FastQCFastQC Report
Sat 1 Jul 2023
SRR2392995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2392995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12705538
Total Bases635.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGGGGAGAGCGGTATCGAGGTTCTCGAGGACGAATTCGTCGAGAAGT2588522.037316326156358No Hit
CCATGGCCAGGGTGAAGCGGCGGTAAGACGCCGTGGAGGCCCGAACCCAC1977811.556651910371682No Hit
CCCATGGCCAGGGTGAAGCGGCGGTAAGACGCCGTGGAGGCCCGAACCCA1465171.1531743087148298No Hit
TACGGCGGCCACAGCGAGAGGGAAACGCCCGGTCCCATCCCGAACCCGGA1204050.9476576277210772No Hit
CTGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGTTGTACTTGCCGGTTCT1170380.9211573724780486No Hit
CGGTACTAACCACCCAAAAGGTTCTAGATCAATCTCTTCGGAGTGCGACG1123250.8840633116047505No Hit
AGAGGGATAAGGTCACCCACAGACTATGGGTTCAATAGGCCAGACCTGCA954830.751506941303863No Hit
CCGCATGCATGTGATACCTGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAG930150.7320823407871433No Hit
ACCGCATGCATGTGATACCTGGTAGGGGTTGTGTGTGCGGGGTTGTGGGA892530.7024732049913982No Hit
ACCCATGGCCAGGGTGAAGCGGCGGTAAGACGCCGTGGAGGCCCGAACCC873500.6874954842526149No Hit
CCGTGAGTAGTGGCGAGCGAAAGCGGAAGATGGCTAAACCGCATGCATGT827850.6515662697636259No Hit
AGAGGGAAACAGCCCAGATCGCCGGCTAAGGCCCCTAAGCGTGTACTAAG752940.5926077274335019No Hit
CTGCGTGGGACCTTCGGTGGTAGTAGTCAAGCGATGGGGTGACGCAGGAA710490.5591970997213971No Hit
TACCCCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAAGGAATTGAC683760.538159029550736No Hit
CTCCCCGAAATGCATTTAGGTGCAGCGTCGCGTGTTTCTTGTTGGAGGTA573920.4517085384341851No Hit
TACCCATGGCCAGGGTGAAGCGGCGGTAAGACGCCGTGGAGGCCCGAACC555370.43710860571193444No Hit
GAAGGGGTGTCGCTCAACGGATAAAAGGTACCCCGGGGATAACAGGCTGA501560.3947569949418907No Hit
CCCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAAGGAATTGACGGG494480.38918462169803436No Hit
CCCTTCGGGGCAGTGGCATGGATGGGTAGGGGAGCGTCCTGCACCCAGCG490220.38583175305130724No Hit
ACCCCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAAGGAATTGACG482860.3800390034644735No Hit
AACCCTTGTCCTATGTTGCCAGCGGGTAATGCCGGGGACTCGTAGGAGAC482700.3799130741256293No Hit
GAAGGTTAAGAGGACCCGTTAACCCTTGGGTGAAGCGGAGAATTTAAGCC477330.37568657069067046No Hit
CAAGGGAGCTTGACTGCGAGACCTACAAGTCAAGCAGGGACGAAAGTCGG476460.3750018299107051No Hit
TCCGTGAGTAGTGGCGAGCGAAAGCGGAAGATGGCTAAACCGCATGCATG471240.3708933852309127No Hit
ACCAGGCCGGCGGACAGTAAAAAAGTGTCGTGATTAGCGGAAGTGGTCTG469150.3692484332422602No Hit
CGCATGCATGTGATACCTGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGT461210.3629991898021162No Hit
CCCCCGTAACTTCGGGAGAAGGGGGACCTCGCTTGGTGACCGGACTTGCT460700.3625977900345503No Hit
ACTGCAGGGGAGACTGGAATTCCTGGTGTAGCGGTGGAATGCGCAGATAT452870.3564351230148617No Hit
ACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCTCTATCCGCCG429810.3382855570539398No Hit
TACCAGGCCGGCGGACAGTAAAAAAGTGTCGTGATTAGCGGAAGTGGTCT426180.33542853517891175No Hit
CAAGGGTTCCTGGGCCAGGCTAGTCCTCCCAGGGTAAGTCGGGACCTAAG421590.33181593727081843No Hit
CGAGGTTAACCCGTGTGGGGTAGCCGTAGCGAAAGCGAGTCTGAATAGGG401180.3157520759845038No Hit
CCTCGGGGAGCTGCCAACCGAGCTTTGATCCGAGGATCTCCGAATGGGGA389780.3067796105918537No Hit
CCCCGAAATGCATTTAGGTGCAGCGTCGCGTGTTTCTTGTTGGAGGTAGA384250.3024271778180507No Hit
