGGTGCCAAGGAAC251460.10164427994449945RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)