GTTACGGCGGCCACAGCGAGAGGGAAACGCCCGGTCCCATCCCGAACCCG383270.3016558606176299No Hit
AGCGGAAGTGGTCTGGGACGGCCCGCCGCAGACGGTGAGAGTCCGGTACG370930.2919435603592701No Hit
CACGGCGGAGGTCGCGGGTTCGAGTCCCGTCAGGGTCGCCAAAAAAAAAA356790.28081455503891295No Hit
TCCCCGAAATGCATTTAGGTGCAGCGTCGCGTGTTTCTTGTTGGAGGTAG354860.27929553238910465No Hit
GTGCAGTCGCGAAGACAACCAGGAGGTTGGCTTAGAAGCAGCCACCCTTG354410.2789413561236053No Hit
AGGGTGAAGCGGCGGTAAGACGCCGTGGAGGCCCGAACCCACTTAGGTTG347880.2738018649820259No Hit
ACCCCCGTAACTTCGGGAGAAGGGGGACCTCGCTTGGTGACCGGACTTGC330860.260406131562473No Hit
CAGGGTGAAGCGGCGGTAAGACGCCGTGGAGGCCCGAACCCACTTAGGTT329700.2594931438558524No Hit
AAAGGTTCCCTCAACCTGGACGGCAATCAGGTGTTGAGTGCAAGTGCACA323970.2549832994084942No Hit
TGAGTGCAAGTGCACAAGGGAGCTTGACTGCGAGACCTACAAGTCAAGCA318040.2503160432875806No Hit
GAAGGTGGGGATGACGTCAAGTCATCATGCCCCTTATGTCCAGGGCTTCA298340.2348109934423871No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATGGTAAGGAAAAGGTCAA297280.23397671157254418No Hit
CCCCGTAACTTCGGGAGAAGGGGGACCTCGCTTGGTGACCGGACTTGCTC291330.22929371428427509No Hit
CACCGACAACGGATCCGTTTTCGTAGTGGGTGGCGGCAAGGTGCAGCGCG279830.22024254305484742No Hit
GAAGGGGGACCTCGCTTGGTGACCGGACTTGCTCCGTGAGCTGAACGAGG275820.21708643900006439No Hit
CATGGCCAGGGTGAAGCGGCGGTAAGACGCCGTGGAGGCCCGAACCCACT274960.21640956880377674No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT269180.21186037143802963No Hit
CAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGA268800.2115612892582746No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG265040.20860194979543567No Hit
TGGCCAGTACAGAGGGCTGCGAAGCCGTAAGGTGGAGCGAATCCCTTAAA258940.20380089375200014No Hit
CACTTAGGTTGAAGACTGAGGGGATGAGCTGTGGGTAGGGGTGAAAGGCC258100.203139764723068No Hit
TACTGCAGGGGAGACTGGAATTCCTGGTGTAGCGGTGGAATGCGCAGATA240780.18950791379318216No Hit
GAAGGACCGGCCAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTCCG235350.18523418685615675No Hit
CCCCGTCGTCTAGCGGCCTAGGACGCCGCCCTCTCACGGCGGTAGCGTGG233530.18380174062680385No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCTCATAACCCAGAGGTCG232690.18314061159787173No Hit
GCCCGCCGCAGACGGTGAGAGTCCGGTACGCGAAAATCCGACACCTGTCT230860.18170029478484107No Hit
TGGGGTAGCCGTAGCGAAAGCGAGTCTGAATAGGGCGCCCATAGTGGCGT225570.17753675601930433No Hit
GAGAGGGAAACGCCCGGTCCCATCCCGAACCCGGAAGCTAAGCCTCTCAG225310.1773321208436825No Hit
CTCGCTTGGTGACCGGACTTGCTCCGTGAGCTGAACGAGGTCGCAGAGAC224010.17630894496557328No Hit
CCCCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAAGGAATTGACGG217140.17090185397894997No Hit
CGAGGTCGCAGAGACCAGTGAGAAGCGACTGTTTACTAAAAACACAGGTC212040.16688785630329073No Hit
CCCGAACCCACTTAGGTTGAAGACTGAGGGGATGAGCTGTGGGTAGGGGT210540.16570726875162625No Hit
TCCTGCACCCAGCGAAGCTGCGAAGTAATTCAGCAGTGGAGGGTGCGGGA208830.16436139894272875No Hit
CTGCACCCAGCGAAGCTGCGAAGTAATTCAGCAGTGGAGGGTGCGGGAGT206570.16258264703155428No Hit
CCGGTGCAGGCAAGAGACCACCGTAAGCGTCATTGCAACCAATTAAGCGC203580.16022934251190307No Hit
CGCGTGTTTCTTGTTGGAGGTAGAGCTACTGGATGGCCGATGGGCCCTAC200230.1575926969798524No Hit
GAAGGTAGCTGTACCGGTTAGTGGTTATACCGGAGCAAGCCCGTAGGACG197690.15559356872570054No Hit
TCCCTTCGGGGCAGTGGCATGGATGGGTAGGGGAGCGTCCTGCACCCAGC191100.15040685408205462No Hit
GGCCAGTACAGAGGGCTGCGAAGCCGTAAGGTGGAGCGAATCCCTTAAAG190240.149729983885767No Hit
TGTGCAGTCGCGAAGACAACCAGGAGGTTGGCTTAGAAGCAGCCACCCTT190050.14958044279588947No Hit
CTACGTGCCAGCAGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAA185080.1456687627080412No Hit
GGGGATGAGCTGTGGGTAGGGGTGAAAGGCCAATCAAACTCCGTGATAGC180530.1420876471346589No Hit
TCGGTACTAACCACCCAAAAGGTTCTAGATCAATCTCTTCGGAGTGCGAC179430.14122188293010499No Hit
AAGGGGTGTCGCTCAACGGATAAAAGGTACCCCGGGGATAACAGGCTGAT179400.1411982711790717No Hit
GTACCCCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAAGGAATTGA167100.13151745325542297No Hit
GCGGGGAAGTGAAACATCTCAGTACCCGTAGGAAGAGAAAACAAAAAAAA166860.13132855924715664No Hit
AAAGTACCCCGGGAGGGGAGTGAAATAGTACCTGAAACCATGTGCCTACA164910.1297937954299928No Hit
CCTCCAGGGAGGGCCGGCAGTGGCCCAGGTGCGACGAATGGCGACGGAAG162840.12816458460869584No Hit
GCCAGTACAGAGGGCTGCGAAGCCGTAAGGTGGAGCGAATCCCTTAAAGC161990.12749558499608596No Hit
TCGCGTGTTTCTTGTTGGAGGTAGAGCTACTGGATGGCCGATGGGCCCTA161900.1274247497429861No Hit
AACGGAGGCGCCCAAAGGTTCCCTCAACCTGGACGGCAATCAGGTGTTGA160560.12637009153016582No Hit
TCCTGGTGTAGCGGTGGAATGCGCAGATATCAGGAGGAACACCGGTGGCG159680.12567748016652266No Hit
AAAGGATGTGCAGTCGCGAAGACAACCAGGAGGTTGGCTTAGAAGCAGCC157320.12382002241857054No Hit
CCGGGGAGAGCGGTATCGAGGTTCTCGAGGACGAATTCGTCGAGAAGTTC157160.12369409307972634No Hit
CGAGGATCTCCGAATGGGGAAACCCAGCACGAGTGATGTCGTGTTACCCA156800.12341075206732685No Hit
CAAGTGATTGTGCGCCGACAATGTAGCGGGGCTCAAGTACACCGCCGAAG153670.12094725937618699No Hit
AAAGGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCATTTAG149450.11762587306417091No Hit
ACGGCGGCCACAGCGAGAGGGAAACGCCCGGTCCCATCCCGAACCCGGAA147240.11588647407138526No Hit
AGGTTCTAGATCAATCTCTTCGGAGTGCGACGTGAACCCGCTGCGTGGGA147240.11588647407138526No Hit
AAAGGTTCTAGATCAATCTCTTCGGAGTGCGACGTGAACCCGCTGCGTGG146900.11561887422634132No Hit
GGTTCTAGATCAATCTCTTCGGAGTGCGACGTGAACCCGCTGCGTGGGAC143780.11316325211887918No Hit
CAAGGGTTGGGCTGTTCGCCCATTAAAGCGGCACGCGAGCTGGGTTTAGA141580.11143172370977128No Hit
GTGGGTGATCTGCCCTGCACTCTGGGATAAGCCTGGGAAACTGGGTCTAA139410.10972380705169668No Hit
CTACCCATGGCCAGGGTGAAGCGGCGGTAAGACGCCGTGGAGGCCCGAAC138850.10928305436574194No Hit
CCAGGCCGGCGGACAGTAAAAAAGTGTCGTGATTAGCGGAAGTGGTCTGG138080.10867701942255416No Hit
CTGCAAGCGTAGTAATACGTTCAGGGAACTGGCACTAATCGACCGAAAAC136820.107685325879156No Hit
TCCCGCAACGAGCGCAACCCTTGTCCTATGTTGCCAGCGGGTAATGCCGG136420.10737050253204548No Hit
TAAGGCGTACGAGATAACTATGGTTAAGGAACTCGGCAAAATACCCCCGT135850.10692187926241298No Hit
CCTCGTGGGGTGATGGCGTGCCTTTTGAAGAATGAGCCTGCGAGTCAGGG133690.10522183318801613No Hit
CCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTGG132850.10456070415908401No Hit
AGGGGTGTCGCTCAACGGATAAAAGGTACCCCGGGGATAACAGGCTGATC131480.10348243419523045No Hit
ATGGGTAGGGGAGCGTCCTGCACCCAGCGAAGCTGCGAAGTAATTCAGCA130460.10267963466009862No Hit
CCGTGGGTCAGCAGGGCCCCGAGCTTTGAAGAATTGCCCTGCGCAGTCGT130110.10240416423137691No Hit
GAAGGCGGGTCTCTGGGCAGTAACTGACGCTGAGGAGCGAAAGCGTGGGT128850.10141247068797875No Hit
CCGCGGCATTCATGCAATACATTCCCTTCGGGGCAGTGGCATGGATGGGT128130.10084578866317978No Hit
CCCGGGAGGGGAGTGAAATAGTACCTGAAACCATGTGCCTACAATCCGTC127080.10001937737701466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)